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MiR‑124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6

  • Authors:
    • Li Yuan
    • Shaolin Li
    • Qi Zhou
    • Dong Wang
    • Dongling Zou
    • Jin Shu
    • Yu Huang
  • View Affiliations / Copyright

    Affiliations: Department of Radiological Medicine, Chongqing Medical University, Chongqing 400016, P.R. China, Department of Gynecologic Oncology, Chongqing Cancer Institute, Chongqing 400030, P.R. China
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7311-7317
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    Published online on: October 10, 2017
       https://doi.org/10.3892/ol.2017.7157
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Abstract

Epithelial ovarian cancer remains the most common type of malignant tumor of the female reproductive system worldwide. Routine surgery and chemotherapy are the best treatments available for patients with ovarian cancer; however, almost 40% of ovarian cancer cases are intractable, with poor 5‑year survival rates. MicroRNAs (miRNA) are endogenous small non‑coding RNA molecules that function in transcriptional and post‑transcriptional regulation of gene expression in various cellular processes. Recent studies demonstrated that microRNA (miR)‑124 was downregulated in numerous types of tumors; however, the function and mechanism underlying miR‑124 in epithelial ovarian cancer remain unclear. The present study revealed that miR‑124 may be significantly downregulated in epithelial ovarian cancer. Using prediction algorithms and luciferase reporter gene assays, the present study identified and confirmed programmed cell death 6 (PDCD6) as a novel, direct target of miR‑124. Overexpression of miR‑124 suppressed PDCD6 expression, inhibited cell proliferation, migration and invasion, and induced apoptosis in SKOV3 and OCVAR3 cells in vitro. In the present study, overexpression of PDCD6 in epithelial ovarian cancer cells co‑transfected with miR‑124 effectively reversed the miR‑124‑induced apoptosis. Therefore, the results of the present study suggested that miR‑124 is a tumor suppressor miRNA and a potential target for future treatment of ovarian malignant neoplasms.
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1 

Farazi TA, Hoell JI, Morozov P and Tuschl T: MicroRNAs in human cancer. Adv Exp Med Biol. 774:1–20. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, Ebert BL, Mak RH, Ferrando AA, et al: MicroRNA expression profiles classify human cancers. Nature. 435:834–838. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Iorio MV and Croce CM: MicroRNAs in cancer: Small molecules with a huge impact. J Clin Oncol. 27:5848–5856. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Iorio MV, Visone R, Di Leva G, Donati V, Petrocca F, Casalini P, Taccioli C, Volinia S, Liu CG, Alder H, et al: MicroRNA signatures in human ovarian cancer. Cancer Res. 67:8699–8707. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Abba M, Mudduluru G and Allgayer H: MicroRNAs in cancer: Small molecules, big chances. Anticancer Agents Med Chem. 12:733–743. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Gandellini P, Giovannetti E and Nicassio F: MicroRNAs in cancer management: Big challenges for small molecules. Biomed Res Int. 2015:9821562015. View Article : Google Scholar : PubMed/NCBI

7 

Calin GA, Sevignani C, Dumitru CD, Hyslop T, Noch E, Yendamuri S, Shimizu M, Rattan S, Bullrich F, Negrini M and Croce CM: Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci USA. 101:pp. 2999–3004. 2004, View Article : Google Scholar : PubMed/NCBI

8 

Kim K, Zang R, Choi SC, Ryu SY and Kim JW: Current status of gynecological cancer in China. J Gynecol Oncol. 20:72–76. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Lees B and Leath CA III: The impact of diabetes on gynecologic cancer: Current status and future directions. Curr Obstet Gynecol Rep. 4:234–239. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Pal MK, Jaiswar SP, Dwivedi VN, Tripathi AK, Dwivedi A and Sankhwar P: MicroRNA: A new and promising potential biomarker for diagnosis and prognosis of ovarian cancer. Cancer Biol Med. 12:328–341. 2015.PubMed/NCBI

11 

Langhe R: microRNA and ovarian cancer. Adv Exp Med Biol. 889:119–151. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Maalouf SW, Liu WS and Pate JL: MicroRNA in ovarian function. Cell Tissue Res. 363:7–18. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Zaman MS, Maher DM, Khan S, Jaggi M and Chauhan SC: Current status and implications of microRNAs in ovarian cancer diagnosis and therapy. J Ovarian Res. 5:442012. View Article : Google Scholar : PubMed/NCBI

14 

Zhang Y, Zheng L, Huang J, Gao F, Lin X, He L, Li D, Li Z, Ding Y and Chen L: MiR-124 Radiosensitizes human colorectal cancer cells by targeting PRRX1. PLoS One. 9:e939172014. View Article : Google Scholar : PubMed/NCBI

15 

Zhang W, Mao YQ, Wang H, Yin WJ, Zhu SX and Wang WC: MiR-124 suppresses cell motility and adhesion by targeting talin 1 in prostate cancer cells. Cancer Cell Int. 15:492015. View Article : Google Scholar : PubMed/NCBI

16 

Lu Y, Yue X, Cui Y, Zhang J and Wang K: MicroRNA-124 suppresses growth of human hepatocellular carcinoma by targeting STAT3. Biochem Biophys Res Commun. 441:873–879. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Zheng YB, Xiao GC, Tong SL, Ding Y, Wang QS, Li SB and Hao ZN: Paeoniflorin inhibits human gastric carcinoma cell proliferation through up-regulation of microRNA-124 and suppression of PI3K/Akt and STAT3 signaling. World J Gastroenterol. 21:7197–7207. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Vito P, Lacanà E and D'Adamio L: Interfering with apoptosis: Ca(2+)-binding protein ALG-2 and Alzheimer's disease gene ALG-3. Science. 271:521–525. 1996. View Article : Google Scholar : PubMed/NCBI

19 

la Cour JM, Høj BR, Mollerup J, Simon R, Sauter G and Berchtold MW: The apoptosis linked gene ALG-2 is dysregulated in tumors of various origin and contributes to cancer cell viability. Mol Oncol. 1:431–439. 2008. View Article : Google Scholar : PubMed/NCBI

20 

D'Adamio L, Lacanà E and Vito P: Functional cloning of genes involved in T-cell receptor-induced programmed cell death. Semin Immunol. 9:17–23. 1997. View Article : Google Scholar : PubMed/NCBI

21 

Mahul-Mellier AL, Strappazzon F, Petiot A, Chatellard-Causse C, Torch S, Blot B, Freeman K, Kuhn L, Garin J, Verna JM, et al: Alix and ALG-2 are involved in tumor necrosis factor receptor 1-induced cell death. J Biol Chem. 283:34954–34965. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Giulietti A, Overbergh L, Valckx D, Decallonne B, Bouillon R and Mathieu C: An overview of real-time quantitative PCR: Applications to quantify cytokine gene expression. Methods. 25:386–401. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Dwivedi SK, Mustafi SB, Mangala LS, Jiang D, Pradeep S, Rodriguez-Aguayo C, Ling H, Ivan C, Mukherjee P, Calin GA, et al: Therapeutic evaluation of microRNA-15a and microRNA-16 in ovarian cancer. Oncotarget. 7:15093–15104. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Cheng CJ, Bahal R, Babar IA, Pincus Z, Barrera F, Liu C, Svoronos A, Braddock DT, Glazer PM, Engelman DM, et al: MicroRNA silencing for cancer therapy targeted to the tumour microenvironment. Nature. 518:107–110. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Maltby S, Plank M, Tay HL, Collison A and Foster PS: Targeting MicroRNA function in respiratory diseases: Mini-review. Front Physiol. 7:212016. View Article : Google Scholar : PubMed/NCBI

26 

Zhang H, Wang Q, Zhao Q and Di W: MiR-124 inhibits the migration and invasion of ovarian cancer cells by targeting SphK1. J Ovarian Res. 6:842013. View Article : Google Scholar : PubMed/NCBI

27 

la Cour JM, Mollerup J, Winding P, Tarabykina S, Sehested M and Berchtold MW: Up-regulation of ALG-2 in hepatomas and lung cancer tissue. Am J Pathol. 163:81–89. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Su D, Xu H, Feng J, Gao Y, Gu L, Ying L, Katsaros D, Yu H, Xu S and Qi M: PDCD6 is an independent predictor of progression free survival in epithelial ovarian cancer. J Transl Med. 10:312012. View Article : Google Scholar : PubMed/NCBI

29 

Jung YS, Kim KS, Kim KD, Lim JS, Kim JW and Kim E: Apoptosis-linked gene 2 binds to the death domain of Fas and dissociates from Fas during Fas-mediated apoptosis in Jurkat cells. Biochem Biophys Res Commun. 288:420–426. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Zhou B, Bai P, Xue H, Zhang Z, Shi S, Zhang K, Wang Y, Wang K, Quan Y, Song Y and Zhang L: Single nucleotide polymorphisms in PDCD6 gene are associated with the development of cervical squamous cell carcinoma. Fam Cancer. 14:1–8. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Jang IK, Hu R, Lacaná E, D'Adamio L and Gu H: Apoptosis-linked gene 2-deficient mice exhibit normal T-cell development and function. Mol Cell Biol. 22:4094–4100. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Aviel-Ronen S, Coe BP, Lau SK, da Cunha Santos G, Zhu CQ, Strumpf D, Jurisica I, Lam WL and Tsao MS: Genomic markers for malignant progression in pulmonary adenocarcinoma with bronchioloalveolar features. Proc Natl Acad Sci USA. 105:pp. 10155–10160. 2008, View Article : Google Scholar : PubMed/NCBI

33 

Mahul-Mellier AL, Hemming FJ, Blot B, Fraboulet S and Sadoul R: Alix, making a link between apoptosis-linked gene-2, the endosomal sorting complexes required for transport, and neuronal death in vivo. J Neurosci. 26:542–549. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Suzuki K, Dashzeveg N, Lu ZG, Taira N, Miki Y and Yoshida K: Programmed cell death 6, a novel p53-responsive gene, targets to the nucleus in the apoptotic response to DNA damage. Cancer Sci. 103:1788–1794. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Høj BR, la Cour JM, Mollerup J and Berchtold MW: ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death. Biochem Biophys Res Commun. 378:145–148. 2009. View Article : Google Scholar : PubMed/NCBI

36 

la Cour JM, Mollerup J, Winding P, Tarabykina S, Sehested M and Berchtold MW: Up-regulation of ALG-2 cancer tissue in hepatomas and lung. Am J Pathol. 163:81–89. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yuan L, Li S, Zhou Q, Wang D, Zou D, Shu J and Huang Y: MiR‑124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6. Oncol Lett 14: 7311-7317, 2017.
APA
Yuan, L., Li, S., Zhou, Q., Wang, D., Zou, D., Shu, J., & Huang, Y. (2017). MiR‑124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6. Oncology Letters, 14, 7311-7317. https://doi.org/10.3892/ol.2017.7157
MLA
Yuan, L., Li, S., Zhou, Q., Wang, D., Zou, D., Shu, J., Huang, Y."MiR‑124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6". Oncology Letters 14.6 (2017): 7311-7317.
Chicago
Yuan, L., Li, S., Zhou, Q., Wang, D., Zou, D., Shu, J., Huang, Y."MiR‑124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6". Oncology Letters 14, no. 6 (2017): 7311-7317. https://doi.org/10.3892/ol.2017.7157
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan L, Li S, Zhou Q, Wang D, Zou D, Shu J and Huang Y: MiR‑124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6. Oncol Lett 14: 7311-7317, 2017.
APA
Yuan, L., Li, S., Zhou, Q., Wang, D., Zou, D., Shu, J., & Huang, Y. (2017). MiR‑124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6. Oncology Letters, 14, 7311-7317. https://doi.org/10.3892/ol.2017.7157
MLA
Yuan, L., Li, S., Zhou, Q., Wang, D., Zou, D., Shu, J., Huang, Y."MiR‑124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6". Oncology Letters 14.6 (2017): 7311-7317.
Chicago
Yuan, L., Li, S., Zhou, Q., Wang, D., Zou, D., Shu, J., Huang, Y."MiR‑124 inhibits invasion and induces apoptosis of ovarian cancer cells by targeting programmed cell death 6". Oncology Letters 14, no. 6 (2017): 7311-7317. https://doi.org/10.3892/ol.2017.7157
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