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Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells

  • Authors:
    • Guanjie Zhao
    • Minfei Wu
    • Xiaofeng Wang
    • Zhenwu Du
    • Guizhen Zhang
  • View Affiliations / Copyright

    Affiliations: Research Center of The Second Hospital, Jilin University, Changchun, Jilin 130041, P.R. China, Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Department of Stomatology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4772-4778
    |
    Published online on: August 11, 2017
       https://doi.org/10.3892/ol.2017.6748
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Abstract

The aim of the present study was to examine the effect of fatty acid binding protein-5 (FABP-5) gene on the proliferation, apoptosis and invasion of human gastric cancer SGC-7901 cells. The viability, apoptosis and cell invasion of SGC-7901 cells before and after FABP5 knockdown were taken as the study objects, design and synthesis of siRNA interference sequence were conducted according to FABP-5 mRNA coding sequences, and SGC-7901 cells were transiently transfected. The human gastric cancer SGC-7901 cells were divided into three groups: FABP-5 siRNA group, negative control group and blank control group. FABP-5 gene mRNA and protein expression levels were detected by RT-PCR and western blot analysis. The CCK-8 assay was used to detect in vitro cell proliferation, flow cytometry (FCM) was used to detect changes in cell cycle and apoptosis in each group, TUNEL staining was used to detect apoptosis in each group, and the cell invasion chamber assay was used to detect cell invasiveness in each group. Each test was repeated three times. The results of the RT-PCR and western blot analysis showed that, expression of FABP-5 mRNA and protein in the FABP-5 siRNA group was significantly decreased compared with the negative and blank control groups. The cell growth rate in the FABP-5 siRNA group was significantly retarded, cell cycle was arrested in G0/G1 phase, the number of cells in S phase was reduced, and compared with the negative and blank control groups, the apoptotic rate in the FABP-5 siRNA group was significantly increased (P<0.01), while proliferation and invasiveness were significantly decreased (P<0.05). In conclusion, specific FABP-5 gene silencing may reduce the invasiveness of gastric cancer cells, inhibit cell proliferation, and arrest cell cycle in G0/G1 phase, resulting in a significant increase in apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao G, Wu M, Wang X, Du Z and Zhang G: Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells. Oncol Lett 14: 4772-4778, 2017.
APA
Zhao, G., Wu, M., Wang, X., Du, Z., & Zhang, G. (2017). Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells. Oncology Letters, 14, 4772-4778. https://doi.org/10.3892/ol.2017.6748
MLA
Zhao, G., Wu, M., Wang, X., Du, Z., Zhang, G."Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells". Oncology Letters 14.4 (2017): 4772-4778.
Chicago
Zhao, G., Wu, M., Wang, X., Du, Z., Zhang, G."Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells". Oncology Letters 14, no. 4 (2017): 4772-4778. https://doi.org/10.3892/ol.2017.6748
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao G, Wu M, Wang X, Du Z and Zhang G: Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells. Oncol Lett 14: 4772-4778, 2017.
APA
Zhao, G., Wu, M., Wang, X., Du, Z., & Zhang, G. (2017). Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells. Oncology Letters, 14, 4772-4778. https://doi.org/10.3892/ol.2017.6748
MLA
Zhao, G., Wu, M., Wang, X., Du, Z., Zhang, G."Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells". Oncology Letters 14.4 (2017): 4772-4778.
Chicago
Zhao, G., Wu, M., Wang, X., Du, Z., Zhang, G."Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells". Oncology Letters 14, no. 4 (2017): 4772-4778. https://doi.org/10.3892/ol.2017.6748
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