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Article

Combination of cecropinXJ and LY294002 induces synergistic cytotoxicity, and apoptosis in human gastric cancer cells via inhibition of the PI3K/Akt signaling pathway

  • Authors:
    • Li‑Jie Xia
    • Yan‑Ling Wu
    • Fu‑Chun Zhang
  • View Affiliations / Copyright

    Affiliations: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang 830046, P.R. China
  • Pages: 7522-7528
    |
    Published online on: October 2, 2017
       https://doi.org/10.3892/ol.2017.7112
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Abstract

The aim of the present study was to investigate the cytotoxic and apoptotic effects of cecropinXJ against human gastric cancer BGC823 cells, either alone, or in combination with a specific phosphatidylinositol 3‑kinase (PI3K) inhibitor, LY294002. Cell viability and the apoptosis rate were measured using flow cytometry with Annexin‑V staining. Additionally, the expression levels of several RAC‑α serine/threonine kinase (Akt) phosphorylation‑associated proteins and apoptosis‑regulating proteins were evaluated by western blot analysis. It was observed that the combination of cecropinXJ and LY294002 resulted in significant synergistic cytotoxic and apoptosis effects, as compared with any single agent alone, in a dose‑dependent manner. Corresponding to enhanced apoptosis, the expression levels of certain apoptosis‑regulating proteins were changed, the most notable being the upregulation of caspase‑3, B‑cell lymphoma‑2 (Bcl‑2)‑associated death promotor, Bcl‑2 homologous antagonist killer, Bcl‑2 interacting killer, Bcl‑2‑like protein 11, Bcl‑2‑like protein 4 and cytochrome c, and the downregulation of phosphorylated‑Bad and Bcl‑2 proteins. The present study provided a novel therapeutic regimen for the use of the cecropinXJ in combination with LY294002 for the treatment of gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xia LJ, Wu YL and Zhang FC: Combination of cecropinXJ and LY294002 induces synergistic cytotoxicity, and apoptosis in human gastric cancer cells via inhibition of the PI3K/Akt signaling pathway. Oncol Lett 14: 7522-7528, 2017.
APA
Xia, L., Wu, Y., & Zhang, F. (2017). Combination of cecropinXJ and LY294002 induces synergistic cytotoxicity, and apoptosis in human gastric cancer cells via inhibition of the PI3K/Akt signaling pathway. Oncology Letters, 14, 7522-7528. https://doi.org/10.3892/ol.2017.7112
MLA
Xia, L., Wu, Y., Zhang, F."Combination of cecropinXJ and LY294002 induces synergistic cytotoxicity, and apoptosis in human gastric cancer cells via inhibition of the PI3K/Akt signaling pathway". Oncology Letters 14.6 (2017): 7522-7528.
Chicago
Xia, L., Wu, Y., Zhang, F."Combination of cecropinXJ and LY294002 induces synergistic cytotoxicity, and apoptosis in human gastric cancer cells via inhibition of the PI3K/Akt signaling pathway". Oncology Letters 14, no. 6 (2017): 7522-7528. https://doi.org/10.3892/ol.2017.7112
Copy and paste a formatted citation
x
Spandidos Publications style
Xia LJ, Wu YL and Zhang FC: Combination of cecropinXJ and LY294002 induces synergistic cytotoxicity, and apoptosis in human gastric cancer cells via inhibition of the PI3K/Akt signaling pathway. Oncol Lett 14: 7522-7528, 2017.
APA
Xia, L., Wu, Y., & Zhang, F. (2017). Combination of cecropinXJ and LY294002 induces synergistic cytotoxicity, and apoptosis in human gastric cancer cells via inhibition of the PI3K/Akt signaling pathway. Oncology Letters, 14, 7522-7528. https://doi.org/10.3892/ol.2017.7112
MLA
Xia, L., Wu, Y., Zhang, F."Combination of cecropinXJ and LY294002 induces synergistic cytotoxicity, and apoptosis in human gastric cancer cells via inhibition of the PI3K/Akt signaling pathway". Oncology Letters 14.6 (2017): 7522-7528.
Chicago
Xia, L., Wu, Y., Zhang, F."Combination of cecropinXJ and LY294002 induces synergistic cytotoxicity, and apoptosis in human gastric cancer cells via inhibition of the PI3K/Akt signaling pathway". Oncology Letters 14, no. 6 (2017): 7522-7528. https://doi.org/10.3892/ol.2017.7112
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