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Article

Andrographolide suppresses proliferation of human colon cancer SW620 cells through the TLR4/NF‑κB/MMP‑9 signaling pathway

  • Authors:
    • Rui Zhang
    • Jian Zhao
    • Jian Xu
    • De‑Xin Jiao
    • Jian Wang
    • Zhi‑Qiang Gong
    • Jian‑Hui Jia
  • View Affiliations / Copyright

    Affiliations: Department of Colorectal Surgery, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, P.R. China, Department of Radiotherapy, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, P.R. China
  • Pages: 4305-4310
    |
    Published online on: July 26, 2017
       https://doi.org/10.3892/ol.2017.6669
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Abstract

Modern pharmacological research has revealed that andrographolide has various functions, including anti‑bacterial, anti‑inflammatory and anti‑viral effects, immunoregulation, treating cardiovascular and cerebrovascular diseases, and prevention and treatment of alcoholic liver injury. The present study investigated whether andrographolide suppresses the proliferation of human colon cancer cell through the Toll‑like receptor 4 (TLR4)/nuclear factor (NF)‑κB/matrix metalloproteinase‑9 (MMP‑9) signaling pathway. The MTT assay and lactate dehydrogenase assay were used to evaluate the anticancer effects of andrographolide on cell proliferation and cytotoxicity in human colon cancer SW620 cells. Flow cytometry was used to analyze the anticancer effects of andrographolide on apoptosis by Annexin V‑fluorescein isothiocyanate/propidium iodide kit. The effects of andrographolide on the activity of caspase‑3/9 were measured using ELISA. Western blot analysis was also used to analyze the protein expression of TLR4, myeloid differentiation primary response gene 88 (MyD88), NF‑κB‑p65 and MMP‑9. In the present study, it was found that andrographolide suppressed the cell proliferation, augmented cytotoxicity, evoked cell apoptosis and activated caspase‑3/9 activities in human colon cancer SW620 cells. The results revealed that the anti‑proliferation effects of andrographolide on the SW620 cells was associated with the inhibition of TLR4, MyD88, NF‑κB‑p65 and MMP‑9 signaling activation. The results suggest that andrographolide is a promising drug for treatment of human colon cancer via suppression of the TLR4/NF‑κB/MMP‑9 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang R, Zhao J, Xu J, Jiao DX, Wang J, Gong ZQ and Jia JH: Andrographolide suppresses proliferation of human colon cancer SW620 cells through the TLR4/NF‑κB/MMP‑9 signaling pathway. Oncol Lett 14: 4305-4310, 2017.
APA
Zhang, R., Zhao, J., Xu, J., Jiao, D., Wang, J., Gong, Z., & Jia, J. (2017). Andrographolide suppresses proliferation of human colon cancer SW620 cells through the TLR4/NF‑κB/MMP‑9 signaling pathway. Oncology Letters, 14, 4305-4310. https://doi.org/10.3892/ol.2017.6669
MLA
Zhang, R., Zhao, J., Xu, J., Jiao, D., Wang, J., Gong, Z., Jia, J."Andrographolide suppresses proliferation of human colon cancer SW620 cells through the TLR4/NF‑κB/MMP‑9 signaling pathway". Oncology Letters 14.4 (2017): 4305-4310.
Chicago
Zhang, R., Zhao, J., Xu, J., Jiao, D., Wang, J., Gong, Z., Jia, J."Andrographolide suppresses proliferation of human colon cancer SW620 cells through the TLR4/NF‑κB/MMP‑9 signaling pathway". Oncology Letters 14, no. 4 (2017): 4305-4310. https://doi.org/10.3892/ol.2017.6669
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang R, Zhao J, Xu J, Jiao DX, Wang J, Gong ZQ and Jia JH: Andrographolide suppresses proliferation of human colon cancer SW620 cells through the TLR4/NF‑κB/MMP‑9 signaling pathway. Oncol Lett 14: 4305-4310, 2017.
APA
Zhang, R., Zhao, J., Xu, J., Jiao, D., Wang, J., Gong, Z., & Jia, J. (2017). Andrographolide suppresses proliferation of human colon cancer SW620 cells through the TLR4/NF‑κB/MMP‑9 signaling pathway. Oncology Letters, 14, 4305-4310. https://doi.org/10.3892/ol.2017.6669
MLA
Zhang, R., Zhao, J., Xu, J., Jiao, D., Wang, J., Gong, Z., Jia, J."Andrographolide suppresses proliferation of human colon cancer SW620 cells through the TLR4/NF‑κB/MMP‑9 signaling pathway". Oncology Letters 14.4 (2017): 4305-4310.
Chicago
Zhang, R., Zhao, J., Xu, J., Jiao, D., Wang, J., Gong, Z., Jia, J."Andrographolide suppresses proliferation of human colon cancer SW620 cells through the TLR4/NF‑κB/MMP‑9 signaling pathway". Oncology Letters 14, no. 4 (2017): 4305-4310. https://doi.org/10.3892/ol.2017.6669
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