Stability analysis on the radioactive iodine‑labelled prostate cancer‑specific recombinant oncolytic adenovirus

Zhou,Jiahe; Hao,Lin; Shi,Zhenduo; Ning,Songyi; He,Houguang; Zhao,Yan; Dong,Yang; Li,Zhigang; He,Jiuxiang; Zang,Guanghui; Han,Conghui

doi:10.3892/ol.2017.6998

December-2017 Volume 14 Issue 6

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December-2017 Volume 14 Issue 6

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Article Open Access

Stability analysis on the radioactive iodine‑labelled prostate cancer‑specific recombinant oncolytic adenovirus

Authors:
- Jiahe Zhou
- Lin Hao
- Zhenduo Shi
- Songyi Ning
- Houguang He
- Yan Zhao
- Yang Dong
- Zhigang Li
- Jiuxiang He
- Guanghui Zang
- Conghui Han
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Affiliations: Department of Urinary Surgery, Xuzhou School of Clinical Medicine Affiliated to Xuzhou Medicine University, Xuzhou, Jiangsu 221009, P.R. China, Jiangsu University School of Medicine, Zhenjiang, Jiangsu 212013, P.R. China, Kunshan Ruike Research and Development of Medicine Co., Ltd., Kunshan, Jiangsu 215300, P.R. China, Chongqing Western Biomedical Technology Co., Chongqing 400010, P.R. China

Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 6403-6408
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Published online on: September 19, 2017

https://doi.org/10.3892/ol.2017.6998
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Abstract

The aim of the present study was to construct the 125I‑replication‑selective oncolytic adenovirus (RSOAds)‑ human telomerase reverse transcriptase (hTERT)/prostate specific antigen (PSA) nuclide‑oncolytic virus marker by labelling the hTERT/PSA double‑regulation replicative oncolytic adenovirus with 125I nuclide, and investigate the influence of viral markers under various reaction conditions on labelling efficiency. N‑bromosuccinimide (NBS) was used as the oxidizer for 125I labelling, and the best conditions for labelling were identified through the reactions between oncolytic adenovirus at various concentrations and NBS. Dosage of 125I, reaction duration, pH values and reaction volume were respectively evaluated to determine their effects on the labelling efficiency of 125I‑RSOAds‑hTERT/PSA nuclide‑oncolytic adenovirus markers. Purified nuclide‑oncolytic adenovirus markers were isolated by gel‑filtration chromatography; paper chromatography was performed to assay the radiochemical purity of 125I‑RSOAds‑hTERT/PSA markers at various time points. Radiochemical purity of 125I‑RSOAds‑hTERT/PSA was >95%, and could be maintained at 4˚C for 7 days. The best reaction conditions were set as follows: 0.5 µl of 125I (~0.2 m Ci, 7.4 MBq); 25 qg of NBS; 100 µl of 8x109 VP/ml 125I‑RSOAds‑hTERT/PSA virus solution; 30 min of reaction duration; pH 7.5; 120 µl of PBS. Labelling hTERT/PSA double‑regulation replicative oncolytic adenovirus with 125I was identified to be available, and the radiochemical purity of acquired virus markers could be maintained under specific conditions.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Stability analysis on the radioactive iodine‑labelled prostate cancer‑specific recombinant oncolytic adenovirus

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Abstract

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