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Article

Inhibition of breast cancer cell growth by the Pteris semipinnata extract ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic‑acid

  • Authors:
    • Juekun Wu
    • Lili Meng
    • Meijun Long
    • Ying Ruan
    • Xi Li
    • Yong Huang
    • Wanshou Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Thyroid and Breast Surgery, The Third Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China, Department of Gynecology and Obstetrics, The Sun Yat‑Sen Memorial Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China
  • Pages: 6809-6814
    |
    Published online on: October 2, 2017
       https://doi.org/10.3892/ol.2017.7113
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Abstract

Previous studies have demonstrated strong anti‑tumor effects of ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic‑acid (5F), an extract from Pteris semipinnata, in liver, lung, stomach and anaplastic thyroid cancer cells. However, whether 5F inhibits the growth of breast cancer cells remains unclear. The present study assessed the effect of 5F on breast cancer cells. The breast cancer cell lines MCF‑7, MDA‑MB‑231 and SK‑BR‑3 were each treated with 0, 5, 10, 20 and 40 µg/ml 5F. Morphological changes in the breast cancer cells were assessed using fluorescence microscopy. The proliferation and apoptosis of the breast cancer cells were also examined using Cell Counting Kit‑8 and flow cytometry. The levels of B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X apoptosis regulator (Bax), Bcl‑2 antagonist/killer (Bak) 1 and caspase‑3 in the breast cancer cells were assessed. The results of the present study demonstrated that 5F inhibited the proliferation of MCF‑7, MDA‑MB‑231 and SK‑BR‑3 breast cancer cells in a concentration‑ and time‑dependent manner. Treatment with 5F also induced the apoptosis of breast cancer cells. MDA‑MB‑231, MCF‑7, and SK‑BR‑3 cells exhibited apoptotic rates of 40.13, 60.44, and 70.49%, respectively, following incubation with 5F for 24 h. Furthermore, 5F significantly decreased the expression of Bcl‑2 and increased the expression of Bax, Bak, and caspase‑3 in a concentration‑dependent manner. The results of the present study revealed that the P. semipinnata extract 5F inhibited the growth of human breast cancer cells in a time‑ and concentration‑dependent manner, and that 5F induced apoptosis of human breast cancer cells.
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1 

Benson JR and Jatoi I: The global breast cancer burden. Future Oncol. 8:697–702. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Nandy A, Gangopadhyay S and Mukhopadhyay A: Individualizing breast cancer treatment-The dawn of personalized medicine. Exp Cell Res. 320:1–11. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Slamon DJ, Leyland-Jones B, Shak S, Fuchs H, Paton V, Bajamonde A, Fleming T, Eiermann W, Wolter J, Pegram M, et al: Use of chemotherapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2. N Engl J Med. 344:783–792. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Lim E and Winer EP: Adjuvant chemotherapy in luminal breast cancers. Breast. 20 Suppl 3:S128–131. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Liu ZM, Chen GG, Vlantis AC, Liang NC, Deng YF and van Hasselt CA: Cell death induced by ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid in anaplastic thyroid carcinoma cells is via a mitochondrial-mediated pathway. Apoptosis. 10:1345–1356. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Brunocolmenarez J, Peña A, Alarcón L, Usubillaga A and Delgadoméndez P: Structure of ent-15a-hydroxy-kaur-16-en-19-oic acid. Avances En Química. 6:16–20. 2011.

7 

Li L, Chen GG, Lu YN, Liu Y, Wu KF, Gong XL, Gou ZP, Li MY and Liang NC: Ent-11α-Hydroxy-15-oxo-kaur-16-en-19-oic-acid inhibits growth of human lung cancer A549 cells by arresting cell cycle and triggering apoptosis. Chin J Cancer Res. 24:109–115. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Chen GG, Leung J, Liang NC, Li L, Wu K, Chan UP, Leung BC, Li M, Du J, Deng YF, et al: Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid inhibits hepatocellular carcinoma in vitro and in vivo via stabilizing IkBα. Invest New Drugs. 30:2210–2218. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Liu Z, Ng EK, Liang NC, Deng YF, Leung BC and Chen GG: Cell death induced by Pteris semipinnata L. Is associated with p53 and oxidant stress in gastric cancer cells. FEBS Lett. 579:1477–1487. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Chen GG, Liang NC, Lee JF, Chan UP, Wang SH, Leung BC and Leung KL: Over-expression of Bcl-2 against Pteris semipinnata L-induced apoptosis of human colon cancer cells via a NF-kappa B-related pathway. Apoptosis. 9:619–627. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Vlantis AC, Lo CS, Chen GG, Liang Ci N, Lui VW, Wu K, Deng YF, Gong X, Lu Y, Tong MC and van Hasselt CA: Induction of laryngeal cancer cell death by Ent-11-hydroxy-15-oxo-kaur-16-en-19-oic acid. Head Neck. 32:1506–1518. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Mgbonyebi OP, Russo J and Russo IH: Roscovitine induces cell death and morphological changes indicative of apoptosis in MDA-MB-231 breast cancer cells. Cancer Res. 59:1903–1910. 1999.PubMed/NCBI

13 

Chen JF, Chen YX, Li P, Fu M, Lv YN and Li L: Effect of Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid on human gastric cancer cells and its mechanism. Nan Fang Yi Ke Da Xue Xue Bao. 31:1345–1348. 2011.(In Chinese). PubMed/NCBI

14 

Crépin M, Salle V, Raux H, Berger R, Hamelin R, Brouty-Boyé D and Israel L: Steroid hormone receptors and tumorigenicity of sublines from breast tumor metastatic MDA-MB 231 cell line. Anticancer Res. 10:1661–1666. 1990.PubMed/NCBI

15 

Duncan RE and Archer MC: Farnesol induces thyroid hormone receptor (THR) beta1 but inhibits THR-mediated signaling in MCF-7 human breast cancer cells. Biochem Biophys Res Commun. 343:239–243. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Reed JC: Dysregulation of apoptosis in cancer. J Clin Oncol. 17:2941–2953. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Gupta S: Molecular steps of death receptor and mitochondrial pathways of apoptosis. Life Sci. 69:2957–2964. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Pinton P, Ferrari D, Rapizzi E, Di Virgilio F, Pozzan T and Rizzuto R: The Ca2+ concentration of the endoplasmic reticulum is a key determinant of ceramide-induced apoptosis: Significance for the molecular mechanism of Bcl-2 action. EMBO J. 20:2690–2701. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Gross A, McDonnell JM and Korsmeyer SJ: BCL-2 family members and the mitochondria in apoptosis. Genes Dev. 13:1899–1911. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Eldering E, Mackus WJ, Derks IA, Evers LM, Beuling E, Teeling P, Lens SM, van Oers MH and van Lier RA: Apoptosis via the B cell antigen receptor requires Bax translocation and involves mitochondrial depolarization, cytochrome C release, and caspase-9 activation. Eur J Immunol. 34:1950–1960. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Chen F, Jiang X, Chen X, Liu G and Ding J: Effects of downregulation of inhibin alpha gene expression on apoptosis and proliferation of goose granulosa cells. J Genet Genomics. 34:1106–1113. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Pilchova I, Klacanova K, Chomova M, Tatarkova Z, Dobrota D and Racay P: Possible contribution of proteins of Bcl-2 family in neuronal death following transient global brain ischemia. Cell Mol Neurobiol. 35:23–31. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Guo B, Zhai D, Cabezas E, Welsh K, Nouraini S, Satterthwait AC and Reed JC: Humanin peptide suppresses apoptosis by interfering with Bax activation. Nature. 423:456–461. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Li MY, Liang NC and Chen GG: Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid induces apoptosis of human malignant cancer cells. Curr Drug Targets. 13:1730–1737. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu J, Meng L, Long M, Ruan Y, Li X, Huang Y and Qiu W: Inhibition of breast cancer cell growth by the Pteris semipinnata extract ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic‑acid. Oncol Lett 14: 6809-6814, 2017.
APA
Wu, J., Meng, L., Long, M., Ruan, Y., Li, X., Huang, Y., & Qiu, W. (2017). Inhibition of breast cancer cell growth by the Pteris semipinnata extract ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic‑acid. Oncology Letters, 14, 6809-6814. https://doi.org/10.3892/ol.2017.7113
MLA
Wu, J., Meng, L., Long, M., Ruan, Y., Li, X., Huang, Y., Qiu, W."Inhibition of breast cancer cell growth by the Pteris semipinnata extract ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic‑acid". Oncology Letters 14.6 (2017): 6809-6814.
Chicago
Wu, J., Meng, L., Long, M., Ruan, Y., Li, X., Huang, Y., Qiu, W."Inhibition of breast cancer cell growth by the Pteris semipinnata extract ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic‑acid". Oncology Letters 14, no. 6 (2017): 6809-6814. https://doi.org/10.3892/ol.2017.7113
Copy and paste a formatted citation
x
Spandidos Publications style
Wu J, Meng L, Long M, Ruan Y, Li X, Huang Y and Qiu W: Inhibition of breast cancer cell growth by the Pteris semipinnata extract ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic‑acid. Oncol Lett 14: 6809-6814, 2017.
APA
Wu, J., Meng, L., Long, M., Ruan, Y., Li, X., Huang, Y., & Qiu, W. (2017). Inhibition of breast cancer cell growth by the Pteris semipinnata extract ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic‑acid. Oncology Letters, 14, 6809-6814. https://doi.org/10.3892/ol.2017.7113
MLA
Wu, J., Meng, L., Long, M., Ruan, Y., Li, X., Huang, Y., Qiu, W."Inhibition of breast cancer cell growth by the Pteris semipinnata extract ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic‑acid". Oncology Letters 14.6 (2017): 6809-6814.
Chicago
Wu, J., Meng, L., Long, M., Ruan, Y., Li, X., Huang, Y., Qiu, W."Inhibition of breast cancer cell growth by the Pteris semipinnata extract ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic‑acid". Oncology Letters 14, no. 6 (2017): 6809-6814. https://doi.org/10.3892/ol.2017.7113
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