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Article Open Access

Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA‑7 in human hepatocellular carcinoma cells

  • Authors:
    • Yanna Wang
    • Qiaoling Wang
    • Jiqing Song
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Diseases, Yantai Hospital For Infectious Diseases, Yantai, Shandong 264001, P.R. China, Nursing Department of Yantai Yeda Hospital, Yantai, Shandong 264006, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3566-3572
    |
    Published online on: July 15, 2017
       https://doi.org/10.3892/ol.2017.6573
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Abstract

MicroRNAs (miRNAs/miRs) are important molecules that are able to regulate multiple cellular processes in cancer cells. miR‑7 has been previously identified as a tumor suppressive miRNA in several types of cancer. The aim of the present study was to investigate whether miR‑7 is able to regulate autophagy in hepatocellular carcinoma (HCC) cells. It was identified that miR‑7 was significantly downregulated in tumor tissues compared with adjacent normal tissues. Overexpression of miR‑7 inhibited cell proliferative activity, which was partially reversed by miR‑7 inhibitor. In addition, overexpression of miR‑7 significantly induced an increasen in autophagic activity, and luciferase activity assay and western blot analysis identified that mammalian target of rapamycin (mTOR) was a direct target of miR‑7. In addition, inhibition of autophagy by 3‑methyladenine resulted in a marked enhancement of the proliferation inhibition effect of miR‑7. In conclusion, miR‑7 was identified to induce proliferation inhibition and autophagy in HCC cells by targeting mTOR, and inhibition of autophagy may be utilized to enhance the antitumor activity of miR‑7.
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1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Balogh J, Victor D III, Asham EH, Burroughs SG, Boktour M, Saharia A, Li X, Ghobrial RM and Monsour HP Jr: Hepatocellular carcinoma: a review. J Hepatocell Carcinoma. 3:41–53. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Levine B and Kroemer G: Autophagy in the pathogenesis of disease. Cell. 132:27–42. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Mizushima N, Levine B, Cuervo AM and Klionsky DJ: Autophagy fights disease through cellular self-digestion. Nature. 451:1069–1075. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Puri P and Chandra A: Autophagy modulation as a potential therapeutic target for liver diseases. J Clin Exp Hepatol. 4:51–59. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Kumar A, Singh UK and Chaudhary A: Targeting autophagy to overcome drug resistance in cancer therapy. Future Med Chem. 7:1535–1542. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Du H, Yang W, Chen L, Shi M, Seewoo V, Wang J, Lin A, Liu Z and Qiu W: Role of autophagy in resistance to oxaliplatin in hepatocellular carcinoma cells. Oncol Rep. 27:143–150. 2012.PubMed/NCBI

8 

Jung CH, Ro SH, Cao J, Otto NM and Kim DH: mTOR regulation of autophagy. FEBS Lett. 584:1287–1295. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Bupathi M, Kaseb A, Meric-Bernstam F and Naing A: Hepatocellular carcinoma: Where there is unmet need. Mol Oncol. 9:1501–1509. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Wang Z, Jin W, Jin H and Wang X: mTOR in viral hepatitis and hepatocellular carcinoma: Function and treatment. Biomed Res Int. 2014:7356722014.PubMed/NCBI

11 

Inamura K: Diagnostic and therapeutic potential of MicroRNAs in lung cancer. Cancers (Basel). 9:pii: E49. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Cătană CS, Pichler M, Giannelli G, Mader RM and Berindan-Neagoe I: Non-coding RNAs, the Trojan horse in two-way communication between tumor and stroma in colorectal and hepatocellular carcinoma. Oncotarget. 8:29519–29534. 2017.PubMed/NCBI

13 

Faller M and Guo F: MicroRNA biogenesis: There's more than one way to skin a cat. Biochim Biophys Acta. 1779:663–667. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Yao M, Wang L, Yao Y, Gu HB and Yao DF: Biomarker-based MicroRNA therapeutic strategies for hepatocellular carcinoma. J Clin Transl Hepatol. 2:253–258. 2014.PubMed/NCBI

15 

Horsham JL, Kalinowski FC, Epis MR, Ganda C, Brown RA and Leedman PJ: Clinical potential of microRNA-7 in cancer. J Clin Med. 4:1668–1687. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Kalinowski FC, Brown RA, Ganda C, Giles KM, Epis MR, Horsham J and Leedman PJ: microRNA-7: A tumor suppressor miRNA with therapeutic potential. Int J Biochem Cell Biol. 54:312–317. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Zhang X, Hu S, Zhang X, Wang L, Zhang X, Yan B, Zhao J, Yang A and Zhang R: MicroRNA-7 arrests cell cycle in G1 phase by directly targeting CCNE1 in human hepatocellular carcinoma cells. Biochem Biophys Res Commun. 443:1078–1084. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Xiong S, Zheng Y, Jiang P, Liu R, Liu X and Chu Y: MicroRNA-7 inhibits the growth of human non-small cell lung cancer A549 cells through targeting BCL-2. Int J Biol Sci. 7:805–814. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Luo J, Li H and Zhang C: MicroRNA-7 inhibits the malignant phenotypes of non-small cell lung cancer in vitro by targeting Pax6. Mol Med Rep. 12:5443–5448. 2015.PubMed/NCBI

20 

Chang YL, Zhou PJ, Wei L, Li W, Ji Z, Fang YX and Gao WQ: MicroRNA-7 inhibits the stemness of prostate cancer stem-like cells and tumorigenesis by repressing KLF4/PI3K/Akt/p21 pathway. Oncotarget. 6:24017–24031. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Fang Y, Xue JL, Shen Q, Chen J and Tian L: MicroRNA-7 inhibits tumor growth and metastasis by targeting the phosphoinositide 3-kinase/Akt pathway in hepatocellular carcinoma. Hepatology. 55:1852–1862. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Xie J, Chen M, Zhou J, Mo MS, Zhu LH, Liu YP, Gui QJ, Zhang L and Li GQ: miR-7 inhibits the invasion and metastasis of gastric cancer cells by suppressing epidermal growth factor receptor expression. Oncol Rep. 31:1715–1722. 2014.PubMed/NCBI

24 

Bitto A, Lerner CA, Nacarelli T, Crowe E, Torres C and Sell C: P62/SQSTM1 at the interface of aging, autophagy, and disease. Age (Dordr). 36:96262014. View Article : Google Scholar : PubMed/NCBI

25 

Zhu AX: Systemic treatment of hepatocellular carcinoma: Dawn of a new era? Ann Surg Oncol. 17:1247–1256. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Gandellini P, Doldi V and Zaffaroni N: microRNAs as players and signals in the metastatic cascade: Implications for the development of novel anti-metastatic therapies. Semin Cancer Biol. Mar 23–2017.(Epub ahead of Print). View Article : Google Scholar : PubMed/NCBI

27 

Denaro N, Merlano MC, Russi EG and Lo Nigro C: Non coding RNAs in head and neck squamous cell carcinoma (HNSCC): A clinical perspective. Anticancer Res. 34:6887–6896. 2014.PubMed/NCBI

28 

Karagonlar ZF, Korhan P and Atabey N: Targeting c-Met in cancer by MicroRNAs: Potential therapeutic applications in hepatocellular carcinoma. Drug Dev Res. 76:357–367. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Luo T, Fu J, Xu A, Su B, Ren Y, Li N, Zhu J, Zhao X, Dai R, Cao J, et al: PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression. Autophagy. 12:1355–1371. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Tian Y, Kuo CF, Sir D, Wang L, Govindarajan S, Petrovic LM and Ou JH: Autophagy inhibits oxidative stress and tumor suppressors to exert its dual effect on hepatocarcinogenesis. Cell Death Differ. 22:1025–1034. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Huang GM, Jiang QH, Cai C, Qu M and Shen W: SCD1 negatively regulates autophagy-induced cell death in human hepatocellular carcinoma through inactivation of the AMPK signaling pathway. Cancer Lett. 358:180–190. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Wang Y, Wu J, Lin B, Li X, Zhang H, Ding H, Chen X, Lan L and Luo H: Galangin suppresses HepG2 cell proliferation by activating the TGF-β receptor/Smad pathway. Toxicology. 326:9–17. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Lan SH, Wu SY, Zuchini R, Lin XZ, Su IJ, Tsai TF, Lin YJ, Wu CT and Liu HS: Autophagy suppresses tumorigenesis of hepatitis B virus-associated hepatocellular carcinoma through degradation of microRNA-224. Hepatology. 59:505–517. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Gozuacik D, Akkoc Y, Ozturk DG and Kocak M: Autophagy-Regulating microRNAs and cancer. Front Oncol. 7:652017. View Article : Google Scholar : PubMed/NCBI

35 

Liu L, Liao JZ, He XX and Li PY: The role of autophagy in hepatocellular carcinoma: Friend or foe. Oncotarget. Apr 18–2017.(Epub ahead of print).

36 

He C, Dong X, Zhai B, Jiang X, Dong D, Li B, Jiang H, Xu S and Sun X: MiR-21 mediates sorafenib resistance of hepatocellular carcinoma cells by inhibiting autophagy via the PTEN/Akt pathway. Oncotarget. 6:28867–28881. 2015. View Article : Google Scholar : PubMed/NCBI

37 

Tazawa H, Yano S, Yoshida R, Yamasaki Y, Sasaki T, Hashimoto Y, Kuroda S, Ouchi M, Onishi T, Uno F, et al: Genetically engineered oncolytic adenovirus induces autophagic cell death through an E2F1-microRNA-7-epidermal growth factor receptor axis. Int J Cancer. 131:2939–2950. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Wang Q and Song J: Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA‑7 in human hepatocellular carcinoma cells. Oncol Lett 14: 3566-3572, 2017.
APA
Wang, Y., Wang, Q., & Song, J. (2017). Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA‑7 in human hepatocellular carcinoma cells. Oncology Letters, 14, 3566-3572. https://doi.org/10.3892/ol.2017.6573
MLA
Wang, Y., Wang, Q., Song, J."Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA‑7 in human hepatocellular carcinoma cells". Oncology Letters 14.3 (2017): 3566-3572.
Chicago
Wang, Y., Wang, Q., Song, J."Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA‑7 in human hepatocellular carcinoma cells". Oncology Letters 14, no. 3 (2017): 3566-3572. https://doi.org/10.3892/ol.2017.6573
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Wang Q and Song J: Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA‑7 in human hepatocellular carcinoma cells. Oncol Lett 14: 3566-3572, 2017.
APA
Wang, Y., Wang, Q., & Song, J. (2017). Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA‑7 in human hepatocellular carcinoma cells. Oncology Letters, 14, 3566-3572. https://doi.org/10.3892/ol.2017.6573
MLA
Wang, Y., Wang, Q., Song, J."Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA‑7 in human hepatocellular carcinoma cells". Oncology Letters 14.3 (2017): 3566-3572.
Chicago
Wang, Y., Wang, Q., Song, J."Inhibition of autophagy potentiates the proliferation inhibition activity of microRNA‑7 in human hepatocellular carcinoma cells". Oncology Letters 14, no. 3 (2017): 3566-3572. https://doi.org/10.3892/ol.2017.6573
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