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Article Open Access

Trigonostemon reidioides modulates endothelial cell proliferation, migration and tube formation via downregulation of the Akt signaling pathway

  • Authors:
    • Young‑Rak Cho
    • Kyuhee Park
    • Jae‑Shin Kang
    • Hye‑Woo Byun
    • Joa Sub Oh
    • Dong‑Wan Seo
    • Eun‑Kyung Ahn
  • View Affiliations / Copyright

    Affiliations: Bio‑Center, Gyeonggi Institute of Science and Technology Promotion, Suwon, Gyeonggi 16229, Republic of Korea, Biological Genetic Resources Utilization Division, National Institute of Biological Resources, Seo, Incheon 22689, Republic of Korea, Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungcheong 31116, Republic of Korea
    Copyright: © Cho et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4677-4683
    |
    Published online on: August 17, 2017
       https://doi.org/10.3892/ol.2017.6760
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Abstract

Trigonostemon reidi`oides (TR) is used as a Thai traditional medicine for the treatment of drug addiction, asthma, food poisoning, constipation and snake bites. The present study investigated the effects and molecular mechanisms of the ethanolic extract of TR (ETR) on mitogen‑induced human umbilical vein endothelial cells (HUVECs) responses, proliferation, adhesion, migration and tube formation. ETR treatment inhibited mitogen‑induced HUVEC proliferation by downregulation of cell cycle‑associated proteins, including cyclins and cyclin‑dependent kinases, which induced retinoblastoma protein hypophosphorylation. The present study also demonstrated that ETR treatment suppressed mitogen‑induced HUVEC adhesion, migration, invasion and tube formation, and that these anti‑angiogenic activities were mediated by inactivation of mitogen‑induced Akt and matrix metalloproteinase (MMP)‑2, but not of extracellular signal‑regulated kinase, p70 ribosomal S6 kinase or MMP‑9. Collectively, the results of the present study suggested pharmacological functions and molecular mechanisms of ETR in regulating endothelial cell fates, and supported further evaluation and development of ETR as a potential therapeutic agent for the treatment and prevention of angiogenesis‑associated diseases, including cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Cho YR, Park K, Kang JS, Byun HW, Oh JS, Seo DW and Ahn EK: Trigonostemon reidioides modulates endothelial cell proliferation, migration and tube formation via downregulation of the Akt signaling pathway. Oncol Lett 14: 4677-4683, 2017.
APA
Cho, Y., Park, K., Kang, J., Byun, H., Oh, J.S., Seo, D., & Ahn, E. (2017). Trigonostemon reidioides modulates endothelial cell proliferation, migration and tube formation via downregulation of the Akt signaling pathway. Oncology Letters, 14, 4677-4683. https://doi.org/10.3892/ol.2017.6760
MLA
Cho, Y., Park, K., Kang, J., Byun, H., Oh, J. S., Seo, D., Ahn, E."Trigonostemon reidioides modulates endothelial cell proliferation, migration and tube formation via downregulation of the Akt signaling pathway". Oncology Letters 14.4 (2017): 4677-4683.
Chicago
Cho, Y., Park, K., Kang, J., Byun, H., Oh, J. S., Seo, D., Ahn, E."Trigonostemon reidioides modulates endothelial cell proliferation, migration and tube formation via downregulation of the Akt signaling pathway". Oncology Letters 14, no. 4 (2017): 4677-4683. https://doi.org/10.3892/ol.2017.6760
Copy and paste a formatted citation
x
Spandidos Publications style
Cho YR, Park K, Kang JS, Byun HW, Oh JS, Seo DW and Ahn EK: Trigonostemon reidioides modulates endothelial cell proliferation, migration and tube formation via downregulation of the Akt signaling pathway. Oncol Lett 14: 4677-4683, 2017.
APA
Cho, Y., Park, K., Kang, J., Byun, H., Oh, J.S., Seo, D., & Ahn, E. (2017). Trigonostemon reidioides modulates endothelial cell proliferation, migration and tube formation via downregulation of the Akt signaling pathway. Oncology Letters, 14, 4677-4683. https://doi.org/10.3892/ol.2017.6760
MLA
Cho, Y., Park, K., Kang, J., Byun, H., Oh, J. S., Seo, D., Ahn, E."Trigonostemon reidioides modulates endothelial cell proliferation, migration and tube formation via downregulation of the Akt signaling pathway". Oncology Letters 14.4 (2017): 4677-4683.
Chicago
Cho, Y., Park, K., Kang, J., Byun, H., Oh, J. S., Seo, D., Ahn, E."Trigonostemon reidioides modulates endothelial cell proliferation, migration and tube formation via downregulation of the Akt signaling pathway". Oncology Letters 14, no. 4 (2017): 4677-4683. https://doi.org/10.3892/ol.2017.6760
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