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Article

microRNA‑494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer

  • Authors:
    • Aijun Yang
    • Xuenan Wang
    • Chunna Yu
    • Zhenzhen Jin
    • Lingxia Wei
    • Jinghe Cao
    • Qin Wang
    • Min Zhang
    • Lin Zhang
    • Lei Zhang
    • Cuifang Hao
  • View Affiliations / Copyright

    Affiliations: Center for Reproductive Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, P.R. China, Department of Clinical Medicine, Jining Medical University, Jining, Shandong 272067, P.R. China, School of Life Science, Shandong Normal University, Jinan, Shandong 250014, P.R. China, Department of Gynecology and Obstetrics, Qing Dao University, Qingdao, Shandong 266071, P.R. China
  • Pages: 3177-3184
    |
    Published online on: June 30, 2017
       https://doi.org/10.3892/ol.2017.6501
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Abstract

Ovarian cancer is one of the most common types of gynecological malignancy worldwide, and is the fourth leading cause of cancer‑associated mortality among women. Despite improvements in therapeutic treatments, the prognosis for epithelial ovarian cancer (EOC) remains poor, mainly due to the rapid growth and metastasis of ovarian cancer tumors. An increasing number of studies have indicated that microRNAs (miRNAs) are involved in the carcinogenesis and progression of human cancer, suggesting that miRNAs may be used in clinical prognosis and as a therapeutic target in EOC. The aim of the present study was to investigate the expression levels of miRNA‑494 in EOC tissues and cell lines. The clinical significance of miRNA‑494 in patients with EOC was also evaluated. The results demonstrated that miRNA‑494 was significantly downregulated in EOC tissues and cell lines. Low expression levels of miRNA‑494 were associated with poor prognostic features, including International Federation of Gynecology and Obstetrics stage, tumor size and lymph node metastasis. In vitro functional studies demonstrated that overexpression of miRNA‑494 inhibited proliferation, migration and invasion in EOC cells. By contrast, knockdown of miRNA‑494 enhanced cell growth, migration and invasion in EOC cells. Notably, sirtuin 1 (SIRT1) was identified as a direct target of miRNA‑494 in EOC. Furthermore, MTT, cell migration and invasion assays verified that EOC cell proliferation, migration and invasion were completely restored with forced miRNA‑494 expression and SIRT1 restoration. Together, these findings suggest that miRNA‑494 is a potential prognostic marker, and may provide novel therapeutic regimens of targeted therapy for EOC.
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Copy and paste a formatted citation
Spandidos Publications style
Yang A, Wang X, Yu C, Jin Z, Wei L, Cao J, Wang Q, Zhang M, Zhang L, Zhang L, Zhang L, et al: microRNA‑494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer. Oncol Lett 14: 3177-3184, 2017.
APA
Yang, A., Wang, X., Yu, C., Jin, Z., Wei, L., Cao, J. ... Hao, C. (2017). microRNA‑494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer. Oncology Letters, 14, 3177-3184. https://doi.org/10.3892/ol.2017.6501
MLA
Yang, A., Wang, X., Yu, C., Jin, Z., Wei, L., Cao, J., Wang, Q., Zhang, M., Zhang, L., Zhang, L., Hao, C."microRNA‑494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer". Oncology Letters 14.3 (2017): 3177-3184.
Chicago
Yang, A., Wang, X., Yu, C., Jin, Z., Wei, L., Cao, J., Wang, Q., Zhang, M., Zhang, L., Zhang, L., Hao, C."microRNA‑494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer". Oncology Letters 14, no. 3 (2017): 3177-3184. https://doi.org/10.3892/ol.2017.6501
Copy and paste a formatted citation
x
Spandidos Publications style
Yang A, Wang X, Yu C, Jin Z, Wei L, Cao J, Wang Q, Zhang M, Zhang L, Zhang L, Zhang L, et al: microRNA‑494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer. Oncol Lett 14: 3177-3184, 2017.
APA
Yang, A., Wang, X., Yu, C., Jin, Z., Wei, L., Cao, J. ... Hao, C. (2017). microRNA‑494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer. Oncology Letters, 14, 3177-3184. https://doi.org/10.3892/ol.2017.6501
MLA
Yang, A., Wang, X., Yu, C., Jin, Z., Wei, L., Cao, J., Wang, Q., Zhang, M., Zhang, L., Zhang, L., Hao, C."microRNA‑494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer". Oncology Letters 14.3 (2017): 3177-3184.
Chicago
Yang, A., Wang, X., Yu, C., Jin, Z., Wei, L., Cao, J., Wang, Q., Zhang, M., Zhang, L., Zhang, L., Hao, C."microRNA‑494 is a potential prognostic marker and inhibits cellular proliferation, migration and invasion by targeting SIRT1 in epithelial ovarian cancer". Oncology Letters 14, no. 3 (2017): 3177-3184. https://doi.org/10.3892/ol.2017.6501
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