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Article Open Access

Effect of fraxetin on proliferation and apoptosis in breast cancer cells

  • Authors:
    • Guodong Liu
    • Zhenfang Liu
    • Yuexiang Yan
    • Haiyan Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Yidu Central Hospital of Weifang, Weifang, Shandong 262500, P.R. China, Department of General Surgery, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7374-7378
    |
    Published online on: October 5, 2017
       https://doi.org/10.3892/ol.2017.7143
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Abstract

The aim of the present study was to examine the effect of fraxetin on proliferation and apoptosis in the MCF-7 breast cancer cell line. Cell proliferation was measused using an MTT assay and 4’,6-diamidino-2-phenylindole (DAPI) staining was used to determine shrinkage and condensation. RT-PCR was used to examine the expression of factor‑associated suicide (Fas) and Fas ligand (FasL) mRNA, and western blot analysis was used to examine Bax and Bcl-2 protein. MTT showed that the proliferation of MCF-7 cells was significantly inhibited by fraxetin in a dose-dependent manner. Fraxetin also induced significant morphological changes of MCF-7 cells, suggestive of apoptosis, whereas DAPI staining showed that fraxetin caused cell shrinkage and chromatin condensation. RT-PCR showed that the expression of Fas and FasL mRNA was upregulated by fraxetin and the western blot analysis revealed that Bax was upregulated and Bcl-2 was downregulated. In conclusion, fraxetin can inhibit the proliferation of MCF-7 cells, induce apoptosis, upregulate Fas, FasL and Bax, and downregulate Bcl-2 to induce apoptosis. These results support the potential therapeutic role for fraxetin in breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liu G, Liu Z, Yan Y and Wang H: Effect of fraxetin on proliferation and apoptosis in breast cancer cells. Oncol Lett 14: 7374-7378, 2017.
APA
Liu, G., Liu, Z., Yan, Y., & Wang, H. (2017). Effect of fraxetin on proliferation and apoptosis in breast cancer cells. Oncology Letters, 14, 7374-7378. https://doi.org/10.3892/ol.2017.7143
MLA
Liu, G., Liu, Z., Yan, Y., Wang, H."Effect of fraxetin on proliferation and apoptosis in breast cancer cells". Oncology Letters 14.6 (2017): 7374-7378.
Chicago
Liu, G., Liu, Z., Yan, Y., Wang, H."Effect of fraxetin on proliferation and apoptosis in breast cancer cells". Oncology Letters 14, no. 6 (2017): 7374-7378. https://doi.org/10.3892/ol.2017.7143
Copy and paste a formatted citation
x
Spandidos Publications style
Liu G, Liu Z, Yan Y and Wang H: Effect of fraxetin on proliferation and apoptosis in breast cancer cells. Oncol Lett 14: 7374-7378, 2017.
APA
Liu, G., Liu, Z., Yan, Y., & Wang, H. (2017). Effect of fraxetin on proliferation and apoptosis in breast cancer cells. Oncology Letters, 14, 7374-7378. https://doi.org/10.3892/ol.2017.7143
MLA
Liu, G., Liu, Z., Yan, Y., Wang, H."Effect of fraxetin on proliferation and apoptosis in breast cancer cells". Oncology Letters 14.6 (2017): 7374-7378.
Chicago
Liu, G., Liu, Z., Yan, Y., Wang, H."Effect of fraxetin on proliferation and apoptosis in breast cancer cells". Oncology Letters 14, no. 6 (2017): 7374-7378. https://doi.org/10.3892/ol.2017.7143
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