Open Access

Salvia miltiorrhiza extract inhibits TPA‑induced MMP‑9 expression and invasion through the MAPK/AP‑1 signaling pathway in human breast cancer MCF‑7 cells

  • Authors:
    • Jeong‑Mi Kim
    • Eun‑Mi Noh
    • Hyun‑Kyung Song
    • Minok Lee
    • Soo Ho Lee
    • Sueng Hyuk Park
    • Chan‑Keun Ahn
    • Guem‑San Lee
    • Eui‑Baek Byun
    • Beom‑Su Jang
    • Kang‑Beom Kwon
    • Young‑Rae Lee
  • View Affiliations

  • Published online on: July 20, 2017     https://doi.org/10.3892/ol.2017.6638
  • Pages: 3594-3600
  • Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cancer cell invasion is crucial for metastasis. A major factor in the capacity of cancer cell invasion is the activation of matrix metalloproteinase‑9 (MMP‑9), which degrades the extracellular matrix. Salvia miltiorrhiza has been used as a promotion for blood circulation to remove blood stasis. Numerous previous studies have demonstrated that S. miltiorrhiza extracts (SME) decrease lipid levels and inhibit inflammation. However, the mechanism behind the effect of SME on breast cancer invasion has not been identified. The inhibitory effects of SME on 12‑O‑tetradecanoylphorbol‑13‑acetate (TPA)‑induced MMP‑9 expression were assessed using western blotting, reverse transcription‑quantitative polymerase chain reaction and zymography assays. MMP‑9 upstream signal proteins, including mitogen‑activated protein kinases and activator protein 1 (AP‑1) were also investigated. Cell invasion was assessed using a matrigel invasion assay. The present study demonstrated the inhibitory effects of the SME ethanol solution on MMP‑9 expression and cell invasion in TPA‑treated MCF‑7 breast cancer cells. SME suppressed TPA‑induced MMP‑9 expression and MCF‑7 cell invasion by blocking the transcriptional activation of AP‑1. SME may possess therapeutic potential for inhibiting breast cancer cell invasiveness.
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September-2017
Volume 14 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Kim JM, Noh EM, Song HK, Lee M, Lee S, Park S, Ahn CK, Lee GS, Byun EB, Jang BS, Jang BS, et al: Salvia miltiorrhiza extract inhibits TPA‑induced MMP‑9 expression and invasion through the MAPK/AP‑1 signaling pathway in human breast cancer MCF‑7 cells. Oncol Lett 14: 3594-3600, 2017
APA
Kim, J., Noh, E., Song, H., Lee, M., Lee, S., Park, S. ... Lee, Y. (2017). Salvia miltiorrhiza extract inhibits TPA‑induced MMP‑9 expression and invasion through the MAPK/AP‑1 signaling pathway in human breast cancer MCF‑7 cells. Oncology Letters, 14, 3594-3600. https://doi.org/10.3892/ol.2017.6638
MLA
Kim, J., Noh, E., Song, H., Lee, M., Lee, S., Park, S., Ahn, C., Lee, G., Byun, E., Jang, B., Kwon, K., Lee, Y."Salvia miltiorrhiza extract inhibits TPA‑induced MMP‑9 expression and invasion through the MAPK/AP‑1 signaling pathway in human breast cancer MCF‑7 cells". Oncology Letters 14.3 (2017): 3594-3600.
Chicago
Kim, J., Noh, E., Song, H., Lee, M., Lee, S., Park, S., Ahn, C., Lee, G., Byun, E., Jang, B., Kwon, K., Lee, Y."Salvia miltiorrhiza extract inhibits TPA‑induced MMP‑9 expression and invasion through the MAPK/AP‑1 signaling pathway in human breast cancer MCF‑7 cells". Oncology Letters 14, no. 3 (2017): 3594-3600. https://doi.org/10.3892/ol.2017.6638