Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells

Matsuo,Toshihiro; Sato,Keiji; Matsui,Takuya; Sawada,Shigeyuki; Muramatsu,Yoshitaka; Kawanami,Katsuhisa; Deie,Masataka

doi:10.3892/ol.2017.6472

Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells

Authors:
- Toshihiro Matsuo
- Keiji Sato
- Takuya Matsui
- Shigeyuki Sawada
- Yoshitaka Muramatsu
- Katsuhisa Kawanami
- Masataka Deie
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Affiliations: Department of Orthopaedic Surgery, Aichi Medical University, Nagakute, Aichi 480-1195, Japan, Department of Physiology, Aichi Medical University, Nagakute, Aichi 480-1195, Japan
Published online on: June 23, 2017 https://doi.org/10.3892/ol.2017.6472
Pages: 3071-3076

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Abstract

To date, there is limited data on the biological effects of low‑intensity pulsed ultrasound (LIPUS) on primary malignant bone tumors. The purpose of the present study was to investigate the antitumor effects of LIPUS on osteosarcoma cells. The effects of LIPUS on cell viability, induction of apoptosis, mitochondrial membrane potential and intracellular signaling molecules in the LM8 osteosarcoma cell line were investigated. LIPUS inhibited cell viability (P=0.0022) and mitochondrial membrane potential (P=0.0019) in LM8 cells. Flow cytometry analysis and terminal deoxynucleotidyl transferase dUTP nick end labeling staining revealed significantly higher numbers of apoptotic (P<0.0001) and necrotic cells (P=0.0091) compared with cells without treatment. LIPUS treatment significantly increased phosphorylated Akt (P<0.0001) and IκBα (P=0.0001) levels, and reduced phosphorylated mitogen‑activated protein kinase 7 (P<0.0001) and phosphorylated checkpoint kinase 1 (P=0.0008) levels. These results suggest that LIPUS is a non‑invasive adjuvant therapy that is able to inhibit cellular proliferation in osteosarcoma cells.

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