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Microplate magnetic chemiluminescence immunoassay for detecting urinary survivin in bladder cancer

  • Authors:
    • Yanli Chang
    • Jianjun Xu
    • Qingyun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China
    Copyright: © Chang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4043-4052
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    Published online on: July 26, 2017
       https://doi.org/10.3892/ol.2017.6675
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Abstract

Survivin is a tumor marker for bladder cancer; however the role of urinary survivin levels has not been fully elucidated due to the limitations of current detection methods. Based on two survivin‑specific monoclonal antibodies (McAbs) already confirmed through enzyme linked immunosorbent assays, the present study aimed to establish a microplate magnetic chemiluminescence immunoassay (CLIA) for the detection of urinary survivin levels and evaluate its application for the diagnosis of patients with bladder cancer. Horseradish peroxidase and biotin conjugates were used to label two different anti‑survivin McAbs, respectively. The labeled antibodies combined with survivin to form a sandwiched immune complex. The streptavidin magnetic particles (MPs) served as the solid phase and the separator. The relevant parameters involved in the immunoassay, including the immunoassay reagents used and the physicochemical parameters were optimized. Then, urine samples from 130 patients with bladder cancer and 113 healthy controls were detected, and analyzed using the established method. The method was linear to 1,000 ng/ml survivin with a detection limit of 0.83 ng/ml. The intra‑ and inter‑assay coefficients of variation were <8, and <11%, respectively. The concentration of diluted survivin and the dilution ratios gave a linear correlation of 0.9989. The results demonstrated that the urinary survivin levels in patients with bladder cancer were significantly higher (P<0.001) compared with that in healthy controls. At a survivin concentration of 2.0884 ng/ml, the sensitivity and specificity were 86.9 and 61.9%, respectively. Furthermore, the urinary survivin levels were positively correlated with metastatic stage, histological stage and recurrence (P<0.01). In conclusion, the present study preliminarily proposed a microplate magnetic CLIA for survivin detection and further evaluated the value of urinary survivin as a diagnostic marker for bladder cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Chang Y, Xu J and Zhang Q: Microplate magnetic chemiluminescence immunoassay for detecting urinary survivin in bladder cancer. Oncol Lett 14: 4043-4052, 2017.
APA
Chang, Y., Xu, J., & Zhang, Q. (2017). Microplate magnetic chemiluminescence immunoassay for detecting urinary survivin in bladder cancer. Oncology Letters, 14, 4043-4052. https://doi.org/10.3892/ol.2017.6675
MLA
Chang, Y., Xu, J., Zhang, Q."Microplate magnetic chemiluminescence immunoassay for detecting urinary survivin in bladder cancer". Oncology Letters 14.4 (2017): 4043-4052.
Chicago
Chang, Y., Xu, J., Zhang, Q."Microplate magnetic chemiluminescence immunoassay for detecting urinary survivin in bladder cancer". Oncology Letters 14, no. 4 (2017): 4043-4052. https://doi.org/10.3892/ol.2017.6675
Copy and paste a formatted citation
x
Spandidos Publications style
Chang Y, Xu J and Zhang Q: Microplate magnetic chemiluminescence immunoassay for detecting urinary survivin in bladder cancer. Oncol Lett 14: 4043-4052, 2017.
APA
Chang, Y., Xu, J., & Zhang, Q. (2017). Microplate magnetic chemiluminescence immunoassay for detecting urinary survivin in bladder cancer. Oncology Letters, 14, 4043-4052. https://doi.org/10.3892/ol.2017.6675
MLA
Chang, Y., Xu, J., Zhang, Q."Microplate magnetic chemiluminescence immunoassay for detecting urinary survivin in bladder cancer". Oncology Letters 14.4 (2017): 4043-4052.
Chicago
Chang, Y., Xu, J., Zhang, Q."Microplate magnetic chemiluminescence immunoassay for detecting urinary survivin in bladder cancer". Oncology Letters 14, no. 4 (2017): 4043-4052. https://doi.org/10.3892/ol.2017.6675
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