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Article

TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils

  • Authors:
    • Bing Zhang
    • Chun‑Yan Wei
    • Kai‑Kai Chang
    • Jia‑Jun Yu
    • Wen‑Jie Zhou
    • Hui‑Li Yang
    • Jun Shao
    • Jin‑Jin Yu
    • Ming‑Qing Li
    • Feng Xie
  • View Affiliations / Copyright

    Affiliations: Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai 200011, P.R. China, Department of Obstetrics and Gynecology, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, P.R. China, Medical Center of Diagnosis and Treatment for Cervical Diseases, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai 200011, P.R. China
  • Pages: 7483-7488
    |
    Published online on: October 3, 2017
       https://doi.org/10.3892/ol.2017.7121
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Abstract

Our previous study demonstrated that thymic stromal lymphopoietin (TSLP) secreted by cervical cancer cells promotes angiogenesis and recruitment, and regulates the function of eosinophils (EOS). However, the function of TSLP in the crosstalk between EOS and vascular endothelial cells in cancer lesions remains unknown. The aim of the present study was to investigate the effect of EOS caused by TSLP in in vitro angiogenesis of human umbilical vein endothelial cells (HUVECs). The results of the present study revealed that recombinant human TSLP protein (rhTSLP) increased the secretion of vascular endothelial growth factor (VEGF), but not fibroblast growth factors, in HL‑60‑eosinophils (HL‑60E). Compared with cervical cancer cells (HeLa or CasKi cells) or HL‑60E alone, there were increased levels of interleukin (IL)‑8 and VEGF in the co‑culture system between cervical cancer cells, and HL‑60E cells. This effect was strengthened by rhTSLP, but inhibited by inhibiting the TSLP signal with anti‑human TSLP or TSLP receptor neutralizing antibodies. The results of the tube formation assays revealed that treatment with the supernatant from cervical cancer cells and/or HL‑60E resulted in an increase in angiogenesis in HUVECs, which could be decreased by TSLP or TSLPR inhibitors. The results of the present study suggested that TSLP derived of cervical cancer cells may indirectly stimulate angiogenesis of HUVECs, by upregulating IL‑8 and VEGF production, in a co‑culture model between cervical cancer cells and EOS, therefore promoting the development of cervical cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang B, Wei CY, Chang KK, Yu JJ, Zhou WJ, Yang HL, Shao J, Yu JJ, Li MQ, Xie F, Xie F, et al: TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils. Oncol Lett 14: 7483-7488, 2017.
APA
Zhang, B., Wei, C., Chang, K., Yu, J., Zhou, W., Yang, H. ... Xie, F. (2017). TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils. Oncology Letters, 14, 7483-7488. https://doi.org/10.3892/ol.2017.7121
MLA
Zhang, B., Wei, C., Chang, K., Yu, J., Zhou, W., Yang, H., Shao, J., Yu, J., Li, M., Xie, F."TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils". Oncology Letters 14.6 (2017): 7483-7488.
Chicago
Zhang, B., Wei, C., Chang, K., Yu, J., Zhou, W., Yang, H., Shao, J., Yu, J., Li, M., Xie, F."TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils". Oncology Letters 14, no. 6 (2017): 7483-7488. https://doi.org/10.3892/ol.2017.7121
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang B, Wei CY, Chang KK, Yu JJ, Zhou WJ, Yang HL, Shao J, Yu JJ, Li MQ, Xie F, Xie F, et al: TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils. Oncol Lett 14: 7483-7488, 2017.
APA
Zhang, B., Wei, C., Chang, K., Yu, J., Zhou, W., Yang, H. ... Xie, F. (2017). TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils. Oncology Letters, 14, 7483-7488. https://doi.org/10.3892/ol.2017.7121
MLA
Zhang, B., Wei, C., Chang, K., Yu, J., Zhou, W., Yang, H., Shao, J., Yu, J., Li, M., Xie, F."TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils". Oncology Letters 14.6 (2017): 7483-7488.
Chicago
Zhang, B., Wei, C., Chang, K., Yu, J., Zhou, W., Yang, H., Shao, J., Yu, J., Li, M., Xie, F."TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils". Oncology Letters 14, no. 6 (2017): 7483-7488. https://doi.org/10.3892/ol.2017.7121
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