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Article Open Access

Isolation and characterization of adult mammary stem cells from breast cancer‑adjacent tissues

  • Authors:
    • Ai‑Ping Shi
    • Zhi‑Min Fan
    • Ke‑Wei Ma
    • Yan‑Fang Jiang
    • Lei Wang
    • Ke‑Wei Zhang
    • Shi‑Bo Fu
    • Ning Xu
    • Zhi‑Ru Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Oncology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Central Laboratory, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, MH Radiobiology Research Unit, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Urology, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2894-2902
    |
    Published online on: June 28, 2017
       https://doi.org/10.3892/ol.2017.6485
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Abstract

Normal adult mammary stem cells (AMSCs) are promising sources for breast reconstruction, particularly following the resection of breast tumors. However, carcinogenic events can potentially convert normal AMSCs to cancer stem cells, posing a safety concern for the use of AMSCs for clinical tissue regeneration. In the present study, AMSCs and autologous primary breast cancer cells were isolated and compared for their ability to differentiate, their gene expression profile, and their potential to form tumors in vivo. AMSCs were isolated from normal tissue surrounding primary breast tumors by immunomagnetic sorting. The pluripotency of these cells was investigated by differentiation analysis, and gene expression profiles were compared with microarrays. Differentially expressed candidate genes were confirmed by reverse transcription‑polymerase chain reaction and western blot analyses. The in vivo tumorigenicity of these cells, compared with low‑malignancy MCF‑7 cells, was also investigated by xenograft tumor formation analysis. The results revealed that AMSCs isolated from normal tissues surrounding primary breast tumors were positive for the stem cell markers epithelial‑specific antigen and keratin‑19. When stimulated with basic fibroblast growth factor, a differentiation agent, these AMSCs formed lobuloalveolar structures with myoepithelia that were positive for common acute lymphoblastic leukemia antigen. The gene expression profiles revealed that, compared with cancer cells, AMSCs expressed low levels of oncogenes, including MYC, RAS and ErbB receptor tyrosine kinase 2, and high levels of tumor suppressor genes, including RB transcriptional corepressor 1, phosphatase and tensin homolog, and cyclin‑dependent kinase inhibitor 2A. When injected into nude non‑obese diabetic/severe combined immunodeficiency‑type mice, the AMSCs did not form tumors, and regular mammary ductal structures were generated. The AMSCs isolated from normal tissue adjacent to primary breast tumors had the normal phenotype of mammary stem cells, and therefore may be promising candidates for mammary reconstruction subsequent to breast tumor resection.
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1 

Thomas-MacLean R: Beyond dichotomies of health and illness: Life after breast cancer. Nurs Inq. 12:200–209. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Roje Z, Roje Z, Janković S and Ninković M: Breast reconstruction after mastectomy. Coll Antropol. 34 Suppl 1:S113–S123. 2010.

3 

Rozen WM, Rajkomar AK, Anavekar NS and Ashton MW: Post-mastectomy breast reconstruction: A history in evolution. Clin Breast Cancer. 9:145–154. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Djohan R, Gage E and Bernard S: Breast reconstruction options following mastectomy. Cleve Clin J Med. 75 Suppl 1:S17–S23. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Techanukul T and Lohsiriwat V: Stem cell and tissue engineering in breast reconstruction. Gland Surg. 3:55–61. 2014.PubMed/NCBI

6 

Holland MS and Holland RE: The cellular perspective on mammary gland development: Stem/progenitor cells and beyond. J Dairy Sci. 88 Suppl 1:E1–E8. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Deome KB, Faulkin LJ Jr, Bern HA and Blair PB: Development of mammary tumors from hyperplastic alveolar nodules transplanted into gland-free mammary fat pads of female C3H mice. Cancer Res. 19:515–520. 1959.PubMed/NCBI

8 

Ercan C, van Diest PJ and Vooijs M: Mammary development and breast cancer: The role of stem cells. Curr Mol Med. 11:270–285. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Gupta PB, Chaffer CL and Weinberg RA: Cancer stem cells: Mirage or reality? Nat Med. 15:1010–1012. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Li L and Neaves WB: Normal stem cells and cancer stem cells: The niche matters. Cancer Res. 66:4553–4557. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Chen X, Liu Q and Song E: Mammary stem cells: Angels or demons in mammary gland? Signal Transduction Targeted Therapy. 2:160382017. View Article : Google Scholar

12 

Kao CY, Nomata K, Oakley CS, Welsch CW and Chang CC: Two types of normal human breast epithelial cells derived from reduction mammoplasty: Phenotypic characterization and response to SV40 transfection. Carcinogenesis. 16:531–538. 1995. View Article : Google Scholar : PubMed/NCBI

13 

Ponti D, Zaffaroni N, Capelli C and Daidone MG: Breast cancer stem cells: An overview. Eur J Cancer. 42:1219–1224. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Shackleton M, Vaillant F, Simpson KJ, Stingl J, Smyth GK, Asselin-Labat ML, Wu L, Lindeman GJ and Visvader JE: Generation of a functional mammary gland from a single stem cell. Nature. 439:84–88. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Stingl J, Eirew P, Ricketson I, Shackleton M, Vaillant F, Choi D, Li HI and Eaves CJ: Purification and unique properties of mammary epithelial stem cells. Nature. 439:993–997. 2006.PubMed/NCBI

16 

Proia DA and Kuperwasser C: Reconstruction of human mammary tissues in a mouse model. Nat Protoc. 1:206–214. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Kalirai H and Clarke RB: Human breast epithelial stem cells and their regulation. J Pathol. 208:7–16. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Gudjonsson T, Villadsen R, Nielsen HL, Ronnov-Jessen L, Bissell MJ and Petersen OW: Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties. Genes Dev. 16:693–706. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Slaughter DP, Southwick HW and Smejkal W: Field cancerization in oral stratified squamous epithelium; clinical implications of multicentric origin. Cancer. 6:963–968. 1953. View Article : Google Scholar : PubMed/NCBI

20 

Försti A, Louhelainen J, Söderberg M, Wijkström H and Hemminki K: Loss of heterozygosity in tumour-adjacent normal tissue of breast and bladder cancer. Eur J Cancer. 37:1372–1380. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Klevebring D, Rosin G, Ma R, Lindberg J, Czene K, Kere J, Fredriksson I, Bergh J and Hartman J: Sequencing of breast cancer stem cell populations indicates a dynamic conversion between differentiation states in vivo. Breast Cancer Res. 16:R722014. View Article : Google Scholar : PubMed/NCBI

22 

Morroni M, Giordano A, Zingaretti MC, Boiani R, De Matteis R, Kahn BB, Nisoli E, Tonello C, Pisoschi C, Luchetti MM, et al: Reversible transdifferentiation of secretory epithelial cells into adipocytes in the mammary gland. Proc Natl Acad Sci USA. 101:pp. 16801–16806. 2004; View Article : Google Scholar : PubMed/NCBI

23 

De Matteis R, Zingaretti MC, Murano I, Vitali A, Frontini A, Giannulis I, Barbatelli G, Marcucci F, Bordicchia M, Sarzani R, et al: In vivo physiological transdifferentiation of adult adipose cells. Stem Cells. 27:2761–2768. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Mammary stem cells and the differentiation hierarchy: Current status and perspectives. Genes Dev. 28:1143–1158. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Burkhart DL and Sage J: Cellular mechanisms of tumour suppression by the retinoblastoma gene. Nat Rev Cancer. 8:671–682. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Blanco-Aparicio C, Renner O, Leal JF and Carnero A: PTEN, more than the AKT pathway. Carcinogenesis. 28:1379–1386. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Luthy IA, Bruzzone A, Piñero CP, Castillo LF, Chiesa IJ, Vázquez SM and Sarappa MG: Adrenoceptors: Non conventional target for breast cancer? Curr Med Chem. 16:1850–1862. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Caldon CE, Daly RJ, Sutherland RL and Musgrove EA: Cell cycle control in breast cancer cells. J Cell Biochem. 97:261–274. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Walia V, Ding M, Kumar S, Nie D, Premkumar LS and Elble RC: hCLCA2 Is a p53-Inducible Inhibitor of Breast Cancer Cell Proliferation. Cancer Res. 69:6624–6632. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Hitchler MJ, Oberley LW and Domann FE: Epigenetic silencing of SOD2 by histone modifications in human breast cancer cells. Free Radic Biol Med. 45:1573–1580. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Pratt MA, Tibbo E, Robertson SJ, Jansson D, Hurst K, Perez-Iratxeta C, Lau R and Niu MY: The canonical NF-kappaB pathway is required for formation of luminal mammary neoplasias and is activated in the mammary progenitor population. Oncogene. 28:2710–2722. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Margheri F, Serrati S, Lapucci A, Anastasia C, Giusti B, Pucci M, Torre E, Bianchini F, Calorini L, Albini A, et al: Systemic sclerosis-endothelial cell antiangiogenic pentraxin 3 and matrix metalloprotease 12 control human breast cancer tumor vascularization and development in mice. Neoplasia. 11:1106–1115. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Pontier SM and Muller WJ: Integrins in mammary-stem-cell biology and breast-cancer progression-a role in cancer stem cells? J Cell Sci. 122:207–214. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Jin L, Yuan RQ, Fuchs A, Yao Y, Joseph A, Schwall R, Schnitt SJ, Guida A, Hastings HM, Andres J, et al: Expression of interleukin-1beta in human breast carcinoma. Cancer. 80:421–434. 1997. View Article : Google Scholar : PubMed/NCBI

35 

Freudenberg JA, Wang Q, Katsumata M, Drebin J, Nagatomo I and Greene MI: The role of HER2 in early breast cancer metastasis and the origins of resistance to HER2-targeted therapies. Exp Mol Pathol. 87:1–11. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Moon A: Differential functions of Ras for malignant phenotypic conversion. Arch Pharm Res. 29:113–122. 2006. View Article : Google Scholar : PubMed/NCBI

37 

Jamerson MH, Johnson MD and Dickson RB: Of mice and Myc: c-Myc and mammary tumorigenesis. J Mammary Gland Biol Neoplasia. 9:27–37. 2004. View Article : Google Scholar : PubMed/NCBI

38 

Stoelzle T, Schwarb P, Trumpp A and Hynes NE: c-Myc affects mRNA translation, cell proliferation and progenitor cell function in the mammary gland. BMC Biol. 7:632009. View Article : Google Scholar : PubMed/NCBI

39 

van Garderen E and Schalken JA: Morphogenic and tumorigenic potentials of the mammary growth hormone/growth hormone receptor system. Mol Cell Endocrinol. 197:153–165. 2002. View Article : Google Scholar : PubMed/NCBI

40 

Lottin S, Adriaenssens E, Dupressoir T, Berteaux N, Montpellier C, Coll J, Dugimont T and Curgy JJ: Overexpression of an ectopic H19 gene enhances the tumorigenic properties of breast cancer cells. Carcinogenesis. 23:1885–1895. 2002. View Article : Google Scholar : PubMed/NCBI

41 

Hirohata S, Wang LW, Miyagi M, Yan L, Seldin MF, Keene DR, Crabb JW and Apte SS: Punctin, a novel ADAMTS-like molecule, ADAMTSL-1, in extracellular matrix. J Biol Chem. 277:12182–12189. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shi AP, Fan ZM, Ma KW, Jiang YF, Wang L, Zhang KW, Fu SB, Xu N and Zhang ZR: Isolation and characterization of adult mammary stem cells from breast cancer‑adjacent tissues. Oncol Lett 14: 2894-2902, 2017.
APA
Shi, A., Fan, Z., Ma, K., Jiang, Y., Wang, L., Zhang, K. ... Zhang, Z. (2017). Isolation and characterization of adult mammary stem cells from breast cancer‑adjacent tissues. Oncology Letters, 14, 2894-2902. https://doi.org/10.3892/ol.2017.6485
MLA
Shi, A., Fan, Z., Ma, K., Jiang, Y., Wang, L., Zhang, K., Fu, S., Xu, N., Zhang, Z."Isolation and characterization of adult mammary stem cells from breast cancer‑adjacent tissues". Oncology Letters 14.3 (2017): 2894-2902.
Chicago
Shi, A., Fan, Z., Ma, K., Jiang, Y., Wang, L., Zhang, K., Fu, S., Xu, N., Zhang, Z."Isolation and characterization of adult mammary stem cells from breast cancer‑adjacent tissues". Oncology Letters 14, no. 3 (2017): 2894-2902. https://doi.org/10.3892/ol.2017.6485
Copy and paste a formatted citation
x
Spandidos Publications style
Shi AP, Fan ZM, Ma KW, Jiang YF, Wang L, Zhang KW, Fu SB, Xu N and Zhang ZR: Isolation and characterization of adult mammary stem cells from breast cancer‑adjacent tissues. Oncol Lett 14: 2894-2902, 2017.
APA
Shi, A., Fan, Z., Ma, K., Jiang, Y., Wang, L., Zhang, K. ... Zhang, Z. (2017). Isolation and characterization of adult mammary stem cells from breast cancer‑adjacent tissues. Oncology Letters, 14, 2894-2902. https://doi.org/10.3892/ol.2017.6485
MLA
Shi, A., Fan, Z., Ma, K., Jiang, Y., Wang, L., Zhang, K., Fu, S., Xu, N., Zhang, Z."Isolation and characterization of adult mammary stem cells from breast cancer‑adjacent tissues". Oncology Letters 14.3 (2017): 2894-2902.
Chicago
Shi, A., Fan, Z., Ma, K., Jiang, Y., Wang, L., Zhang, K., Fu, S., Xu, N., Zhang, Z."Isolation and characterization of adult mammary stem cells from breast cancer‑adjacent tissues". Oncology Letters 14, no. 3 (2017): 2894-2902. https://doi.org/10.3892/ol.2017.6485
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