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Anticancer effects of an extract from the scallop Patinopecten yessoensis on MCF‑7 human breast carcinoma cells

  • Authors:
    • Chu Lee
    • Wonjoo Chun
    • Rongjie Zhao
    • Young Dae Kim
    • Myung Mo Nam
    • Dae Hwa Jung
    • Il Je Cho
    • Kyung Hwan Jegal
    • Tae Hoon Lee
    • Young Woo Kim
    • Sang Mi Park
    • Seong A. Ju
    • Chul Won Lee
    • Sang Chan Kim
    • Won G. An
  • View Affiliations / Copyright

    Affiliations: Aquaculture Industry Division, NFRDI, Gangneung 210‑809, Republic of Korea, Institute of Marine Biotechnology, Pusan National University, Busan 609‑735, Republic of Korea, School of Mental Health, Qiqihar Medical University, Qiqihar, Heilongjiang 161042, P.R. China, HaniBio Co., Ltd., Gyeongsan 712‑260, Republic of Korea, MRC‑GHF, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea, Department of Biological Sciences, College of Biomedical Sciences and Engineering, Inje University, Gimhae 621‑749, Republic of Korea, School of Biological Sciences, University of Ulsan, Ulsan 680‑749, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2207-2217
    |
    Published online on: June 20, 2017
       https://doi.org/10.3892/ol.2017.6424
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Abstract

Patinopecten yessoensis, is a species of scallop and a marine bivalve mollusk. In traditional East Asian medicine, scallop meat is used as a drug for the treatment of diabetes, pollakisuria, and indigestion. The present study was conducted in order to examine the potential anticancer effects of scallop flesh extract (SE) on MCF‑7 human breast cancer cells. An MTT assay was used to evaluate cell viability and flow cytometry was used for the assessment of cell cycle distribution and apoptosis. The alteration in protein expression level was determined by western blot analysis, and the amounts of docosahexaenoic acid and eicosapentaenoic acid in the SE were measured by gas chromatography. SE inhibited the growth of MCF‑7 human breast cancer cells in a dose‑dependent manner by inducing G0/G1 phase arrest. The cell cycle arrest was associated with the upregulation of p53 and p21, and downregulation of G1 phase‑associated cyclin D1/cyclin‑dependent kinase (Cdk) 4 and cyclin E1/Cdk 2. In addition, SE‑mediated cell cycle arrest was associated with the promotion of apoptosis, as indicated by the expression of apoptosis‑associated proteins and changes in nuclear morphology. SE appeared to induce the mitochondrial apoptotic cascade, as indicated by a decreased expression of Bcl‑2, activation of Bcl‑2 associated X protein, release of cytochrome c, decrease in procaspase‑3, and an increase in cleaved‑poly (ADP‑ribose) polymerase (PARP). Furthermore, the expression levels of Fas‑associated via death domain and cleaved caspase‑8 were increased in a SE dose‑dependent manner. Taken together, these results suggest that the intrinsic and extrinsic pathways of apoptosis are associated with the anticancer effects of SE on MCF‑7 cells. Thus, SE may be a suitable candidate for the treatment and prevention of human breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Lee C, Chun W, Zhao R, Kim YD, Nam MM, Jung DH, Cho IJ, Jegal KH, Lee TH, Kim YW, Kim YW, et al: Anticancer effects of an extract from the scallop Patinopecten yessoensis on MCF‑7 human breast carcinoma cells. Oncol Lett 14: 2207-2217, 2017.
APA
Lee, C., Chun, W., Zhao, R., Kim, Y.D., Nam, M.M., Jung, D.H. ... An, W.G. (2017). Anticancer effects of an extract from the scallop Patinopecten yessoensis on MCF‑7 human breast carcinoma cells. Oncology Letters, 14, 2207-2217. https://doi.org/10.3892/ol.2017.6424
MLA
Lee, C., Chun, W., Zhao, R., Kim, Y. D., Nam, M. M., Jung, D. H., Cho, I. J., Jegal, K. H., Lee, T. H., Kim, Y. W., Park, S. M., Ju, S. A., Lee, C. W., Kim, S. C., An, W. G."Anticancer effects of an extract from the scallop Patinopecten yessoensis on MCF‑7 human breast carcinoma cells". Oncology Letters 14.2 (2017): 2207-2217.
Chicago
Lee, C., Chun, W., Zhao, R., Kim, Y. D., Nam, M. M., Jung, D. H., Cho, I. J., Jegal, K. H., Lee, T. H., Kim, Y. W., Park, S. M., Ju, S. A., Lee, C. W., Kim, S. C., An, W. G."Anticancer effects of an extract from the scallop Patinopecten yessoensis on MCF‑7 human breast carcinoma cells". Oncology Letters 14, no. 2 (2017): 2207-2217. https://doi.org/10.3892/ol.2017.6424
Copy and paste a formatted citation
x
Spandidos Publications style
Lee C, Chun W, Zhao R, Kim YD, Nam MM, Jung DH, Cho IJ, Jegal KH, Lee TH, Kim YW, Kim YW, et al: Anticancer effects of an extract from the scallop Patinopecten yessoensis on MCF‑7 human breast carcinoma cells. Oncol Lett 14: 2207-2217, 2017.
APA
Lee, C., Chun, W., Zhao, R., Kim, Y.D., Nam, M.M., Jung, D.H. ... An, W.G. (2017). Anticancer effects of an extract from the scallop Patinopecten yessoensis on MCF‑7 human breast carcinoma cells. Oncology Letters, 14, 2207-2217. https://doi.org/10.3892/ol.2017.6424
MLA
Lee, C., Chun, W., Zhao, R., Kim, Y. D., Nam, M. M., Jung, D. H., Cho, I. J., Jegal, K. H., Lee, T. H., Kim, Y. W., Park, S. M., Ju, S. A., Lee, C. W., Kim, S. C., An, W. G."Anticancer effects of an extract from the scallop Patinopecten yessoensis on MCF‑7 human breast carcinoma cells". Oncology Letters 14.2 (2017): 2207-2217.
Chicago
Lee, C., Chun, W., Zhao, R., Kim, Y. D., Nam, M. M., Jung, D. H., Cho, I. J., Jegal, K. H., Lee, T. H., Kim, Y. W., Park, S. M., Ju, S. A., Lee, C. W., Kim, S. C., An, W. G."Anticancer effects of an extract from the scallop Patinopecten yessoensis on MCF‑7 human breast carcinoma cells". Oncology Letters 14, no. 2 (2017): 2207-2217. https://doi.org/10.3892/ol.2017.6424
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