Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
December-2017 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2017 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Overexpression of NEDD9 in renal cell carcinoma is associated with tumor migration and invasion

  • Authors:
    • Jue Wang
    • Si Wang
    • Yun Luan
    • Wenhua Zhang
    • Chao Sun
    • Guanghui Cheng
    • Kailin Li
    • Qian Xin
    • Zhaomin Lin
    • Tonggang Qi
    • Feng Kong
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Neurology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China, Department of Urology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8021-8027
    |
    Published online on: October 20, 2017
       https://doi.org/10.3892/ol.2017.7231
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Scaffold protein neural precursor cell expressed, developmentally downregulated 9 (NEDD9) is a member of the Crk‑associated substrate protein family and is known to be a biomarker in multiple cancer types. It serves a critical function in regulating cell proliferation, migration, invasion and survival. The objective of this study was to evaluate the potential effects of NEDD9 in renal cell carcinoma (RCC). The expression of NEDD9 was analyzed by immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction. NEDD9 protein and mRNA levels were significantly upregulated in RCC tissues compared with normal tissues (P<0.001). Furthermore, the NEDD9 immunostaining level was significantly associated with primary tumor stage and tumor, node, metastasis stage (P<0.05). High NEDD9 expression resulted in significantly lower survival rates for patients compared with normal NEDD9 expression (P<0.01). In addition, wound healing and transwell assays indicated that NEDD9 depletion by small interfering RNA significantly attenuated the migration and invasion of RCC cells (P<0.001). The present data suggested that NEDD9 may be a novel target for prevention and treatment of RCC metastasis and recurrence.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Baldewijns MM, van Vlodrop IJ, Schouten LJ, Soetekouw PM, de Bruïne AP and van Engeland M: Genetics and epigenetics of renal cell cancer. Biochim Biophys Acta. 1785:133–155. 2008.PubMed/NCBI

3 

Drucker BJ: Renal cell carcinoma: Current status and future prospects. Cancer Treat Rev. 31:536–545. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Huang Y, Dai Y, Yang J, Chen T, Yin Y, Tang M, Hu C and Zhang L: Microarray analysis of microRNA expression in renal clear cell carcinoma. Eur J Surg Oncol. 35:1119–1123. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Shagisultanova E, Gaponova AV, Gabbasov R, Nicolas E and Golemis EA: Preclinical and clinical studies of the NEDD9 scaffold protein in cancer and other diseases. Gene. 567:1–11. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Kim M, Gans JD, Nogueira C, Wang A, Paik JH, Feng B, Brennan C, Hahn WC, Cordon-Cardo C, Wagner SN, et al: Comparative oncogenomics identifies NEDD9 as a melanoma metastasis gene. Cell. 125:1269–1281. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Natarajan M, Stewart JE, Golemis EA, Pugacheva EN, Alexandropoulos K, Cox BD, Wang W, Grammer JR and Gladson CL: HEF1 is a necessary and specific downstream effector of FAK that promotes the migration of glioblastoma cells. Oncogene. 25:1721–1732. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Tornillo G, Defilippi P and Cabodi S: Cas proteins: Dodgy scaffolding in breast cancer. Breast Cancer Res. 16:4432014. View Article : Google Scholar : PubMed/NCBI

9 

Loskutov YV, Kozyulina PY, Kozyreva VK, Ice RJ, Jones BC, Roston TJ, Smolkin MB, Ivanov AV, Wysolmerski RB and Pugacheva EN: NEDD9/Arf6-dependent endocytic trafficking of matrix metalloproteinase 14: A novel mechanism for blocking mesenchymal cell invasion and metastasis of breast cancer. Oncogene. 34:3662–3675. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Kondo S, Iwata S, Yamada T, Inoue Y, Ichihara H, Kichikawa Y, Katayose T, Souta-Kuribara A, Yamazaki H, Hosono O, et al: Impact of the integrin signaling adaptor protein NEDD9 on prognosis and metastatic behavior of human lung cancer. Clin Cancer Res. 18:6326–6338. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Wang J, Yang WJ, Sun C, Luan Y, Cheng GH, Li KL and Kong F: siRNA suppression of NEDD9 inhibits proliferation and enhances apoptosis in renal cell carcinoma. Oncol Res. 22:219–224. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Han T, Yi XP, Liu B, Ke MJ and Li YX: MicroRNA-145 suppresses cell proliferation, invasion and migration in pancreatic cancer cells by targeting NEDD9. Mol Med Rep. 11:4115–4120. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Izumchenko E, Singh MK, Plotnikova OV, Tikhmyanova N, Little JL, Serebriiskii IG, Seo S, Kurokawa M, Egleston BL, Klein-Szanto A, et al: NEDD9 promotes oncogenic signaling in mammary tumor development. Cancer Res. 69:7198–7206. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Eble J, Sauter G, Epstein J and Sesterhenn IE: World health organization classification of tumors pathology and genetics of the urinary systemandmale genital organs. LARC Press; Lyon: pp. 12–14. 2004

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-timequantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Tikhmyanova N, Tulin AV, Roegiers F and Golemis EA: Dcas supports cell polarization and cell-cell adhesion complexes in development. PLoS One. 5:e123692010. View Article : Google Scholar : PubMed/NCBI

17 

Nikonova AS, Gaponova AV, Kudinov AE and Golemis EA: CAS proteins in health and disease: An update. IUBMB Life. 66:387–395. 2014. View Article : Google Scholar : PubMed/NCBI

18 

O'Neill GM, Seo S, Serebriiskii IG, Lessin SR and Golemis EA: A new central scaffold for metastasis: Parsing HEF1/Cas-L/NEDD9. Cancer Res. 67:8975–8979. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Xue YZ, Sheng YY, Liu ZL, Wei ZQ, Cao HY, Wu YM, Lu YF, Yu LH, Li JP and Li ZS: Expression of NEDD9 in pancreatic ductal adenocarcinoma and its clinical significance. Tumour Biol. 34:895–899. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Li P, Zhou H, Zhu X, Ma G, Liu C, Lin B and Mao W: High expression of NEDD9 predicts adverse outcomes of colorectal cancer patients. Int J Clin Exp Pathol. 7:2565–2570. 2014.PubMed/NCBI

21 

Shi R, Wang L, Wang T, Xu J, Wang F and Xu M: NEDD9 overexpression correlates with the progression and prognosis in gastric carcinoma. Med Oncol. 31:8522014. View Article : Google Scholar : PubMed/NCBI

22 

Wan L, Pantel K and Kang Y: Tumor metastasis: Moving new biological insights into the clinic. Nat Med. 19:1450–1464. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Zeng G, Gao L and Yu RK: Reduced cell migration, tumor growth and experimental metastasis of rat F-11 cells whose expression of GD3-synthase is suppressed. Int J Cancer. 88:53–57. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Shin KD, Lee MY, Shin DS, Lee S, Son KH, Koh S, Paik YK, Kwon BM and Han DC: Blocking tumor cell migration and invasion with biphenyl isoxazole derivative KRIBB3, a synthetic molecule that inhibits Hsp27 phosphorylation. J Biol Chem. 280:41439–41448. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Feng J, Zhao J, Xie H, Yin Y, Luo G, Zhang J, Feng Y and Li Z: Involvement of NEDD9 in the invasion and migration of gastric cancer. Tumour Biol. 36:3621–3628. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang J, Wang S, Luan Y, Zhang W, Sun C, Cheng G, Li K, Xin Q, Lin Z, Qi T, Qi T, et al: Overexpression of NEDD9 in renal cell carcinoma is associated with tumor migration and invasion. Oncol Lett 14: 8021-8027, 2017.
APA
Wang, J., Wang, S., Luan, Y., Zhang, W., Sun, C., Cheng, G. ... Kong, F. (2017). Overexpression of NEDD9 in renal cell carcinoma is associated with tumor migration and invasion. Oncology Letters, 14, 8021-8027. https://doi.org/10.3892/ol.2017.7231
MLA
Wang, J., Wang, S., Luan, Y., Zhang, W., Sun, C., Cheng, G., Li, K., Xin, Q., Lin, Z., Qi, T., Kong, F."Overexpression of NEDD9 in renal cell carcinoma is associated with tumor migration and invasion". Oncology Letters 14.6 (2017): 8021-8027.
Chicago
Wang, J., Wang, S., Luan, Y., Zhang, W., Sun, C., Cheng, G., Li, K., Xin, Q., Lin, Z., Qi, T., Kong, F."Overexpression of NEDD9 in renal cell carcinoma is associated with tumor migration and invasion". Oncology Letters 14, no. 6 (2017): 8021-8027. https://doi.org/10.3892/ol.2017.7231
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Wang S, Luan Y, Zhang W, Sun C, Cheng G, Li K, Xin Q, Lin Z, Qi T, Qi T, et al: Overexpression of NEDD9 in renal cell carcinoma is associated with tumor migration and invasion. Oncol Lett 14: 8021-8027, 2017.
APA
Wang, J., Wang, S., Luan, Y., Zhang, W., Sun, C., Cheng, G. ... Kong, F. (2017). Overexpression of NEDD9 in renal cell carcinoma is associated with tumor migration and invasion. Oncology Letters, 14, 8021-8027. https://doi.org/10.3892/ol.2017.7231
MLA
Wang, J., Wang, S., Luan, Y., Zhang, W., Sun, C., Cheng, G., Li, K., Xin, Q., Lin, Z., Qi, T., Kong, F."Overexpression of NEDD9 in renal cell carcinoma is associated with tumor migration and invasion". Oncology Letters 14.6 (2017): 8021-8027.
Chicago
Wang, J., Wang, S., Luan, Y., Zhang, W., Sun, C., Cheng, G., Li, K., Xin, Q., Lin, Z., Qi, T., Kong, F."Overexpression of NEDD9 in renal cell carcinoma is associated with tumor migration and invasion". Oncology Letters 14, no. 6 (2017): 8021-8027. https://doi.org/10.3892/ol.2017.7231
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team