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Stomatin‑like protein 2 is overexpressed in cervical cancer and involved in tumor cell apoptosis

  • Authors:
    • Huan Deng
    • Yongjian Deng
    • Feiye Liu
    • Jie Chen
    • Zheng Li
    • Kelei Zhao
    • Xiaoqian Guan
    • Weijiang Liang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Cancer Center, Traditional Chinese Medicine‑Integrated Hospital, Southern Medical University, Guangzhou, Guangdong 510315, P.R. China, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Obstetrics and Gynecology, Cancer Center, Sun Yat‑sen University, Guangzhou, Guangdong 510060, P.R. China, Department of Oncology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6355-6364
    |
    Published online on: September 25, 2017
       https://doi.org/10.3892/ol.2017.7054
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Abstract

Stomatin‑like protein 2 (SLP‑2) is overexpressed in numerous types of human cancer and previous studies revealed that SLP‑2 may function in mitochondria. The purpose of the present study was to evaluate the expression of SLP‑2 in cervical cancer and the association between SLP‑2 expression and clinical features, in addition to investigating the role of SLP‑2 in the apoptosis of cervical cancer cells. The expression profile of SLP‑2 was determined by quantitative polymerase chain reaction, western blotting and immunohistochemical staining. The effect of SLP‑2 on cell apoptosis induced by chemotherapeutics in cervical cancer cells was evaluated using Annexin V staining and terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling (TUNEL) assays. The results indicated that SLP‑2 expression in cervical cancer was significantly upregulated at the mRNA and protein levels, compared with that in normal cervical tissues. Immunohistochemical analysis revealed significant correlation between SLP‑2 protein expression and clinical characteristics, including the squamous cell carcinoma antigen (P=0.003), deep stromal invasion (P=0.021), lymphovascular space involvement (P=0.044) and pelvic lymph node metastasis (P<0.001), which served as independent prognostic factors for predicting the shortening of overall survival time in patients with early‑stage cervical cancer. In addition, TUNEL and Annexin V binding assays revealed that silencing SLP‑2 expression significantly enhanced the sensitivity of cervical cancer cells to apoptosis induced by chemotherapeutics. Taken together, the results of the present study suggest that SLP‑2 may be a progressive gene in the development of cervical cancer. Overexpression of SLP‑2 serves an important role in the apoptosis of human cervical cancer cells.
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1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Barnholtz-Sloan J, Patel N, Rollison D, Kortepeter K, MacKinnon J and Giuliano A: Incidence trends of invasive cervical cancer in the United States by combined race and ethnicity. Cancer Causes Control. 20:1129–1138. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Benedet JL, Bender H, Jones H III, Ngan HY and Pecorelli S: FIGO staging classifications and clinical practice guidelines in the management of gynecologic cancers. FIGO Committee on Gynecologic Oncology. Int J Gynaecol Obstet. 70:209–262. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Sherman ME, Wang SS, Carreon J and Devesa SS: Mortality trends for cervical squamous and adenocarcinoma in the United States. Relation to incidence and survival. Cancer. 103:1258–1264. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Stewart GW: Stomatin. Int J Biochem Cell Biol. 29:271–274. 1997. View Article : Google Scholar : PubMed/NCBI

6 

Seidel G and Prohaska R: Molecular cloning of hSLP-1, a novel human brain-specific member of the band 7/MEC-2 family similar to Caenorhabditis elegans UNC-24. Gene. 225:23–29. 1998. View Article : Google Scholar : PubMed/NCBI

7 

Goldstein BJ, Kulaga HM and Reed RR: Cloning and characterization of SLP3: A novel member of the stomatin family expressed by olfactory receptor neurons. J Assoc Res Otolaryngol. 4:74–82. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Wang Y and Morrow JS: Identification and characterization of human SLP-2, a novel homologue of stomatin (band 7.2b) present in erythrocytes and other tissues. J Biol Chem. 275:8062–8071. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Hajek P, Chomyn A and Attardi G: Identification of a novel mitochondrial complex containing mitofusin 2 and stomatin-like protein 2. J Biol Chem. 282:5670–5681. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Zhang LY, Ding F, Liu ZM, Li WD, Liu ZH and Li YD: Effect of stomatin-like protein 2 (SLP-2) gene on growth and proliferation of esophageal squamous carcinoma cell line TE12. Ai Zheng. 24:155–159. 2005.(In Chinese). PubMed/NCBI

11 

Cao WF, Zhang LY, Liu MB, Tang PZ, Liu ZH and Sun BC: Prognostic significance of stomatin-like protein 2 overexpression in laryngeal squamous cell carcinoma: Clinical, histologic and immunohistochemistry analyses with tissue microarray. Hum Pathol. 38:747–752. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Zhang L, Ding F, Cao W and Liu Z, Liu W, Yu Z, Wu Y, Li W, Li Y and Liu Z: Stomatin-like protein 2 is overexpressed in cancer and involved in regulating cell growth and cell adhesion in human esophageal squamous cell carcinoma. Clin Cancer Res. 12:1639–1646. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Chevallet M, Lescuyer P, Diemer H, van Dorsselaer A, Leize-Wagner E and Rabilloud T: Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view. Electrophoresis. 27:1574–1583. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Marchiole P, Buénerd A, Benchaib M, Nezhat K, Dargent D and Mathevet P: Clinical significance of lympho vascular space involvement and lymph node micrometastases in early-stage cervical cancer: A retrospective case-control surgico-pathological study. Gynecol Oncol. 97:727–732. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Chernofsky MR, Felix JC, Muderspach LI, Morrow CP, Ye W, Groshen SG and Roman LD: Influence of quantity of lymph vascular space invasion on time to recurrence in women with early-stage squamous cancer of the cervix. Gynecol Oncol. 100:288–293. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Monk BJ, Wang J, Im S, Stock RJ, Peters WA III, Liu PY, Barrett RJ II, Berek JS, Souhami L, Grigsby PW, et al: Rethinking the use of radiation and chemotherapy after radical hysterectomy: A clinical-pathologic analysis of a gynecologic oncology group/southwest oncology group/radiation therapy oncology group trial. Gynecol Oncol. 96:721–728. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Keys HM, Bundy BN, Stehman FB, Muderspach LI, Chafe WE, Suggs CL III, Walker JL and Gersell D: Cisplatin, radiation, and adjuvant hysterectomy compared with radiation and adjuvant hysterectomy for bulky stage IB cervical carcinoma. N Engl J Med. 340:1154–1161. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Morris M, Eifel PJ, Lu J, Grigsby PW, Levenback C, Stevens RE, Rotman M, Gershenson DM and Mutch DG: Pelvic radiation with concurrent chemotherapy compared with pelvic and para-aortic radiation for high-risk cervical cancer. N Engl J Med. 340:1137–1143. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Selman TJ, Mann C, Zamora J, Appleyard TL and Khan K: Diagnostic accuracy of tests for lymph node status in primary cervical cancer: A systematic review and meta-analysis. CMAJ. 178:855–862. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Anzai Y, Piccoli CW, Outwater EK, Stanford W, Bluemke DA, Nurenberg P, Saini S, Maravilla KR, Feldman DE, Schmiedl UP, et al: Evaluation of neck and body metastases to nodes with ferumoxtran 10-enhanced MR imaging: Phase III safety and efficacy study. Radiology. 228:777–788. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Kim JH, Beets GL, Kim MJ, Kessels AG and Beets-Tan RG: High-resolution MR imaging for nodal staging in rectal cancer: Are there any criteria in addition to the size? Eur J Radiol. 52:78–83. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Perigaud C, Bridji B, Roussel JC, Sagan C, Mugniot A, Duveau D, Baron O and Despins P: Prospective preoperative mediastinal lymph node staging by integrated positron emission tomography-computerised tomography in patients with non-small-cell lung cancer. Eur J Cardiothorac Surg. 36:731–736. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Da Cruz S, Parone PA, Gonzalo P, Bienvenut WV, Tondera D, Jourdain A, Quadroni M and Martinou JC: SLP-2 interacts with prohibitins in the mitochondrial inner membrane and contributes to their stability. Biochim Biophys Acta. 1783:904–911. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Da Cruz S, De Marchi U, Frieden M, Parone PA, Martinou JC and Demaurex N: SLP-2 negatively modulates mitochondrial sodium-calcium exchange. Cell Calcium. 47:11–18. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Tondera D, Grandemange S, Jourdain A, Karbowski M, Mattenberger Y, Herzig S, Da Cruz S, Clerc P, Raschke I, Merkwirth C, et al: SLP-2 is required for stress-induced mitochondrial hyperfusion. EMBO J. 28:1589–1600. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Wang Y, Cao W, Yu Z and Liu Z: Downregulation of a mitochondria associated protein SLP-2 inhibits tumor cell motility, proliferation and enhances cell sensitivity to chemotherapeutic reagents. Cancer Biol Ther. 8:1651–1658. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Adrain C and Martin SJ: The mitochondrial apoptosome: A killer unleashed by the cytochrome seas. Trends Biochem Sci. 26:390–397. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Velde C Vande, Cizeau J, Dubik D, Alimonti J, Brown T, Israels S, Hakem R and Greenberg AH: BNIP3 and genetic control of necrosis-like cell death through the mitochondrial permeability transition pore. Mol Cell Biol. 20:5454–5468. 2000. View Article : Google Scholar : PubMed/NCBI

30 

Berridge MJ, Lipp P and Bootman MD: The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol. 1:11–21. 2000. View Article : Google Scholar : PubMed/NCBI

31 

McConkey DJ and Nutt LK: Calcium flux measurements in apoptosis. Methods Cell Biol. 66:229–246. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Deng H, Deng Y, Liu F, Chen J, Li Z, Zhao K, Guan X and Liang W: Stomatin‑like protein 2 is overexpressed in cervical cancer and involved in tumor cell apoptosis. Oncol Lett 14: 6355-6364, 2017.
APA
Deng, H., Deng, Y., Liu, F., Chen, J., Li, Z., Zhao, K. ... Liang, W. (2017). Stomatin‑like protein 2 is overexpressed in cervical cancer and involved in tumor cell apoptosis. Oncology Letters, 14, 6355-6364. https://doi.org/10.3892/ol.2017.7054
MLA
Deng, H., Deng, Y., Liu, F., Chen, J., Li, Z., Zhao, K., Guan, X., Liang, W."Stomatin‑like protein 2 is overexpressed in cervical cancer and involved in tumor cell apoptosis". Oncology Letters 14.6 (2017): 6355-6364.
Chicago
Deng, H., Deng, Y., Liu, F., Chen, J., Li, Z., Zhao, K., Guan, X., Liang, W."Stomatin‑like protein 2 is overexpressed in cervical cancer and involved in tumor cell apoptosis". Oncology Letters 14, no. 6 (2017): 6355-6364. https://doi.org/10.3892/ol.2017.7054
Copy and paste a formatted citation
x
Spandidos Publications style
Deng H, Deng Y, Liu F, Chen J, Li Z, Zhao K, Guan X and Liang W: Stomatin‑like protein 2 is overexpressed in cervical cancer and involved in tumor cell apoptosis. Oncol Lett 14: 6355-6364, 2017.
APA
Deng, H., Deng, Y., Liu, F., Chen, J., Li, Z., Zhao, K. ... Liang, W. (2017). Stomatin‑like protein 2 is overexpressed in cervical cancer and involved in tumor cell apoptosis. Oncology Letters, 14, 6355-6364. https://doi.org/10.3892/ol.2017.7054
MLA
Deng, H., Deng, Y., Liu, F., Chen, J., Li, Z., Zhao, K., Guan, X., Liang, W."Stomatin‑like protein 2 is overexpressed in cervical cancer and involved in tumor cell apoptosis". Oncology Letters 14.6 (2017): 6355-6364.
Chicago
Deng, H., Deng, Y., Liu, F., Chen, J., Li, Z., Zhao, K., Guan, X., Liang, W."Stomatin‑like protein 2 is overexpressed in cervical cancer and involved in tumor cell apoptosis". Oncology Letters 14, no. 6 (2017): 6355-6364. https://doi.org/10.3892/ol.2017.7054
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