Open Access

Association between MDR1 gene of gastrointestinal tumors, the expression of P-glycoprotein and resistance to chemotherapeutic drugs

  • Authors:
    • Jian-Li Su
    • Cheng-Hong Wang
    • Hong-Gang Kang
    • Jing Zhang
    • Bao-Zhong Wang
    • Mei-Rong Liu
    • Jun Zhao
    • Lin Liu
  • View Affiliations

  • Published online on: July 20, 2017     https://doi.org/10.3892/ol.2017.6642
  • Pages: 3510-3514
  • Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to examine and discuss the association between multidrug resistance 1 gene (MDR1) of gastrointestinal tumors, the expression of P-glycoprotein and resistance to chemotherapeutic drugs. In this study, 126 cases of patients with gastrointestinal tumors admitted to hospital from February 2013 to February 2015 were selected. The expression levels of MDR1 gene were obsreved in the control population and patients before and after treatment by fluoresecent quantitative PCR. The protein expression level of P-glycoprotein was determined using western blotting and enzyme-linked immunosorbent assay. In addition, drug resistance was assessed by ATP-TCA chemosensitivity experiments. The results showed that before treatment, the expression of mRNA in MDR1 of tissues of gastrointestinal tract of the 126 cases was 108‑fold larger than that of the gastrointestinal tract of the controls (p<0.05), P-glycoprotein was 87‑fold larger than the expression level of the controls (p<0.05). The sensitivity of 126 tumor tissues to different chemotherapeutic drugs was determined, and the results showed that most of the tumor tissues were sensitive to chemotherapeutic drugs, and the sensitivity rate reached 96.4%. Following chemotherapy, the expression of mRNA in MDR1 of tumor tissues and the expression of P-glycoprotein decreased (p<0.05). In conclusion, the MDR1 gene and P-glycoprotein have a positive correlation with the occurrence of gastrointestinal tumors, and a negative correlation between the MDR1 gene and P-glycoprotein with resistance of chemotherapeutic drugs. Therefore, the MDR1 gene and P-glycoprotein can be used as references in the identification and diagnosis of gastrointestinal tumors.
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September-2017
Volume 14 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Su J, Wang C, Kang H, Zhang J, Wang B, Liu M, Zhao J and Liu L: Association between MDR1 gene of gastrointestinal tumors, the expression of P-glycoprotein and resistance to chemotherapeutic drugs. Oncol Lett 14: 3510-3514, 2017
APA
Su, J., Wang, C., Kang, H., Zhang, J., Wang, B., Liu, M. ... Liu, L. (2017). Association between MDR1 gene of gastrointestinal tumors, the expression of P-glycoprotein and resistance to chemotherapeutic drugs. Oncology Letters, 14, 3510-3514. https://doi.org/10.3892/ol.2017.6642
MLA
Su, J., Wang, C., Kang, H., Zhang, J., Wang, B., Liu, M., Zhao, J., Liu, L."Association between MDR1 gene of gastrointestinal tumors, the expression of P-glycoprotein and resistance to chemotherapeutic drugs". Oncology Letters 14.3 (2017): 3510-3514.
Chicago
Su, J., Wang, C., Kang, H., Zhang, J., Wang, B., Liu, M., Zhao, J., Liu, L."Association between MDR1 gene of gastrointestinal tumors, the expression of P-glycoprotein and resistance to chemotherapeutic drugs". Oncology Letters 14, no. 3 (2017): 3510-3514. https://doi.org/10.3892/ol.2017.6642