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Article Open Access

PCDH8 inhibits glioma cell proliferation by negatively regulating the AKT/GSK3β/β‑catenin signaling pathway

  • Authors:
    • Zhenkun Zong
    • Hui Pang
    • Rutong Yu
    • Yunqi Jiao
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Department of Cardiovascular Medicine, Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical University, Affiliated Xuzhou Hospital of Medical College of Southeast University, Xuzhou, Jiangsu 221009, P.R. China
    Copyright: © Zong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3357-3362
    |
    Published online on: July 20, 2017
       https://doi.org/10.3892/ol.2017.6629
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Abstract

Protocadherin‑8 (PCDH8), a member of the protocadherin superfamily of proteins, is frequently lost in numerous types of cancer. However, the role that PCDH8 serves in human glioma, and the molecular mechanisms underlying this, remain unclear. Data from the present study demonstrated that the expression levels of PCDH8 mRNA and protein were significantly decreased in human glioma tissue compared with normal brain tissue. This suggested that PCDH8 is associated with the development of glioma. Thus, the role of PCDH8 in glioma cell proliferation was investigated by silencing and overexpressing PCDH8 in U251 glioma cells. Overexpression of PCDH8 significantly inhibited glioma cell proliferation, while silencing of PCDH8 using small interfering RNA promoted glioma cell proliferation. Restoration of PCDH8 decreased phosphorylated (p)‑Rac‑α serine/threonine‑protein kinase (AKT) [Threonine (T)308/Serine (S)473] and p‑glycogen synthase kinase‑3β (p‑GSK3β) (S9) protein expression, thereby reducing the level of β‑catenin when compared with the control. By contrast, silencing of PCDH8 increased levels of p‑AKT (T308/S473) and p‑GSK3β (S9), thereby increasing the level of β‑catenin. In conclusion, the results of the present study suggested that PCDH8 suppressed glioma cell proliferation, and that the loss of PCDH8 may stimulate the proto‑oncogene Wnt/β‑catenin signaling pathway and therefore promote glioma cell proliferation.
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Copy and paste a formatted citation
Spandidos Publications style
Zong Z, Pang H, Yu R and Jiao Y: PCDH8 inhibits glioma cell proliferation by negatively regulating the AKT/GSK3β/β‑catenin signaling pathway. Oncol Lett 14: 3357-3362, 2017.
APA
Zong, Z., Pang, H., Yu, R., & Jiao, Y. (2017). PCDH8 inhibits glioma cell proliferation by negatively regulating the AKT/GSK3β/β‑catenin signaling pathway. Oncology Letters, 14, 3357-3362. https://doi.org/10.3892/ol.2017.6629
MLA
Zong, Z., Pang, H., Yu, R., Jiao, Y."PCDH8 inhibits glioma cell proliferation by negatively regulating the AKT/GSK3β/β‑catenin signaling pathway". Oncology Letters 14.3 (2017): 3357-3362.
Chicago
Zong, Z., Pang, H., Yu, R., Jiao, Y."PCDH8 inhibits glioma cell proliferation by negatively regulating the AKT/GSK3β/β‑catenin signaling pathway". Oncology Letters 14, no. 3 (2017): 3357-3362. https://doi.org/10.3892/ol.2017.6629
Copy and paste a formatted citation
x
Spandidos Publications style
Zong Z, Pang H, Yu R and Jiao Y: PCDH8 inhibits glioma cell proliferation by negatively regulating the AKT/GSK3β/β‑catenin signaling pathway. Oncol Lett 14: 3357-3362, 2017.
APA
Zong, Z., Pang, H., Yu, R., & Jiao, Y. (2017). PCDH8 inhibits glioma cell proliferation by negatively regulating the AKT/GSK3β/β‑catenin signaling pathway. Oncology Letters, 14, 3357-3362. https://doi.org/10.3892/ol.2017.6629
MLA
Zong, Z., Pang, H., Yu, R., Jiao, Y."PCDH8 inhibits glioma cell proliferation by negatively regulating the AKT/GSK3β/β‑catenin signaling pathway". Oncology Letters 14.3 (2017): 3357-3362.
Chicago
Zong, Z., Pang, H., Yu, R., Jiao, Y."PCDH8 inhibits glioma cell proliferation by negatively regulating the AKT/GSK3β/β‑catenin signaling pathway". Oncology Letters 14, no. 3 (2017): 3357-3362. https://doi.org/10.3892/ol.2017.6629
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