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Article

Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo

  • Authors:
    • Jian Bai
    • Xue Zhang
    • Bangqing Liu
    • Haiyong Wang
    • Zhenzong Du
    • Jianfei Song
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710000, P.R. China, Department of Respiratory Medicine, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710000, P.R. China, Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital of Guilin Medical University, Guilin, Guangxi Zhuang 541100, P.R. China, Department of Thoracic and Cardiovascular Surgery, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi Zhuang 541001, P.R. China, Department of Thoracic Surgery, Nanxi Shan Hospital of Guangxi Zhuang Autonomous Region, Guilin, Guangxi Zhuang 541002, P.R. China
  • Pages: 3077-3081
    |
    Published online on: July 7, 2017
       https://doi.org/10.3892/ol.2017.6535
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Abstract

Previous studies have demonstrated that DNA methyltransferase 1 (DNMT1) is required for the maintenance of DNA methylation and epigenetic changes that may lead to the development of esophageal squamous cell carcinoma (ESCC). In order to investigate whether the silencing of DNMT1 protects tumor suppressor genes, including p16, and is able to be used as a potential therapy for human ESCC, short hairpin RNA targeting DNMT1 (shRNA‑DNMT1) was synthesized and transfected into the human ESCC lines KYSE150 and KYSE410, which were then injected into the backs of nude mice prior to harvesting. Results from the reverse transcription‑quantitative polymerase chain reaction (PCR) and western blotting demonstrated that p16 mRNA expression was increased in the shRNA‑DNMT1‑transfected ESCC cell lines in vitro and in vivo. Consistent with the immunohistochemistry results, p16 was expressed in tumor tissue from nude mice that had been transplanted with the modified human ESCC lines. It was also observed that p16 methylation was inhibited following transfection with shRNA‑DNMT1 as detected using methylation‑specific PCR analysis. The results of the present study suggest that silencing DNMT1 serves a protective role through the demethylation and subsequent activation of p16 in vitro and in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Bai J, Zhang X, Liu B, Wang H, Du Z and Song J: Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo. Oncol Lett 14: 3077-3081, 2017.
APA
Bai, J., Zhang, X., Liu, B., Wang, H., Du, Z., & Song, J. (2017). Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo. Oncology Letters, 14, 3077-3081. https://doi.org/10.3892/ol.2017.6535
MLA
Bai, J., Zhang, X., Liu, B., Wang, H., Du, Z., Song, J."Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo". Oncology Letters 14.3 (2017): 3077-3081.
Chicago
Bai, J., Zhang, X., Liu, B., Wang, H., Du, Z., Song, J."Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo". Oncology Letters 14, no. 3 (2017): 3077-3081. https://doi.org/10.3892/ol.2017.6535
Copy and paste a formatted citation
x
Spandidos Publications style
Bai J, Zhang X, Liu B, Wang H, Du Z and Song J: Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo. Oncol Lett 14: 3077-3081, 2017.
APA
Bai, J., Zhang, X., Liu, B., Wang, H., Du, Z., & Song, J. (2017). Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo. Oncology Letters, 14, 3077-3081. https://doi.org/10.3892/ol.2017.6535
MLA
Bai, J., Zhang, X., Liu, B., Wang, H., Du, Z., Song, J."Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo". Oncology Letters 14.3 (2017): 3077-3081.
Chicago
Bai, J., Zhang, X., Liu, B., Wang, H., Du, Z., Song, J."Silencing DNA methyltransferase 1 leads to the activation of the esophageal suppressor gene p16 in vitro and in vivo". Oncology Letters 14, no. 3 (2017): 3077-3081. https://doi.org/10.3892/ol.2017.6535
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