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Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway

  • Authors:
    • Tongxin Wang
    • Zhixia Chen
    • Wei Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Yidu Central Hospital of Weifang, Qingzhou, Shandong 262500, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7197-7200
    |
    Published online on: September 29, 2017
       https://doi.org/10.3892/ol.2017.7103
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Abstract

The objective of this study was to investigate the mechanism of the function of Wnt signaling pathway in regulating autophagy and inflammatory response in glioma cells. Human brain glioma cells U118 were selected and divided into three groups: i) the Wnt signaling inhibitor IWR-1 group (the observation group); ii) the PBS negative control group (the PBS group) and iii) the blank control group. After 24 h culture, Wnt5a/β-catenin protein, autophagy marker, microtubule-associated-proteins-1A1B-light-chain-3C (LC-3) II and Beclin I, and inflammatory factors IL-6 and TNF-α protein expression levels were evaluated using western blotting. Compared with both control groups, Wnt5a/β-catenin, IL-6 and TNF-α protein expression levels were significantly lower, and LC-3II and Beclin I protein expression levels were significantly higher in the observation group. In conclusion, Wnt5a/β-catenin signaling pathway regulates autophagy and inflammatory response of glioma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Wang T, Chen Z and Zhang W: Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway. Oncol Lett 14: 7197-7200, 2017.
APA
Wang, T., Chen, Z., & Zhang, W. (2017). Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway. Oncology Letters, 14, 7197-7200. https://doi.org/10.3892/ol.2017.7103
MLA
Wang, T., Chen, Z., Zhang, W."Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway". Oncology Letters 14.6 (2017): 7197-7200.
Chicago
Wang, T., Chen, Z., Zhang, W."Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway". Oncology Letters 14, no. 6 (2017): 7197-7200. https://doi.org/10.3892/ol.2017.7103
Copy and paste a formatted citation
x
Spandidos Publications style
Wang T, Chen Z and Zhang W: Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway. Oncol Lett 14: 7197-7200, 2017.
APA
Wang, T., Chen, Z., & Zhang, W. (2017). Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway. Oncology Letters, 14, 7197-7200. https://doi.org/10.3892/ol.2017.7103
MLA
Wang, T., Chen, Z., Zhang, W."Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway". Oncology Letters 14.6 (2017): 7197-7200.
Chicago
Wang, T., Chen, Z., Zhang, W."Regulation of autophagy inhibition and inflammatory response in glioma by Wnt signaling pathway". Oncology Letters 14, no. 6 (2017): 7197-7200. https://doi.org/10.3892/ol.2017.7103
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