Open Access

Autophagy mediates free fatty acid effects on MDA‑MB‑231 cell proliferation, migration and invasion

  • Authors:
    • Juan Wu
    • Qi Wu
    • Juan‑Juan Li
    • Chuang Chen
    • Si Sun
    • Chang‑Hua Wang
    • Sheng‑Rong Sun
  • View Affiliations

  • Published online on: August 24, 2017     https://doi.org/10.3892/ol.2017.6807
  • Pages: 4715-4721
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Epidemiological and animal studies indicate an association between high levels of dietary fat intake and an increased risk of breast cancer. The multifaceted role of autophagy in cancer has been revealed in previous years. However, the mechanism of this role remains unknown. In the present study, the two most common free fatty acids, palmitate acid (PA) and oleic acid (OA), were used to deter­mine the effect on human breast cancer MDA‑MB‑231 cells, and the possible role of autophagy was investigated by detecting light chain 3 (LC3)‑II/I. Bafliomycin A1 was used to detect autophagy flux. High palmitate acid condition‑induced MDA‑MB‑231 cell death and invasion were mitigated by 3‑methyladenine pretreatment or transfection with shRNA against autophagy protein 5. By contrast, high oleic acid condition induced MDA‑MB‑231 cell proliferation, migration and invasion were mitigated using rapamycin. The present results suggest that autophagy has an important role in the effects of PA and OA on breast cancer growth and metastasis in vitro.
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October-2017
Volume 14 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Wu J, Wu Q, Li JJ, Chen C, Sun S, Wang CH and Sun SR: Autophagy mediates free fatty acid effects on MDA‑MB‑231 cell proliferation, migration and invasion. Oncol Lett 14: 4715-4721, 2017
APA
Wu, J., Wu, Q., Li, J., Chen, C., Sun, S., Wang, C., & Sun, S. (2017). Autophagy mediates free fatty acid effects on MDA‑MB‑231 cell proliferation, migration and invasion. Oncology Letters, 14, 4715-4721. https://doi.org/10.3892/ol.2017.6807
MLA
Wu, J., Wu, Q., Li, J., Chen, C., Sun, S., Wang, C., Sun, S."Autophagy mediates free fatty acid effects on MDA‑MB‑231 cell proliferation, migration and invasion". Oncology Letters 14.4 (2017): 4715-4721.
Chicago
Wu, J., Wu, Q., Li, J., Chen, C., Sun, S., Wang, C., Sun, S."Autophagy mediates free fatty acid effects on MDA‑MB‑231 cell proliferation, migration and invasion". Oncology Letters 14, no. 4 (2017): 4715-4721. https://doi.org/10.3892/ol.2017.6807