MicroRNA‑154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH Retraction in /10.3892/ol.2023.13673

Qiao,Wenhui; Cao,Nong; Yang,Lei

doi:10.3892/ol.2017.6558

MicroRNA‑154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH

Retraction in: /10.3892/ol.2023.13673

Authors:
- Wenhui Qiao
- Nong Cao
- Lei Yang
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Affiliations: Department of General Surgery, The First Hospital of Lanzhou University, Chengguan, Lanzhou, Gansu 730000, P.R. China
Published online on: July 8, 2017 https://doi.org/10.3892/ol.2017.6558
Pages: 3268-3274

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Abstract

MicroRNAs (miRNAs) are a group of non‑protein‑coding, highly conserved single‑stranded RNA molecules. The abnormal expression of miRNAs has been demonstrated to have an important function in the carcinogenesis and progression of gastric cancer. microRNA‑154 (miR‑154) has been reported to be downregulated in non‑small cell lung, colorectal and prostate cancer. However, the expression and roles of miR‑154 in gastric cancer remain to be established. The present study measured the expression levels of miR‑154 in gastric cancer tissues and cell lines. miR‑154 was found to be significantly downregulated in gastric cancer tissues and cell lines. In addition, functional studies indicated that the overexpression of miR‑154 inhibited the proliferation, migration and invasion of gastric cancer cells. Using TargetScan, a dual luciferase reporter assay, reverse transcription‑quantitative polymerase chain reaction and western blot analysis, metadherin (MTDH) was revealed as a novel miR‑154 target. In addition, knocking down MTDH lead to a similar effect as overexpressing‑154 in gastric cells. The present findings indicate that miR‑154 was downregulated in gastric cancer, and inhibited tumor behaviors of gastric cancer cells partially through the downregulation of MTDH. Therefore, the miR‑154/MTDH axis may be a novel therapeutic to treat patients with gastric cancer.

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