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Article

Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells

  • Authors:
    • Toshihiro Matsuo
    • Keiji Sato
    • Takuya Matsui
    • Shigeyuki Sawada
    • Yoshitaka Muramatsu
    • Katsuhisa Kawanami
    • Masataka Deie
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Aichi Medical University, Nagakute, Aichi 480-1195, Japan, Department of Physiology, Aichi Medical University, Nagakute, Aichi 480-1195, Japan
  • Pages: 3071-3076
    |
    Published online on: June 23, 2017
       https://doi.org/10.3892/ol.2017.6472
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Abstract

To date, there is limited data on the biological effects of low‑intensity pulsed ultrasound (LIPUS) on primary malignant bone tumors. The purpose of the present study was to investigate the antitumor effects of LIPUS on osteosarcoma cells. The effects of LIPUS on cell viability, induction of apoptosis, mitochondrial membrane potential and intracellular signaling molecules in the LM8 osteosarcoma cell line were investigated. LIPUS inhibited cell viability (P=0.0022) and mitochondrial membrane potential (P=0.0019) in LM8 cells. Flow cytometry analysis and terminal deoxynucleotidyl transferase dUTP nick end labeling staining revealed significantly higher numbers of apoptotic (P<0.0001) and necrotic cells (P=0.0091) compared with cells without treatment. LIPUS treatment significantly increased phosphorylated Akt (P<0.0001) and IκBα (P=0.0001) levels, and reduced phosphorylated mitogen‑activated protein kinase 7 (P<0.0001) and phosphorylated checkpoint kinase 1 (P=0.0008) levels. These results suggest that LIPUS is a non‑invasive adjuvant therapy that is able to inhibit cellular proliferation in osteosarcoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Matsuo T, Sato K, Matsui T, Sawada S, Muramatsu Y, Kawanami K and Deie M: Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells. Oncol Lett 14: 3071-3076, 2017.
APA
Matsuo, T., Sato, K., Matsui, T., Sawada, S., Muramatsu, Y., Kawanami, K., & Deie, M. (2017). Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells. Oncology Letters, 14, 3071-3076. https://doi.org/10.3892/ol.2017.6472
MLA
Matsuo, T., Sato, K., Matsui, T., Sawada, S., Muramatsu, Y., Kawanami, K., Deie, M."Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells". Oncology Letters 14.3 (2017): 3071-3076.
Chicago
Matsuo, T., Sato, K., Matsui, T., Sawada, S., Muramatsu, Y., Kawanami, K., Deie, M."Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells". Oncology Letters 14, no. 3 (2017): 3071-3076. https://doi.org/10.3892/ol.2017.6472
Copy and paste a formatted citation
x
Spandidos Publications style
Matsuo T, Sato K, Matsui T, Sawada S, Muramatsu Y, Kawanami K and Deie M: Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells. Oncol Lett 14: 3071-3076, 2017.
APA
Matsuo, T., Sato, K., Matsui, T., Sawada, S., Muramatsu, Y., Kawanami, K., & Deie, M. (2017). Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells. Oncology Letters, 14, 3071-3076. https://doi.org/10.3892/ol.2017.6472
MLA
Matsuo, T., Sato, K., Matsui, T., Sawada, S., Muramatsu, Y., Kawanami, K., Deie, M."Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells". Oncology Letters 14.3 (2017): 3071-3076.
Chicago
Matsuo, T., Sato, K., Matsui, T., Sawada, S., Muramatsu, Y., Kawanami, K., Deie, M."Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells". Oncology Letters 14, no. 3 (2017): 3071-3076. https://doi.org/10.3892/ol.2017.6472
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