Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
December-2017 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2017 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1

  • Authors:
    • Zhensong Yue
    • Tongguo Si
    • Zhanyu Pan
    • Wenfeng Cao
    • Zhuchen Yan
    • Zhansheng Jiang
    • Huaqiang Ouyang
  • View Affiliations / Copyright

    Affiliations: Department of Integrated Chinese and Western Medicine, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China, Department of Invasive Technology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China, Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China
  • Pages: 7941-7946
    |
    Published online on: October 19, 2017
       https://doi.org/10.3892/ol.2017.7224
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Sophoridine is an alkaloid extracted from Sophora alopecuroides that has extensive pharmacological actions. In the present study, the effect of sophoridine on cell growth of human medulloblastoma and its mechanism were investigated. Human medulloblastoma D283‑Med cells were incubated with 0, 0.5, 1 or 2 mg/ml sophoridine for 24, 48 or 72 h. Cell proliferation and cytotoxicity were analyzed using MTT and lactate dehydrogenase assays, respectively. Next, analyses of cell apoptosis and caspase‑3/8 activity were performed using flow cytometry or spectrophotometry, respectively. Lastly, the change in FoxM1, TrkB, BDNF, NF‑κB and AP‑1 expression was investigated using western blot analysis. In the present study, treatment with sophoridine significantly suppressed cell growth and induced apoptosis in human medulloblastoma cells. In addition, sophoridine significantly increased cytotoxicity and caspase‑3/8 activity in human medulloblastoma. Finally, it was found that sophoridine suppresses the protein expression of FoxM1, TrkB, BDNF NF‑κB and AP‑1 in human medulloblastoma cells. The present study suggests that sophoridine suppresses cell growth of human medulloblastoma through the inhibition of the FoxM1, NF‑κB and AP‑1 signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

View References

1 

Robinson GW, Orr BA, Wu G, Gururangan S, Lin T, Qaddoumi I, Packer RJ, Goldman S, Prados MD, Desjardins A, et al: Vismodegib exerts targeted efficacy against recurrent sonic hedgehog-subgroup medulloblastoma: Results from phase ii pediatric brain tumor consortium studies PBTC-025B and PBTC-032. J Clin Oncol. 33:2646–2654. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Deutsch M, Thomas PR, Krischer J, Boyett JM, Albright L, Aronin P, Langston J, Allen JC, Packer RJ, Linggood R, et al: Results of a prospective randomized trial comparing standard dose neuraxis irradiation (3,600 cGy/20) with reduced neuraxis irradiation (2,340 cGy/13) in patients with low-stage medulloblastoma. A combined children's cancer group-pediatric oncology group study. Pediatr Neurosurg. 24:167–177. 1996. View Article : Google Scholar : PubMed/NCBI

3 

Allen J, Donahue B, Mehta M, Miller DC, Rorke LB, Jakacki R, Robertson P, Sposto R, Holmes E, Vezina G, et al: A phase II study of preradiotherapy chemotherapy followed by hyperfractionated radiotherapy for newly diagnosed high-risk medulloblastoma/primitive neuroectodermal tumor: A report from the children's oncology group (CCG 9931). Int J Radiat Oncol Biol Phys. 74:1006–1011. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Cefalo G, Massimino M, Ruggiero A, Barone G, Ridola V, Spreafico F, Potepan P, Abate ME, Mascarin M, Garrè ML, et al: Temozolomide is an active agent in children with recurrent medulloblastoma/primitive neuroectodermal tumor: An Italian multi-institutional phase II trial. Neuro Oncol. 16:748–753. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Dhall G, Grodman H, Ji L, Gardner S, Dunkel IJ, McCowage GB, Diez B, Allen JC, Gopalan A, Cornelius AS, et al: Outcome of children less than three years old at diagnosis with non-metastatic medulloblastoma treated with chemotherapy on the ‘Head Start’ I and II protocols. Pediatr Blood Cancer. 50:1169–1175. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Ito T, Kohashi K, Yamada Y, Maekawa A, Kuda M, Furue M and Oda Y: Prognostic significance of Forkhead box M1 (FOXM1) expression and antitumor effect of FOXM1 inhibition in melanoma. Histopathology. 69:63–71. 2015. View Article : Google Scholar

7 

Dai J, Yang L, Wang J, Xiao Y and Ruan Q: Prognostic value of FOXM1 in patients with malignant solid tumor: A meta-analysis and system review. Dis Markers. 2015:3524782015. View Article : Google Scholar : PubMed/NCBI

8 

Zhao F and Lam EW: Role of the forkhead transcription factor FOXO-FOXM1 axis in cancer and drug resistance. Front Med. 6:376–380. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Quan M, Wang P, Cui J, Gao Y and Xie K: The roles of FOXM1 in pancreatic stem cells and carcinogenesis. Mol Cancer. 12:1592013. View Article : Google Scholar : PubMed/NCBI

10 

Shi M, Cui J and Xie K: Signaling of miRNAs-FOXM1 in cancer and potential targeted therapy. Curr Drug Targets. 14:1192–1202. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Chambers M, Kirkpatrick G, Evans M, Gorski G, Foster S and Borghaei RC: IL-4 inhibition of IL-1 induced Matrix metalloproteinase-3 (MMP-3) expression in human fibroblasts involves decreased AP-1 activation via negative crosstalk involving of Jun N-terminal kinase (JNK). Exp Cell Res. 319:1398–1408. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Uluckan O, Guinea-Viniegra J, Jimenez M and Wagner EF: Signalling in inflammatory skin disease by AP-1 (Fos/Jun). Clin Exp Rheumatol. 33 4 Suppl 92:S44–S49. 2015.PubMed/NCBI

13 

Wang WX, Sun ZH, Chen HM, Xu BN and Wang FY: Role and mechanism of Sophoridine on proliferation inhibition in human glioma U87MG cell line. Int J Clin Exp Med. 8:464–471. 2015.PubMed/NCBI

14 

Zhang B, Liu ZY, Li YY, Luo Y, Liu ML, Dong HY, Wang YX, Liu Y, Zhao PT, Jin FG and Li ZC: Antiinflammatory effects of matrine in LPS-induced acute lung injury in mice. Eur J Pharm Sci. 44:573–579. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Liou CJ, Lai YR, Chen YL, Chang YH, Li ZY and Huang WC: Matrine attenuates COX-2 and ICAM-1 Expressions in human lung epithelial cells and prevents acute lung injury in LPS-induced mice. Mediators Inflamm. 2016:36304852016. View Article : Google Scholar : PubMed/NCBI

16 

Gessi M, von Bueren AO, Rutkowski S and Pietsch T: p53 expression predicts dismal outcome for medulloblastoma patients with metastatic disease. J Neurooncol. 106:135–141. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Camara-Costa H, Resch A, Kieffer V, Lalande C, Poggi G, Kennedy C, Bull K, Calaminus G, Grill J, Doz F, et al: Neuropsychological outcome of children treated for standard risk Medulloblastoma in the PNET4 european randomized controlled trial of hyperfractionated versus standard radiation therapy and maintenance chemotherapy. Int J Radiat Oncol Biol Phys. 92:978–985. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Palmer SL, Leigh L, Ellison SC, Onar-Thomas A, Wu S, Qaddoumi I, Armstrong GT, Wright K, Wetmore C, Broniscer A and Gajjar A: Feasibility and efficacy of a computer-based intervention aimed at preventing reading decoding deficits among children undergoing active treatment for medulloblastoma: Results of a randomized trial. J Pediatr Psychol. 39:450–458. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Wang HQ, Jin JJ and Wang J: Matrine induces mitochondrial apoptosis in cisplatin-resistant non-small cell lung cancer cells via suppression of β-catenin/survivin signaling. Oncol Rep. 33:2561–2566. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Arora R, Yates C, Gary BD, McClellan S, Tan M, Xi Y, Reed E, Piazza GA, Owen LB and Dean-Colomb W: Panepoxydone targets NF-kB and FOXM1 to inhibit proliferation, induce apoptosis and reverse epithelial to mesenchymal transition in breast cancer. PLoS One. 9:e983702014. View Article : Google Scholar : PubMed/NCBI

21 

Arslan S, Korkmaz Ö, Özbilüm N and Berkan Ö: Association between NF-κBI and NF-κB BIA polymorphisms and coronary artery disease. Biomed Rep. 3:736–740. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Qian Y, Guan T, Huang M, Cao L, Li Y, Cheng H, Jin H and Yu D: Neuroprotection by the soy isoflavone, genistein, via inhibition of mitochondria-dependent apoptosis pathways and reactive oxygen induced-NF-κB activation in a cerebral ischemia mouse model. Neurochem Int. 60:759–767. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Li Q, Lai Y, Wang C, Xu G, He Z, Shang X, Sun Y, Zhang F, Liu L and Huang H: Matrine inhibits the proliferation, invasion and migration of castration-resistant prostate cancer cells through regulation of the NF-κB signaling pathway. Oncol Rep. 35:375–381. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Kasemeier-Kulesa JC, Morrison JA, Lefcort F and Kulesa PM: TrkB/BDNF signalling patterns the sympathetic nervous system. Nat Commun. 6:82812015. View Article : Google Scholar : PubMed/NCBI

25 

Ghiglieri V, Sgobio C, Patassini S, Bagetta V, Fejtova A, Giampà C, Marinucci S, Heyden A, Gundelfinger ED, Fusco FR, et al: TrkB/BDNF-dependent striatal plasticity and behavior in a genetic model of epilepsy: Modulation by valproic acid. Neuropsychopharmacology. 35:1531–1540. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Turner BA, Sparrow J, Cai B, Monroe J, Mikawa T and Hempstead BL: TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions. Dev Biol. 299:455–465. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Odate S, Nakamura K, Onishi H, Kojima M, Uchiyama A, Nakano K, Kato M, Tanaka M and Katano M: TrkB/BDNF signaling pathway is a potential therapeutic target for pulmonary large cell neuroendocrine carcinoma. Lung Cancer. 79:205–214. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Kan QC, Lv P, Zhang XJ, Xu YM, Zhang GX and Zhu L: Matrine protects neuro-axon from CNS inflammation-induced injury. Exp Mol Pathol. 98:124–130. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Xu L, Ning H, Gu L, Wang Q, Lu W, Peng H, Cui W, Ying B, Ross CR, Wilson GM, et al: Tristetraprolin induces cell cycle arrest in breast tumor cells through targeting AP-1/c-Jun and NF-κB pathway. Oncotarget. 6:41679–41691. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Kuang H, Hua X, Zhou J and Yang R: Resolvin D1 and E1 alleviate the progress of hepatitis toward liver cancer in long-term concanavalin A-induced mice through inhibition of NF-κB activity. Oncol Rep. 35:307–317. 2016. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yue Z, Si T, Pan Z, Cao W, Yan Z, Jiang Z and Ouyang H: Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1. Oncol Lett 14: 7941-7946, 2017.
APA
Yue, Z., Si, T., Pan, Z., Cao, W., Yan, Z., Jiang, Z., & Ouyang, H. (2017). Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1. Oncology Letters, 14, 7941-7946. https://doi.org/10.3892/ol.2017.7224
MLA
Yue, Z., Si, T., Pan, Z., Cao, W., Yan, Z., Jiang, Z., Ouyang, H."Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1". Oncology Letters 14.6 (2017): 7941-7946.
Chicago
Yue, Z., Si, T., Pan, Z., Cao, W., Yan, Z., Jiang, Z., Ouyang, H."Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1". Oncology Letters 14, no. 6 (2017): 7941-7946. https://doi.org/10.3892/ol.2017.7224
Copy and paste a formatted citation
x
Spandidos Publications style
Yue Z, Si T, Pan Z, Cao W, Yan Z, Jiang Z and Ouyang H: Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1. Oncol Lett 14: 7941-7946, 2017.
APA
Yue, Z., Si, T., Pan, Z., Cao, W., Yan, Z., Jiang, Z., & Ouyang, H. (2017). Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1. Oncology Letters, 14, 7941-7946. https://doi.org/10.3892/ol.2017.7224
MLA
Yue, Z., Si, T., Pan, Z., Cao, W., Yan, Z., Jiang, Z., Ouyang, H."Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1". Oncology Letters 14.6 (2017): 7941-7946.
Chicago
Yue, Z., Si, T., Pan, Z., Cao, W., Yan, Z., Jiang, Z., Ouyang, H."Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1". Oncology Letters 14, no. 6 (2017): 7941-7946. https://doi.org/10.3892/ol.2017.7224
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team