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Article

Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post‑bupivacaine

  • Authors:
    • Gong‑Sheng Li
    • Gao‑Yin Kong
    • Yi Zou
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Hunan Provincial People's Hospital, Changsha, Hunan 410002, P.R. China
  • Pages: 5013-5017
    |
    Published online on: August 18, 2017
       https://doi.org/10.3892/ol.2017.6773
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Abstract

The present study aimed to investigate the potential effect of leucine‑rich repeat containing 3B (LRRC3B) with respect to the inhibition of breast cancer recurrence and metastasis post‑anesthesia. The mRNA expression of LRRC3B in breast MDA‑MB‑231 and MCF‑7 cell lines was detected using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis. The effect of bupivacaine on breast cancer cell invasion was analyzed using a Matrigel assay. LRRC3B specific small interfering (si)RNA was constructed and transfected into breast cancer cells using Lipofectamine® 2000 reagent. The influence of bupivacaine on LRRC3B expression was measured based on RT‑qPCR. Additionally, the effect of LRRC3B silencing on the invasion of breast cancer cells treated with bupivacaine was analyzed. Compared with the control, LRRC3B expression significantly increased in MDA‑MB‑231 and in MCF‑7 cells as the length of time increased (P<0.05), but the expression of the gene significantly declined in 2 types of cancer cell when the cells were transfected with siRNA‑LRRC3B plasma (P<0.05). The administration of 50 µg/ml bupivacaine promoted maximum breast cancer cell invasion, and suppressed LRRC3B mRNA expression in cells. However, when LRRC3B was silenced in cancer cells, 20 µg/ml bupivacaine significantly promoted cancer cell invasion, indicating that bupivacaine suppresses the expression of LRRC3B and promotes cell invasion. The present study suggested that LRRC3B serves a protective role in preventing bupivacaine‑induced breast cancer recurrence and metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Li GS, Kong GY and Zou Y: Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post‑bupivacaine. Oncol Lett 14: 5013-5017, 2017.
APA
Li, G., Kong, G., & Zou, Y. (2017). Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post‑bupivacaine. Oncology Letters, 14, 5013-5017. https://doi.org/10.3892/ol.2017.6773
MLA
Li, G., Kong, G., Zou, Y."Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post‑bupivacaine". Oncology Letters 14.4 (2017): 5013-5017.
Chicago
Li, G., Kong, G., Zou, Y."Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post‑bupivacaine". Oncology Letters 14, no. 4 (2017): 5013-5017. https://doi.org/10.3892/ol.2017.6773
Copy and paste a formatted citation
x
Spandidos Publications style
Li GS, Kong GY and Zou Y: Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post‑bupivacaine. Oncol Lett 14: 5013-5017, 2017.
APA
Li, G., Kong, G., & Zou, Y. (2017). Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post‑bupivacaine. Oncology Letters, 14, 5013-5017. https://doi.org/10.3892/ol.2017.6773
MLA
Li, G., Kong, G., Zou, Y."Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post‑bupivacaine". Oncology Letters 14.4 (2017): 5013-5017.
Chicago
Li, G., Kong, G., Zou, Y."Protective role of LRRC3B in preventing breast cancer metastasis and recurrence post‑bupivacaine". Oncology Letters 14, no. 4 (2017): 5013-5017. https://doi.org/10.3892/ol.2017.6773
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