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Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer

  • Authors:
    • Vanessa Arcolia
    • Fabrice Journe
    • Florence Renaud
    • Emmanuelle Leteurtre
    • Hans‑Joachim Gabius
    • Myriam Remmelink
    • Sven Saussez
  • View Affiliations / Copyright

    Affiliations: Laboratory of Human Anatomy and Experimental Oncology, Faculty of Medicine and Pharmacy, University of Mons, B‑7000 Mons, Belgium, Lille University, UMR‑S 1172, JPARC, Jean‑Pierre Aubert Research Center, F‑59000 Lille, France, Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig‑Maximilians‑University, D‑80539 Munich, Germany, Department of Pathology, Erasme Hospital, Free University of Brussels, B-1070 Brussels, Belgium
    Copyright: © Arcolia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4183-4189
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    Published online on: August 4, 2017
       https://doi.org/10.3892/ol.2017.6719
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Abstract

Currently, fine‑needle aspiration is the most frequently used pre‑operative technique for diagnosis of malignant thyroid tumors, however, pathologists are unable to reach efficient and accurate differential diagnoses between benign and malignant thyroid nodules. To aid in resolving this issue, immunohistochemistry for galectins (gal)‑1, ‑3, ‑7, ‑8, cytokeratin 19 (CK19), Hector Battifora Mesothelial Epitope‑1 (HBME‑1) and thyroid peroxidase (TPO) was performed on two tissue microarrays composed of 66 follicular adenomas (FA) and 66 papillary carcinomas (PC). The identification of optimal cut‑off levels and the diagnostic value of single immunomarkers or combinations were evaluated using the receiver operating characteristic curve analysis. Signal intensities for gal‑1, gal‑3, CK19 and HBME‑1 were significantly greater in PC compared with FA (P<0.001). Conversely, expression levels of TPO were significantly increased in FA compared with PC (P<0.001). Gal‑3 and CK19 appeared to be the most sensitive markers (97 and 98%, respectively), whereas galectin‑1 was the most specific (97%). The combination of gal‑3, CK19 and HBME‑1 acted as the most efficient and informative marker panel reaching the greatest specificity (97%) and sensitivity (95%) for the diagnosis of PCs. The findings suggest that this combination of markers may improve the reliability of diagnosis of thyroid cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Arcolia V, Journe F, Renaud F, Leteurtre E, Gabius HJ, Remmelink M and Saussez S: Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer. Oncol Lett 14: 4183-4189, 2017.
APA
Arcolia, V., Journe, F., Renaud, F., Leteurtre, E., Gabius, H., Remmelink, M., & Saussez, S. (2017). Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer. Oncology Letters, 14, 4183-4189. https://doi.org/10.3892/ol.2017.6719
MLA
Arcolia, V., Journe, F., Renaud, F., Leteurtre, E., Gabius, H., Remmelink, M., Saussez, S."Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer". Oncology Letters 14.4 (2017): 4183-4189.
Chicago
Arcolia, V., Journe, F., Renaud, F., Leteurtre, E., Gabius, H., Remmelink, M., Saussez, S."Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer". Oncology Letters 14, no. 4 (2017): 4183-4189. https://doi.org/10.3892/ol.2017.6719
Copy and paste a formatted citation
x
Spandidos Publications style
Arcolia V, Journe F, Renaud F, Leteurtre E, Gabius HJ, Remmelink M and Saussez S: Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer. Oncol Lett 14: 4183-4189, 2017.
APA
Arcolia, V., Journe, F., Renaud, F., Leteurtre, E., Gabius, H., Remmelink, M., & Saussez, S. (2017). Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer. Oncology Letters, 14, 4183-4189. https://doi.org/10.3892/ol.2017.6719
MLA
Arcolia, V., Journe, F., Renaud, F., Leteurtre, E., Gabius, H., Remmelink, M., Saussez, S."Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer". Oncology Letters 14.4 (2017): 4183-4189.
Chicago
Arcolia, V., Journe, F., Renaud, F., Leteurtre, E., Gabius, H., Remmelink, M., Saussez, S."Combination of galectin-3, CK19 and HBME-1 immunostaining improves the diagnosis of thyroid cancer". Oncology Letters 14, no. 4 (2017): 4183-4189. https://doi.org/10.3892/ol.2017.6719
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