Open Access

Notch1 suppresses prostate cancer cell invasion via the metastasis‑associated 1‑KiSS‑1 metastasis‑suppressor pathway

  • Authors:
    • Gang Deng
    • Xiaoliang Zheng
    • Peiwu Jiang
    • Kean Chen
    • Xiaoju Wang
    • Kang Jiang
    • Wenjun Zhang
    • Linglan Tu
    • Dongmei Yan
    • Libin Ma
    • Shenglin Ma
  • View Affiliations

  • Published online on: August 17, 2017     https://doi.org/10.3892/ol.2017.6761
  • Pages: 4477-4482
  • Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Notch1 is a type‑1 transmembrane receptor which has been demonstrated to be involved in proliferation in various organisms. A number of studies have proposed that Notch signaling may be aberrantly activated, thus contributing to development, invasion and metastasis in a variety of human cancers. In the present study, the function and mechanism of Notch1 in human prostate cancer (PCa) LNCaP cells in vitro was investigated. Notch1 and cleaved‑Notch1 expression were evaluated in human PCa cell lines, including LNCaP, PC‑3 and DU 145, and the human prostate epithelial RWPE‑1 cell line. LNCaP cells were transfected with Notch1‑targeting short hairpin RNAs (shRNAs) and the level of proliferation, the ability to invade and the expression of genes associated with cancer cell invasion were subsequently investigated. Notch1 was highly expressed in LNCaP, PC‑3 and DU 145 cells compared with RWPE‑1 cells, while cleaved‑Notch1 was expressed in LNCaP, PC‑3 and DU 145 cells, and only to a minimal extent in RWPE‑1 cells. Knockdown of Notch1 by shRNA in LNCaP cells markedly decreased cell invasion through Matrigel and inhibited cell proliferation 48 h following transfection. Reverse transcription‑quantitative polymerase chain reaction analysis indicated that Notch1‑knockdown resulted in a significant reduction of metastasis‑associated 1 (MTA1) and increase of KiSS‑1 metastasis‑suppressor (KISS‑1), mitogen‑activated protein kinase 4 (MKK4) and cluster of differentiation 82 (KAI1). The present data demonstrated that expression of Notch1 was significantly associated with the invasion of prostate cancer. Knockdown of Notch1 decreased the invasive ability of LNCaP cells, which may be caused by downregulating MTA1 and upregulating KISS‑1, MKK4 and KAI1. These findings indicated that targeting Notch1 may provide a novel method of suppressing or treating metastasis in prostate cancer.
View Figures
View References

Related Articles

Journal Cover

October-2017
Volume 14 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Deng G, Zheng X, Jiang P, Chen K, Wang X, Jiang K, Zhang W, Tu L, Yan D, Ma L, Ma L, et al: Notch1 suppresses prostate cancer cell invasion via the metastasis‑associated 1‑KiSS‑1 metastasis‑suppressor pathway. Oncol Lett 14: 4477-4482, 2017
APA
Deng, G., Zheng, X., Jiang, P., Chen, K., Wang, X., Jiang, K. ... Ma, S. (2017). Notch1 suppresses prostate cancer cell invasion via the metastasis‑associated 1‑KiSS‑1 metastasis‑suppressor pathway. Oncology Letters, 14, 4477-4482. https://doi.org/10.3892/ol.2017.6761
MLA
Deng, G., Zheng, X., Jiang, P., Chen, K., Wang, X., Jiang, K., Zhang, W., Tu, L., Yan, D., Ma, L., Ma, S."Notch1 suppresses prostate cancer cell invasion via the metastasis‑associated 1‑KiSS‑1 metastasis‑suppressor pathway". Oncology Letters 14.4 (2017): 4477-4482.
Chicago
Deng, G., Zheng, X., Jiang, P., Chen, K., Wang, X., Jiang, K., Zhang, W., Tu, L., Yan, D., Ma, L., Ma, S."Notch1 suppresses prostate cancer cell invasion via the metastasis‑associated 1‑KiSS‑1 metastasis‑suppressor pathway". Oncology Letters 14, no. 4 (2017): 4477-4482. https://doi.org/10.3892/ol.2017.6761