Ouabain targets the Na+/K+‑ATPase α3 isoform to inhibit cancer cell proliferation and induce apoptosis

Xiao,Yijun; Meng,Chen; Lin,Jie; Huang,Chaoqun; Zhang,Xiuli; Long,Yanyu; Huang,Yide; Lin,Yao

doi:10.3892/ol.2017.7070

December-2017 Volume 14 Issue 6

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December-2017 Volume 14 Issue 6

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Article Open Access

Ouabain targets the Na+/K+‑ATPase α3 isoform to inhibit cancer cell proliferation and induce apoptosis

Authors:
- Yijun Xiao
- Chen Meng
- Jie Lin
- Chaoqun Huang
- Xiuli Zhang
- Yanyu Long
- Yide Huang
- Yao Lin
View Affiliations / Copyright

Affiliations: College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, P.R. China

Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 6678-6684
|
Published online on: September 26, 2017

https://doi.org/10.3892/ol.2017.7070
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Abstract

Ouabain has been used for the treatment of heart failure and atrial fibrillation. Its potential anticancer effect has also attracted great interest. The aim of the present study was to evaluate the anticancer effect of ouabain and investigate its molecular target. The effects of ouabain on the viability of and induction of cellular death on OS‑RC‑2 renal cancer cells were examined using the MTT assay and acridine orange/ethidium bromide staining. The levels of Ca2+ and reactive oxygen species were determined using Fura‑3‑acetoxymethyl ester and dichloro‑dihydro‑fluorescein diacetate probes, respectively. Apoptosis was examined using annexin V‑fluorescein isothiocyanate/propidium iodide staining and western blotting. The expression profile of the different Na+/K+‑ATPase (NKA) isoforms in NCI‑H446 small cell lung cancer cells was determined using immunocytochemistry and reverse transcription polymerase chain reaction analysis. In the present study, it was demonstrated that ouabain inhibited cancer cell proliferation and induced apoptosis while no significant difference in the expression of NKA α1 and α3 isoforms was detected following 48 h of ouabain treatment. Furthermore, expression of NKA α3 but not the α1 isoform was associated with ouabain sensitivity. The results of the present study indicated that ouabain targets the NKA α3 isoform, inhibits cancer cell proliferation and induces apoptosis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Ouabain targets the Na+/K+‑ATPase α3 isoform to inhibit cancer cell proliferation and induce apoptosis

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