Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway

Wang,Tongxin; Chen,Zhixia; Zhang,Wei

doi:10.3892/ol.2017.7103

Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway

Authors:
- Tongxin Wang
- Zhixia Chen
- Wei Zhang
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Affiliations: Department of Neurosurgery, Yidu Central Hospital of Weifang, Qingzhou, Shandong 262500, P.R. China
Published online on: September 29, 2017 https://doi.org/10.3892/ol.2017.7103
Pages: 7197-7200
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The objective of this study was to investigate the mechanism of the function of Wnt signaling pathway in regulating autophagy and inflammatory response in glioma cells. Human brain glioma cells U118 were selected and divided into three groups: i) the Wnt signaling inhibitor IWR-1 group (the observation group); ii) the PBS negative control group (the PBS group) and iii) the blank control group. After 24 h culture, Wnt5a/β-catenin protein, autophagy marker, microtubule-associated-proteins-1A1B-light-chain-3C (LC-3) II and Beclin I, and inflammatory factors IL-6 and TNF-α protein expression levels were evaluated using western blotting. Compared with both control groups, Wnt5a/β-catenin, IL-6 and TNF-α protein expression levels were significantly lower, and LC-3II and Beclin I protein expression levels were significantly higher in the observation group. In conclusion, Wnt5a/β-catenin signaling pathway regulates autophagy and inflammatory response of glioma cells.

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