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Article Open Access

Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer

  • Authors:
    • Yan‑Shen Shan
    • Hui‑Ping Hsu
    • Ming‑Derg Lai
    • Yu‑Hsuan Hung
    • Chih‑Yang Wang
    • Meng‑Chi Yen
    • Yi‑Ling Chen
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, R.O.C., Department of Biochemistry and Molecular Biology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, R.O.C., Department of Emergency Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, R.O.C., Department of Senior Citizen Service Management, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan, R.O.C.
    Copyright: © Shan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4517-4526
    |
    Published online on: August 9, 2017
       https://doi.org/10.3892/ol.2017.6736
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Abstract

Overexpression of cyclin D is associated with the molecular tumorigenesis of gastric cancer. The purpose of the present study was to investigate the expression of cyclin D in human gastric cancer and to determine the potential correlations between cyclin D expression and clinicopathological characteristics of specific histological types, as well as its prognostic significance. In the present study, the expression of the cyclin D1 (CCND1), cyclin D2 (CCND2) and cyclin D3 (CCND3) genes in gastric cancer patients was explored using the Oncomine database, and their correlation with overall survival (OS) and progression‑free survival (PFS) was evalua­ted using Kaplan‑Meier analysis. The prognostic significance of CCND1 protein expression was evaluated by western blot analysis of 32 matched specimens of gastric adenocarcinomas and normal tissues obtained from patients treated at the National Cheng Kung University Hospital (Tainan, Taiwan). Analysis of the Oncomine cancer microarray database revealed that CCND1 gene expression was significantly increased in gastric intestinal‑type adenocarcinoma, while CCND2 was significantly increased in diffuse gastric adenocarcinoma, gastric intestinal‑type adenocarcinoma and gastric mixed adenocarcinoma. Kaplan‑Meier analysis indicated that overexpression of CCND1 was associated with reduced OS and PFS. In addition, overexpression of CCND1 and downregulation of CCND2 were significantly correla­ted with receptor tyrosine‑protein kinase erb‑2‑negative tumors and poor differe­ntiation. The ratio of relative CCND1 expression (expressed as the CCND1/β‑actin ratio) in tumor tissues compared with that in normal tissues was correlated with poor differentiation (P=0.0018). In summary, CCND1 overexpres­sion is associated with shorter survival in patients with gastric cancer and with poorly differentiated tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Shan YS, Hsu HP, Lai MD, Hung YH, Wang CY, Yen MC and Chen YL: Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer. Oncol Lett 14: 4517-4526, 2017.
APA
Shan, Y., Hsu, H., Lai, M., Hung, Y., Wang, C., Yen, M., & Chen, Y. (2017). Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer. Oncology Letters, 14, 4517-4526. https://doi.org/10.3892/ol.2017.6736
MLA
Shan, Y., Hsu, H., Lai, M., Hung, Y., Wang, C., Yen, M., Chen, Y."Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer". Oncology Letters 14.4 (2017): 4517-4526.
Chicago
Shan, Y., Hsu, H., Lai, M., Hung, Y., Wang, C., Yen, M., Chen, Y."Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer". Oncology Letters 14, no. 4 (2017): 4517-4526. https://doi.org/10.3892/ol.2017.6736
Copy and paste a formatted citation
x
Spandidos Publications style
Shan YS, Hsu HP, Lai MD, Hung YH, Wang CY, Yen MC and Chen YL: Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer. Oncol Lett 14: 4517-4526, 2017.
APA
Shan, Y., Hsu, H., Lai, M., Hung, Y., Wang, C., Yen, M., & Chen, Y. (2017). Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer. Oncology Letters, 14, 4517-4526. https://doi.org/10.3892/ol.2017.6736
MLA
Shan, Y., Hsu, H., Lai, M., Hung, Y., Wang, C., Yen, M., Chen, Y."Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer". Oncology Letters 14.4 (2017): 4517-4526.
Chicago
Shan, Y., Hsu, H., Lai, M., Hung, Y., Wang, C., Yen, M., Chen, Y."Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer". Oncology Letters 14, no. 4 (2017): 4517-4526. https://doi.org/10.3892/ol.2017.6736
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