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Article Open Access

Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents

  • Authors:
    • Dongyan Huang
    • Jianhui Wu
    • Xin Su
    • Han Yan
    • Zuyue Sun
  • View Affiliations / Copyright

    Affiliations: School of Pharmacy, Fudan University, Shanghai 200433, P.R. China, National Evaluation Centre for The Toxicology of Fertility Regulating Drugs, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2635-2642
    |
    Published online on: June 23, 2017
       https://doi.org/10.3892/ol.2017.6469
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Abstract

Bisphenol A (BPA) is a well-known endocrine disruptor compound (EDC) that aggravates testosterone‑induced benign prostate hyperplasia by increasing the relative weight of the ventral and dorsolateral prostate in rats. This phenomenon is primarily attributed to the exogenous estrogen effect of BPA. However, the direct effect of BPA on prostate cells has not been characterized. The present study investigated the proliferative effect and possible mechanisms of action of BPA on the prostatic epithelium of rats. The ventral prostate epithelial cells were cultured in vitro and the proliferation effects of BPA on cells were studied. The cells were identified as prostatic epithelial cells, and cell viability, cell apoptosis and the expressions of androgen receptors (AR) and estrogen receptors (ER), were detected. It was observed that 0.01‑1 nM BPA promoted cell growth, with 1 nM BPA inducing the greatest increase in the rate of cell growth. However, BPA‑treated cells exhibited no marked morphological changes compared with the control group. The cell apoptosis rate in each BPA‑treated group was lower compared with the control group. The expression levels of ERα and ERβ increased, but the expression of AR decreased. The present study demonstrated that environmental exposure to BPA directly promoted the proliferation of prostate cells in rats through increasing the expression of estrogen receptors, reducing the expression of androgen receptors of the cells and decreasing apoptosis‑induced cell death.
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View References

1 

Alonso-Magdalena P, Brössner C, Reiner A, Cheng G, Sugiyama N, Warner M and Gustafsson JA: A role for epithelial-mesenchymal transition in the etiology of benign prostatic hyperplasia. Proc Natl Acad Sci USA. 106:pp. 2859–2863. 2009; View Article : Google Scholar : PubMed/NCBI

2 

Tyl RW, Myers CB, Marr MC, Thomas BF, Keimowitz AR, Brine DR, Veselica MM, Fail PA, Chang TY, Seely JC, et al: Three-generation reproductive toxicity study of dietary bisphenol A in CD Sprague-Dawley rats. Toxicol Sci. 68:121–146. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Biedermann S, Tschudin P and Grob K: Transfer of bisphenol A from thermal printer paper to the skin. Anal Bioanal Chem. 398:571–576. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Vandenberg LN, Chahoud I, Heindel JJ, Padmanabhan V, Paumgartten FJ and Schoenfelder G: Urinary, circulating, and tissue biomonitoring studies indicate widespread exposure to bisphenol A. Environ Health Perspect. 118:1055–1070. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Vandenberg LN, Colborn T, Hayes TB, Heindel JJ, Jacobs DR Jr, Lee DH, Shioda T, Soto AM, vom Saal FS, Welshons WV, et al: Hormones and endocrine-disrupting chemicals: Low-dose effects and nonmonotonic dose responses. Endocr Rev. 33:378–455. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Ramos JG, Varayoud J, Sonnenschein C, Soto AM, Muñoz-de-Toro M and Luque EH: Prenatal exposure to low doses of bisphenol A alters the periductal stroma and glandular cell function in the rat ventral prostate. Biol Reprod. 65:1271–1277. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Derouiche S, Warnier M, Mariot P, Gosset P, Mauroy B, Bonnal JL, Slomianny C, Delcourt P, Prevarskaya N and Roudbaraki M: Bisphenol A stimulates human prostate cancer cell migration via remodelling of calcium signalling. Springerplus. 2:542013. View Article : Google Scholar : PubMed/NCBI

8 

Takahashi O and Oishi S: Testicular toxicity of dietary 2,2-bis(4-hydroxyphenyl)propane (bisphenol A) in F344 rats. Arch Toxicol. 75:42–51. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Herath CB, Jin W, Watanabe G, Arai K, Suzuki AK and Taya K: Adverse effects of environmental toxicants, octylphenol and bisphenol A, on male reproductive functions in pubertal rats. Endocrine. 25:163–172. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Ogura Y, Ishii K, Kanda H, Kanai M, Arima K, Wang YZ and Sugimura Y: Bisphenol A induces permanent squamous change in mouse prostatic epithelium. Differentiation. 75:745–756. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Ho SM, Tang WY, de Frausto J Belmonte and Prins GS: Developmental exposure to estradiol and bisphenol A increases susceptibility to prostate carcinogenesis and epigenetically regulates phosphodiesterase type 4 variant 4. Cancer Res. 66:5624–5632. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Wu J, Huang D, Su X, Yan H and Sun ZY: Oral administration of low-dose bisphenol A promotes proliferation of ventral prostate and upregulates prostaglandin D2 synthase expression in adult rats. Toxicol Ind Health. pii:Jun 18–2015.(Epub ahead of print).

13 

Wang JF, Zhou YLIUJH, et al: Guide for the Care and Use of Laboratory Animals. Shanghai Scientific & Technical Publishers (SSTP); Shanghai, China: 2012, View Article : Google Scholar : PubMed/NCBI

14 

Mckeehan WL, Adams PS and Rosser MP: Direct mitogenic effects of insulin, epidermal growth factor, glucocorticoid, cholera toxin, unknown pituitary factors and possibly prolactin, but not androgen, on normal rat prostate epithelial cells in serum-free, primary cell culture. Cancer Res. 44:1998–2010. 1984.PubMed/NCBI

15 

Wu JH, Jiang XR, Liu GM, Liu XY, He GL and Sun ZY: Oral exposure to low-dose bisphenol A aggravates testosterone-induced benign hyperplasia prostate in rats. Toxicol Ind Health. 27:810–819. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Gualtieri AF, Iwachow MA, Venara M, Rey RA and Schteingart HF: Bisphenol A effect onglutathione synthesis and recycling in testicular Sertoli cells. J Endocrinol. 5:e102–e109. 2011.

17 

Wetherill YB, Hess-Wilson JK, Comstock CE, Shah SA, Buncher CR, Sallans L, Limbach PA, Schwemberger S, Babcock GF and Knudsen KE: Bisphenol A facilitates bypass of androgen ablation therapy in prostate cancer. Mol Cancer Ther. 5:3181–3190. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Hess-Wilson JK, Webb SL, Daly HK, Leung YK, Boldison J, Comstock CE, Sartor MA, Ho SM and Knudsen KE: Unique bisphenol A tanscriptom in prostate cancer: Novel effects on ERbeta expression that correspond to androgen receptor mutation status. Environ Health Perspect. 115:1646–1653. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Mokra K, Kocia M and Michałowicz J: Bisphenol A and its analogs exhibit different apoptotic potential in peripheral blood mononuclear cells (in vitro study). Food Chem Toxicol. 84:79–88. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Zhou C, Wang W, Peretz J and Flaws JA: Bisphenol A exposure inhibits germ cell nest breakdown by reducing apoptosis in cultured neonatal mouse ovaries. Reprod Toxicol. 57:87–99. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Iida H, Maehara K, Doiguchi M, Mōri T and Yamada F: Bisphenol A-induced apoptosis of cultured rat Sertoli cells. Reprod Toxicol. 17:457–464. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Kuiper GG, Lemmen JG, Carlsson B, Corton JC, Safe SH, van der Saag PT, van der Burg B and Gustafsson JK: Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor β. Endocrinology. 139:4252–4263. 1998. View Article : Google Scholar : PubMed/NCBI

23 

Omoto Y, Imamov O, Warner M and Gustafsson JA: Estrogen receptor alpha and imprinting of the neonatal mouse ventral prostate by estrogen. Proc Natl Acad Sci USA. 102:pp. 1484–1489. 2005; View Article : Google Scholar : PubMed/NCBI

24 

Adams JY, Leav I, Lau KM, Ho SM and Pfluege SM: Expression of estrogen receptor beta in the fetal, neonatal, and prepubertal human prostate. Prostate. 52:69–81. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Taylor JA, Richter CA, Ruhlen RL and vom Saal FS: Estrogenic environmental chemicals and drugs: Mechanisms for effects on the developing male urogenital system. J Steroid Biochem Mol Biol. 127:83–95. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Vandenberg LN, Maffini MV, Sonnenschein C, Rubin BS and Soto AM: Bisphenol A and the great divide: A review of controversies in the field of endocrine disruption. Endocr Rev. 30:75–95. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Weng YI, Hsu PY, Liyanarachchi S, Liu J, Deatherage DE, Huang YW, Zuo T, Rodriguez B, Lin CH, Cheng AL and Huang TH: Epigenetic influences of low-dose bisphenol A in primary human breast epithelial cells. Toxicol Appl Pharmacol. 248:111–121. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Rubin BS: Bisphenol A: An endocrine disruptor with widespread exposure and multiple effects. J Steroid Biochem Mol Biol. 127:27–34. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Luccio-Camelo DC and Prins GS: Disruption of androgen receptor signaling in males by environmental chemicals. J Steroid Biochem Mol Biol. 127:74–82. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Bonefeld-Jørgensen EC, Long M, Hofmeister MV and Vinggaard AM: Endocrine-disrupting potential of bisphenol A, bisphenol A dimethacrylate, 4-nnonylphenol, and 4-n-octylphenol in vitro: New data and a brief review. Environ Health Perspect. 115 Suppl 1:S69–S76. 2007. View Article : Google Scholar

31 

Wetherill YB, Fisher NL, Staubach A, Danielsen M, de Vere White RW and Knudsen KE: Xenoestrogen action in prostate cancer: Pleiotropic effects dependent on androgen receptor status. Cancer Res. 65:54–65. 2005.PubMed/NCBI

32 

Wetherill YB, Petre CE, Monk KR, Puga A and Knudsen KE: The xenoestrogen bisphenol A induces inappropriate androgen receptor activation and mitogenesis in prostatic adenocarcinoma cells. Mol Cancer Ther. 1:515–524. 2002.PubMed/NCBI

33 

Lee CH, Akin-Olugbade O and Kirschenbaum A: Overview of prostate anatomy, histology, and pathology. Endocrinol Metab Clin North Am. 40565–575. (viii-xi)2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Huang D, Wu J, Su X, Yan H and Sun Z: Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents. Oncol Lett 14: 2635-2642, 2017.
APA
Huang, D., Wu, J., Su, X., Yan, H., & Sun, Z. (2017). Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents. Oncology Letters, 14, 2635-2642. https://doi.org/10.3892/ol.2017.6469
MLA
Huang, D., Wu, J., Su, X., Yan, H., Sun, Z."Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents". Oncology Letters 14.3 (2017): 2635-2642.
Chicago
Huang, D., Wu, J., Su, X., Yan, H., Sun, Z."Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents". Oncology Letters 14, no. 3 (2017): 2635-2642. https://doi.org/10.3892/ol.2017.6469
Copy and paste a formatted citation
x
Spandidos Publications style
Huang D, Wu J, Su X, Yan H and Sun Z: Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents. Oncol Lett 14: 2635-2642, 2017.
APA
Huang, D., Wu, J., Su, X., Yan, H., & Sun, Z. (2017). Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents. Oncology Letters, 14, 2635-2642. https://doi.org/10.3892/ol.2017.6469
MLA
Huang, D., Wu, J., Su, X., Yan, H., Sun, Z."Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents". Oncology Letters 14.3 (2017): 2635-2642.
Chicago
Huang, D., Wu, J., Su, X., Yan, H., Sun, Z."Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents". Oncology Letters 14, no. 3 (2017): 2635-2642. https://doi.org/10.3892/ol.2017.6469
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