Open Access

Long non‑coding RNA Igf2as controls hepatocellular carcinoma progression through the ERK/MAPK signaling pathway

  • Authors:
    • Han Bao
    • Chun‑Guang Guo
    • Peng‑Cheng Qiu
    • Xin‑Lei Zhang
    • Qi Dong
    • Yu‑Kun Wang
  • View Affiliations

  • Published online on: June 30, 2017     https://doi.org/10.3892/ol.2017.6492
  • Pages: 2831-2837
  • Copyright: © Bao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNAs (lncRNAs) serve an important role in numerous human diseases, including cancer. Abnormal expression of lncRNAs has been associated with a number of tumor types; however, the underlying mechanisms through which lncRNA functions have yet to be elucidated. The present study primarily focuses on insulin‑like growth factor 2 antisense 1 (Igf2as), a lncRNA reported to be differentially expressed in hepatocellular carcinoma (HCC). Reverse transcription‑quantitative polymerase chain reaction analysis was used to determine the level of Igf2as in HCC cells and tissues. Flow cytometry was used to determine the level of cell apoptosis following Igf2as suppression and western blot analysis was used to identify altered protein expression levels. The results demonstrated that Igf2as was upregulated in HCC cells and tissues, and that the inhibition of Igf2as using a targeted small interfering RNA (si‑Igf2as), significantly decreased cell proliferation and increased apoptosis. Western blot analysis identified that the extracellular signal‑regulated kinase/mitogen‑activated protein kinase (ERK/MAPK) signaling pathway was inhibited in cells transfected with si‑Igf2as. In addition, cell migration was markedly reduced by the knockdown of Igf2as. These results suggest that lncRNA Igf2as may control hepatocellular progression primarily through the regulation of the ERK/MAPK signaling pathway.
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September-2017
Volume 14 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Bao H, Guo CG, Qiu PC, Zhang XL, Dong Q and Wang YK: Long non‑coding RNA Igf2as controls hepatocellular carcinoma progression through the ERK/MAPK signaling pathway. Oncol Lett 14: 2831-2837, 2017
APA
Bao, H., Guo, C., Qiu, P., Zhang, X., Dong, Q., & Wang, Y. (2017). Long non‑coding RNA Igf2as controls hepatocellular carcinoma progression through the ERK/MAPK signaling pathway. Oncology Letters, 14, 2831-2837. https://doi.org/10.3892/ol.2017.6492
MLA
Bao, H., Guo, C., Qiu, P., Zhang, X., Dong, Q., Wang, Y."Long non‑coding RNA Igf2as controls hepatocellular carcinoma progression through the ERK/MAPK signaling pathway". Oncology Letters 14.3 (2017): 2831-2837.
Chicago
Bao, H., Guo, C., Qiu, P., Zhang, X., Dong, Q., Wang, Y."Long non‑coding RNA Igf2as controls hepatocellular carcinoma progression through the ERK/MAPK signaling pathway". Oncology Letters 14, no. 3 (2017): 2831-2837. https://doi.org/10.3892/ol.2017.6492