Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
August-2017 Volume 14 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2017 Volume 14 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy

  • Authors:
    • Shuwen Li
    • Fang Wang
    • Zhenxing Zhai
    • Shengjun Fu
    • Jianzhong Lu
    • Hongjuan Zhang
    • Hongyu Guo
    • Xuemei Hu
    • Renju Li
    • Zhiping Wang
    • Ronald Rodriguez
  • View Affiliations / Copyright

    Affiliations: Urologic Clinical Center of Gansu Province, Key Laboratory of Gansu Province, Institute of Urology, The Second Hospital of Lanzhou University, Lanzhou, Gansu 730030, P.R. China, Medical Experiment Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Department of Urology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2081-2088
    |
    Published online on: June 19, 2017
       https://doi.org/10.3892/ol.2017.6416
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Gene therapy with adenoviral early region gene (E1A) may enhance the susceptibility of neoplastic cells to chemotherapy-induced cell death. Our previous study developed a urothelium‑specific oncolytic serotype 5 adenovirus (Ad5) with the uroplakin II (UPII) promoter controlling E1A expression. The present study investigated whether this urothelium-specific recombinant adenovirus (Ad5‑UPII‑E1A) enhanced mitomycin (MMC) and hydroxycamptothecin (HCPT) sensitization and drug‑induced apoptosis in bladder cancer cells. The results of the MTT assay revealed that combination therapy, using Ad5‑UPII‑E1A and MMC or HCPT, synergistically inhibited the viability of bladder cancer cells in a dose‑ and time‑dependent manner when compared with either agent alone. When cells were treated with Ad5‑UPII‑E1A alone they arrested in the G1 phase, but cell cycle analysis by flow cytometry revealed S phase arrest when treated with combined therapy. Treatment with MMC or HCPT enhanced Ad5‑UPII‑E1A‑induced apoptosis in 5,637 cells, observed by transmission electron microscopy. Western blot analysis revealed that MMC and HCPT enhanced the E1A expression of the Ad5‑UPII‑E1A vectorin a dose‑dependent manner. The present study demonstrated that Ad5‑UPII‑E1A combined with MMC or HCPT resulted in synergistic cytotoxicity in a process which involved the promotion of apoptosis in bladder cancer cell lines. MMC and HCPT also promoted the oncolytic effect of Ad5‑UPII‑E1A. Thus, treatment using Ad5‑UPII‑E1A combined with MMC or HCPT may be an attractive strategy for the sensitization of bladder cancer to chemotherapy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Cheung G, Sahai A, Billia M, Dasgupta P and Khan MS: Recent advances in the diagnosis and treatment of bladder cancer. BMC Med. 11:132013. View Article : Google Scholar : PubMed/NCBI

2 

Sylvester RJ, van der Meijden AP, Oosterlinck W, Witjes JA, Bouffioux C, Denis L, Newling DW and Kurth K: Predicting recurrence and progression in individual patients with stage Ta T1 bladder cancer using EORTC risk tables: A combined analysis of 2596 patients from seven EORTC trials. Eur Urol. 49:466–477. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Voutsinas GE and Stravopodis DJ: Molecular targeting and gene delivery in bladder cancer therapy. J Buon. 14 Suppl 1:69–78. 2009.

4 

Babjuk M, Oosterlinck W, Sylvester R, Kaasinen E, Böhle A and Palou-Redorta J; European Association of Urology (EAU), : EAU guidelines on non-muscle-invasive urothelial carcinoma of the bladder. Eur Urol. 54:303–314. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Crooke ST and Bradner WT: Mitomycin C: A review. Cancer Treat Rev. 3:121–139. 1976. View Article : Google Scholar : PubMed/NCBI

6 

Verweij J and Pinedo HM: Mitomycin C: Mechanism of action, usefulness and limitations. Anticancer Drugs. 1:5–13. 1990. View Article : Google Scholar : PubMed/NCBI

7 

Wu Y, Zeng FQ, Wang YB and Wang L: Hydroxycamptothecin promotes the apoptosis of prostate cancer cell line PC-3. Zhonghua Nan KeXue. 13:890–894. 2007.(In Chinese).

8 

Fan J, Tang X and Zhang X: 10-Hydroxycamptothecin induces apoptosis in human T24 urinary bladder cancer cells. Zhonghua Yi XueZaZhi. 78:301–304. 1998.(In Chinese).

9 

Liu LF, Desai SD, Li TK, Mao Y, Sun M and Sim SP: Mechanism of action of camptothecin. Ann N Y Acad Sci. 922:1–10. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Dean M, Fojo T and Bates S: Tumour stem cells and drug resistance. Nat Rev Cancer. 5:275–284. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Bochner BH: Gene therapy in bladder cancer. Curr Opin Urol. 18:519–23. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Wada Y, Gotoh A, Shirakawa T, Hamada K and Kamidono S: Gene therapy for bladder cancer using adenoviral vector. Mol Urol. 5:47–52. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Chester JD, Kennedy W, Hall GD, Selby PJ and Knowles MA: Adenovirus-mediated gene therapy for bladder cancer: Efficient gene delivery to normal and malignant human urothelial cells in vitro and ex vivo. Gene Ther. 10:172–179. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Wang F, Wang Z, Tian H, Qi M, Zhai Z, Li S, Li R, Zhang H, Wang W, Fu S, et al: Biodistribution and safety assessment of bladder cancer specific recombinant oncolytic adenovirus in subcutaneous xenografts tumor model in nude mice. Curr Gene Ther. 12:67–76. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Wang D, Wang Z, Tian J, He X, Chowdhury WH, Zhang X, Li S and Rodriguez R: Prostate stem cell antigen enhancer and uroplakin II promoter based bladder cancer targeted tissue-specific vector. Urol Oncol. 28:164–169. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Zhai Z, Wang Z, Fu S, Lu J, Wang F, Li R, Zhang H, Li S, Hou Z, Wang H and Rodriguez R: Antitumor effects of bladder cancer-specific adenovirus carrying E1A-andro gen receptor in bladder. cancer. 19:1065–1074. 2012.

17 

Guo W, Zhu H, Zhang L, Davis J, Teraishi F, Roth JA, Stephens C, Fueyo J, Jiang H, Conrad C and Fang B: Combination effect of oncolytic adenovirotherapy and TRAIL gene therapy in syngeneic murine breast cancer models. Cancer GeneTher. 13:82–90. 2006.

18 

Alonso MM, Gomez-Manzano C, Jiang H, Bekele NB, Piao Y, Yung WK, Alemany R and Fueyo J: Combination of the oncolytic adenovirus ICOVIR-5 with chemotherapy provides enhanced anti-glioma effect in vivo. Cancer Gene Ther. 14:756–761. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Zhou JR, Yu L, Mai Z and Blackburn GL: Combined inhibition of estrogen-dependent human breast carcinoma by soy and tea bioactive components in mice. Int J Cancer. 108:8–14. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Sánchez-Prieto R, Quintanilla M, Cano A, Leonart ML, Martin P, Anaya A and Ramón y Cajal S: Carcinoma cell lines become sensitive to DNA-damaging agents by the expression of the adenovirus E1A gene. Oncogene. 13:1083–1092. 1996.PubMed/NCBI

21 

Ganjavi H, Gee M, Narendran A, Parkinson N, Krishnamoorthy M, Freedman MH and Malkin D: Adenovirus-mediated p53 gene therapy in osteosarcoma cell lines: Sensitization to cisplatin and doxorubicin. Cancer Gene Ther. 13:415–419. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Brader KR, Wolf JK, Hung MC, Yu D, Crispens MA, van Golen KL and Price JE: Adenovirus E1A expression enhances the sensitivity of an ovarian cancer cell line to multiple cytotoxic agents through an apoptotic mechanism. Clin Cancer Res. 3:2017–2024. 1997.PubMed/NCBI

23 

Ueno NT, Bartholomeusz C, Herrmann JL, Estrov Z, Shao R, Andreeff M, Price J, Paul RW, Anklesaria P, Yu D, Hung MC, et al: E1A-mediated paclitaxel sensitization in HER-2/neu-overexpressing ovarian cancer SKOV3.ip1 through apoptosis involving the caspase-3 pathway. Clin Cancer Res. 6:250–259. 2000.PubMed/NCBI

24 

Liao Y and Hung MC: Regulation of the activity of p38 mitogen-activated protein kinase by Akt in cancer and adenoviral protein E1A-mediated sensitization to apoptosis. Mol Cell Biol. 23:6836–6848. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Liao Y and Hung MC: A new role of protein phosphatase 2a in adenoviral E1A protein-mediated sensitization to anticancer drug-induced apoptosis in human breast cancer cells. Cancer Res. 64:5938–5942. 2004. View Article : Google Scholar : PubMed/NCBI

26 

de Stanchina E, McCurrach ME, Zindy F, Shieh SY, Ferbeyre G, Samuelson AV, Prives C, Roussel MF, Sherr CJ and Lowe SW: E1A signaling to p53 involves the p19(ARF) tumor suppressor. Genes Dev. 12:2434–2442. 1998. View Article : Google Scholar : PubMed/NCBI

27 

Duelli DM and Lazebnik YA: Primary cells suppress oncogene-dependent apoptosis. Nat Cell Biol. 2:859–862. 2000. View Article : Google Scholar : PubMed/NCBI

28 

McCurrach ME, Connor TM, Knudson CM, Korsmeyer SJ and Lowe SW: bax-deficiency promotes drug resistance and oncogenic transformation by attenuating p53-dependent apoptosis. Proc Natl Acad Sci USA. 94:pp. 2345–2349. 1997; View Article : Google Scholar : PubMed/NCBI

29 

Su JL, Cheng X, Yamaguchi H, Chang YW, Hou CF, Lee DF, Ko HW, Hua KT, Wang YN, Hsiao M, et al: FOXO3a dependent mechanism of E1A-Induced chemosensitization. Cancer Res. 71:6878–6887. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Olivier M and Theillet C: Mitomycin C induced apoptosis: influence of cell cycle phase. Biology of the Cell. 88:82–82a. 1996. View Article : Google Scholar

31 

Hu W, Zhang C, Fang Y and Lou C: Anticancer properties of 10-hydroxycamptothecin in a murine melanoma pulmonary metastasis model in vitro and in vivo. Toxicol In Vitro. 25:513–520. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Tanaka M and Grossman HB: Connexin 26 gene therapy of human bladder cancer: Induction of growth suppression, apoptosis, and synergy with Cisplatin. Hum Gene Ther. 12:2225–2236. 2001. View Article : Google Scholar : PubMed/NCBI

33 

Li Y, Yu DC, Chen Y, Amin P, Zhang H, Nguyen N and Henderson DR: A hepatocellular carcinoma-specific adenovirus variant, CV890, eliminates distant human liver tumors in combination with doxorubicin. Cancer Res. 61:6428–6436. 2001.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li S, Wang F, Zhai Z, Fu S, Lu J, Zhang H, Guo H, Hu X, Li R, Wang Z, Wang Z, et al: Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy. Oncol Lett 14: 2081-2088, 2017.
APA
Li, S., Wang, F., Zhai, Z., Fu, S., Lu, J., Zhang, H. ... Rodriguez, R. (2017). Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy. Oncology Letters, 14, 2081-2088. https://doi.org/10.3892/ol.2017.6416
MLA
Li, S., Wang, F., Zhai, Z., Fu, S., Lu, J., Zhang, H., Guo, H., Hu, X., Li, R., Wang, Z., Rodriguez, R."Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy". Oncology Letters 14.2 (2017): 2081-2088.
Chicago
Li, S., Wang, F., Zhai, Z., Fu, S., Lu, J., Zhang, H., Guo, H., Hu, X., Li, R., Wang, Z., Rodriguez, R."Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy". Oncology Letters 14, no. 2 (2017): 2081-2088. https://doi.org/10.3892/ol.2017.6416
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Wang F, Zhai Z, Fu S, Lu J, Zhang H, Guo H, Hu X, Li R, Wang Z, Wang Z, et al: Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy. Oncol Lett 14: 2081-2088, 2017.
APA
Li, S., Wang, F., Zhai, Z., Fu, S., Lu, J., Zhang, H. ... Rodriguez, R. (2017). Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy. Oncology Letters, 14, 2081-2088. https://doi.org/10.3892/ol.2017.6416
MLA
Li, S., Wang, F., Zhai, Z., Fu, S., Lu, J., Zhang, H., Guo, H., Hu, X., Li, R., Wang, Z., Rodriguez, R."Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy". Oncology Letters 14.2 (2017): 2081-2088.
Chicago
Li, S., Wang, F., Zhai, Z., Fu, S., Lu, J., Zhang, H., Guo, H., Hu, X., Li, R., Wang, Z., Rodriguez, R."Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy". Oncology Letters 14, no. 2 (2017): 2081-2088. https://doi.org/10.3892/ol.2017.6416
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team