AGTR1 promoter hypermethylation in lung squamous cell carcinoma but not in lung adenocarcinoma

Chen,Ruhua; Hong,Qingxiao; Jiang,Jianzhong; Chen,Xiaoying; Jiang,Zhenhuan; Wang,Jinzhi; Liu,Shunlin; Duan,Shiwei; Shi,Shunbin

doi:10.3892/ol.2017.6824

AGTR1 promoter hypermethylation in lung squamous cell carcinoma but not in lung adenocarcinoma

Authors:
- Ruhua Chen
- Qingxiao Hong
- Jianzhong Jiang
- Xiaoying Chen
- Zhenhuan Jiang
- Jinzhi Wang
- Shunlin Liu
- Shiwei Duan
- Shunbin Shi
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Affiliations: Department of Respiratory Medicine, Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China, Laboratory of Behavioral Neuroscience, Ningbo Institute of Microcirculation and Henbane, Ningbo Addiction Research and Treatment Center, Ningbo, Zhejiang 315010, P.R. China, Department of Geriatrics, Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China, Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Department of Orthopaedics, Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China, Department of Cell Biology, School of Medicine, Soochow University, Suzhou, Jiangsu 215000, P.R. China, Department of Respiratory Medicine, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Thoracic Surgery, Affiliated Wujiang Hospital of Nantong University, Wujiang, Suzhou, Jiangsu 215000, P.R. China
Published online on: August 25, 2017 https://doi.org/10.3892/ol.2017.6824
Pages: 4989-4994

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Abstract

Aberrant DNA methylation is associated with non‑small cell lung cancer (NSCLC), suggesting that gene promoter methylation may be a potential biomarker for the detection or risk prediction of NSCLC. The present study aimed to evaluate the potential usage of angiotensin II receptor type 1 (AGTR1) methylation in two major pathologic subtypes: Lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Quantitative methylation‑specific polymerase chain reaction was used to investigate the effect of AGTR1 promoter methylation in the tumor and the paired adjacent non‑tumor tissue samples from 42 patients with LUSC, and 69 with LUAD. The percentage of methylated reference was calculated and presented as the median (interquartile range 25th‑75th percentile). The results of the current study revealed that there was significantly increased AGTR1 promoter methylation in the tumor tissues compared with the paired adjacent non‑tumor tissue [97.4 (57.22‑130.5) vs. 85 (48.25‑123); P=0.024]. Furthermore, higher AGTR1 promoter methylation was observed in patients with LUSC compared with LUAD (odds ratio=2.483; 95% confidence interval=1.125‑5.480; P=0.023). Significant differences were identified in AGTR1 methylation between non‑tumor and the tumor tissues in LUSC [113.5 (68.33‑148.73) vs. 93.04 (45.94‑140); P=0.008]. In addition, the Cancer Genome Atlas data of 378 patients with LUSC and 477 with LUAD revealed an inverse correlation between gene expression and the methylation status of AGTR1 promoter.. These data suggest that AGTR1 hypermethylation is a promising biomarker to assist in LUSC detection and diagnosis.

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