Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
October-2017 Volume 14 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2017 Volume 14 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Ovarian cancer proliferation and apoptosis are regulated by human transfer RNA methyltransferase 9-likevia LIN9

Corrigendum in: /10.3892/ol.2021.12459
  • Authors:
    • Huai Mei Chen
    • Jia Wang
    • Ying Feng Zhang
    • Yan Hong Gao
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, University‑Town Hospital of Chongqing Medical University, Chongqing 401331, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4461-4466
    |
    Published online on: August 14, 2017
       https://doi.org/10.3892/ol.2017.6750
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Current traditional treatment options have little impact on the long‑term survival of patients with ovarian cancer due to a lack of understanding of the molecular transformations that occur in ovarian carcinoma. Transfer RNAs (tRNAs) perform a key role in protein translational fidelity. Enzymes involved in tRNA modification may function as regulators of cancer progression. Human tRNA methyltransferase 9‑like (hTRM9L) catalyzes tRNA wobble base modifications, which regulate ovarian cancer growth and apoptosis via the retinoblastoma protein (pRB) and p53 signaling pathways. The aim of the present study was to confirm the role of hTRM9L in the proliferation and apoptosis of ovarian cancer. Immunohistochemistry was performed to investigate the expression of hTRM9L and LIN9 in 70 ovarian tissues. hTRM9L was amplified by polymerase chain reaction (PCR) and inserted into the Ubi‑multiple cloning site‑enhanced green fluorescent protein (EGFP)‑internal ribosome entry site‑puromycin lentiviral expression vector to create the Ubi-KIAA1456-EGFP‑puromycin (LV‑KIAA1456) vector. The lentiviruses were subsequently compounded and transduced into HO8910PM cells. hTRM9L, LIN9 and B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2 associated X protein (Bax) expression levels were examined by PCR and western blot analysis. Apoptosis was verified by flow cytometry, and cell proliferation was evaluated using Cell Counting Kit‑8. hTRM9L and LIN9 expression were reduced in the ovarian cancer group, and there was a positive correlation between hTRM9L and LIN9 expression according to Pearson's correlation coefficient (r=0.406; P<0.05). hTRM9L was increased by 2‑3‑foldin HO8910PM cells following LV‑hTRM9L transduction. The expression of hTRM9L at the mRNA and protein levels in HO8910PM cells that were transfected with LV‑hTRM9L was significantly increased compared with the negative control, as confirmed by reverse transcription‑quantitative PCR and western blot analysis, respectively (P<0.05).The same was observed for LIN9 and Bax (P<0.05). By contrast, Bcl‑2 was downregulated in LV‑hTRM9L (P<0.05). Furthermore, cell growth was inhibited (P<0.05) and apoptosis increased (P<0.05). In the present study, hTRM9L was shown to prevent tumor growth and promote apoptosis by regulating LIN9, which is associated with the pRB and p53 signaling pathways. This maybe a novel breakthrough in the treatment of ovarian cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Gay GM, Lim JS, Chay WY, Chow KY, Tan MH and Lim WY: Reproductive factors, adiposity, breastfeeding and their associations with ovarian cancer in an Asian cohort. Cancer Causes Control. 26:1561–1573. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Dinkelspiel HE, Champer M, Hou J, Tergas A, Burke WM, Huang Y, Neugut AI, Ananth CV, Hershman DL and Wright JD: Long-term mortality among women with epithelial ovarian cancer. Gynecol Oncol. 138:421–428. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Letoquart J, van Tran N, Caroline V, Aleksandrov A, Lazar N, van Tilbeurgh H, Liger D and Graille M: Insights into molecular plasticity in protein complexes from Trm9-Trm112 tRNA modifying enzyme crystal structure. Nucleic Acids Res. 43:10989–11002. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Begley U, Dyavaiah M, Patil A, Rooney JP, DiRenzo D, Young CM, Conklin DS, Zitomer RS and Begley TJ: Trm9-catalyzed tRNA modifications link translation to the DNA damage response. Mol Cell. 28:860–870. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Kalhor HR and Clarke S: Novel methyltransferase for modified uridine residues at the wobble position of tRNA. Mol Cell Biol. 23:9283–9292. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Wiseman EF, Chen X, Han N, Webber A, Ji Z, Sharrocks AD and Ang YS: Deregulation of the FOXM1 target gene network and its coregulatory partners in oesophageal adenocarcinoma. Mol Cancer. 14:692015. View Article : Google Scholar : PubMed/NCBI

8 

Flanagan JM, Healey S, Young J, Whitehall V, Trott DA, Newbold RF and Chenevix-Trench G: Mapping of a candidate colorectal cancer tumor-suppressor gene to a 900-kilobase region on the short arm of chromosome 8. Genes Chromosomes Cancer. 40:247–260. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Begley U, Sosa MS, Avivar-Valderas A, Patil A, Endres L, Estrada Y, Chan CT, Su D, Dedon PC, Aguirre-Ghiso JA and Begley T: A human tRNA methyltransferase 9-like protein prevents tumour growth by regulating LIN9 and HIF1-α. EMBO Mol Med. 5:366–383. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Bešević J, Gunter MJ, Fortner RT, Tsilidis KK, Weiderpass E, Onland-Moret Charlotte N, Dossus L, Tjønneland A, Hansen L, Overvad K, et al: Reproductive factors and epithelial ovarian cancer survival in the EPIC cohort study. Br J Cancer. 113:1622–1631. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Hauser S, Ulrich T, Wurster S, Schmitt K, Reichert N and Gaubatz S: Loss of LIN9, a member of the DREAM complex, cooperateswith SV40 large T antigen to induce genomic instability and anchorage-independent growth. Oncogene. 31:1859–1868. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Blais A, van Oevelen CJ, Margueron R, Acosta-Alvear D and Dynlacht BD: Retinoblastoma tumor suppressor protein-dependent methylation of histone H3 lysine 27 is associated with irreversible cell cycle exit. J Cell Biol. 179:1399–1412. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Hauser S, Ulrich T, Wurster S, Schmitt K, Reichert N and Gaubatz S: Loss of LIN9, a member of the DREAM complex, cooperates with SV40 large T antigen to induce genomic instability and anchorage-independent growth. Oncogene. 31:1859–1868. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Stowell KM: DNA testing for malignant hyperthermia: The reality and the dream. Anesth Analg. 118:397–406. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Tan KO, Nielsen AB, Meier BH and Ernst M: Broad-band DREAM recoupling sequence. J Phys Chem Lett. 5:3366–3372. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Yu Z, Wu F, Chen L, Li Q, Wang C, Dong J and Xie SQ: ETME, a novel β-elemene derivative, synergizes with arsenic trioxide in inducing apoptosis and cell cycle arrest in hepatocarcinoma cells via a p53-dependent pathway. Acta Pharm Sin B. 4:424–429. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Obata F, Tomioka K and Miura M: Transcriptional profiling of apoptosis-deficient Drosophila mutants. Genom Data. 2:254–257. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Carper MB, Denvir J, Boskovic G, Primerano DA and Claudio PP: RGS16, a novel p53 and pRb cross-talk candidate inhibits migration and invasion of pancreatic cancer cells. Genes Cancer. 5:420–435. 2014.PubMed/NCBI

19 

Zhou Z, Flesken-Nikitin A, Corney DC, Wang W, Goodrich DW, Roy-Burman P and Nikitin AY: Synergy of p53 and Rb deficiency in a conditional mouse model for metastatic prostate cancer. Cancer Res. 66:7889–7898. 2006. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen HM, Wang J, Zhang YF and Gao YH: Ovarian cancer proliferation and apoptosis are regulated by human transfer RNA methyltransferase 9-likevia LIN9 Corrigendum in /10.3892/ol.2021.12459. Oncol Lett 14: 4461-4466, 2017.
APA
Chen, H.M., Wang, J., Zhang, Y.F., & Gao, Y.H. (2017). Ovarian cancer proliferation and apoptosis are regulated by human transfer RNA methyltransferase 9-likevia LIN9 Corrigendum in /10.3892/ol.2021.12459. Oncology Letters, 14, 4461-4466. https://doi.org/10.3892/ol.2017.6750
MLA
Chen, H. M., Wang, J., Zhang, Y. F., Gao, Y. H."Ovarian cancer proliferation and apoptosis are regulated by human transfer RNA methyltransferase 9-likevia LIN9 Corrigendum in /10.3892/ol.2021.12459". Oncology Letters 14.4 (2017): 4461-4466.
Chicago
Chen, H. M., Wang, J., Zhang, Y. F., Gao, Y. H."Ovarian cancer proliferation and apoptosis are regulated by human transfer RNA methyltransferase 9-likevia LIN9 Corrigendum in /10.3892/ol.2021.12459". Oncology Letters 14, no. 4 (2017): 4461-4466. https://doi.org/10.3892/ol.2017.6750
Copy and paste a formatted citation
x
Spandidos Publications style
Chen HM, Wang J, Zhang YF and Gao YH: Ovarian cancer proliferation and apoptosis are regulated by human transfer RNA methyltransferase 9-likevia LIN9 Corrigendum in /10.3892/ol.2021.12459. Oncol Lett 14: 4461-4466, 2017.
APA
Chen, H.M., Wang, J., Zhang, Y.F., & Gao, Y.H. (2017). Ovarian cancer proliferation and apoptosis are regulated by human transfer RNA methyltransferase 9-likevia LIN9 Corrigendum in /10.3892/ol.2021.12459. Oncology Letters, 14, 4461-4466. https://doi.org/10.3892/ol.2017.6750
MLA
Chen, H. M., Wang, J., Zhang, Y. F., Gao, Y. H."Ovarian cancer proliferation and apoptosis are regulated by human transfer RNA methyltransferase 9-likevia LIN9 Corrigendum in /10.3892/ol.2021.12459". Oncology Letters 14.4 (2017): 4461-4466.
Chicago
Chen, H. M., Wang, J., Zhang, Y. F., Gao, Y. H."Ovarian cancer proliferation and apoptosis are regulated by human transfer RNA methyltransferase 9-likevia LIN9 Corrigendum in /10.3892/ol.2021.12459". Oncology Letters 14, no. 4 (2017): 4461-4466. https://doi.org/10.3892/ol.2017.6750
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team