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Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis

  • Authors:
    • Feng Zhang
    • Deli Wang
    • Fasheng Chen
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, The Second People's Hospital of Liaocheng, Linqing, Shandong 252601, P.R. China, Department of Otorhinolaryngology, The Affiliated Hospital of Taishan Medical University, Tai'an, Shandong 271200, P.R. China, Department of Otolaryngology Head and Neck Surgery, Central Hospital of Enshi Autonomous Prefecture, Enshi, Hubei 445000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7829-7832
    |
    Published online on: October 20, 2017
       https://doi.org/10.3892/ol.2017.7235
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Abstract

The purpose of this study was to investigate the correlation between the expression of Toll-like receptor-9 (TLR-9) and cell proliferation and apoptosis in hypoxic nasopharyngeal carcinoma cells. Human nasopharyngeal carcinoma cell line HNE-1 (EBV positive) and CNE-1 (EBV negative) were used. Cells were divided into normal control group, hypoxia group and hyperoxia group. Hypoxic conditions were 5% CO2 and 0.01% partial pressure of oxygen, hyperoxia conditions were 5% CO2 and 10% partial pressure of oxygen. Reverse transcription-PCR (RT-PCR) and western blot analysis were used to detect the expression of TLR-9 mRNA and protein at 6, 12 and 24 h after the beginning of cell culture. MTT assay was used to detect the cell proliferation rate and flow cytometry was used to detect cell apoptosis rate. Expression levels of TLR-9 mRNA and protein in hypoxia group reached the peak at 12 h after the beginning of cell culture, and were significantly higher than those of hyperoxia group at all time‑points, expression levels of TLR-9 mRNA and protein of control group were the lowest, difference between groups were all statistically significant (P<0.05). No significant changes in expression levels of TLR-9 mRNA and protein were found in control group and hyperoxia group between different time-points (P>0.05). Compared with the other two groups, cell proliferation rate was gradually decreased and apoptotic rate was gradually decreased in hypoxia group, significant differences were found between hypoxia group, and control group and hyperoxia group (P<0.05), no significant differences were found between control group and hyperoxia group (P>0.05). In conclusion, TLR-9 was highly expressed in hypoxic nasopharyngeal carcinoma cells regulating cell proliferation and apoptosis, which may be an important mechanism of tumorigenesis and a potential target for intervention therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang F, Wang D and Chen F: Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis. Oncol Lett 14: 7829-7832, 2017.
APA
Zhang, F., Wang, D., & Chen, F. (2017). Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis. Oncology Letters, 14, 7829-7832. https://doi.org/10.3892/ol.2017.7235
MLA
Zhang, F., Wang, D., Chen, F."Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis". Oncology Letters 14.6 (2017): 7829-7832.
Chicago
Zhang, F., Wang, D., Chen, F."Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis". Oncology Letters 14, no. 6 (2017): 7829-7832. https://doi.org/10.3892/ol.2017.7235
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang F, Wang D and Chen F: Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis. Oncol Lett 14: 7829-7832, 2017.
APA
Zhang, F., Wang, D., & Chen, F. (2017). Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis. Oncology Letters, 14, 7829-7832. https://doi.org/10.3892/ol.2017.7235
MLA
Zhang, F., Wang, D., Chen, F."Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis". Oncology Letters 14.6 (2017): 7829-7832.
Chicago
Zhang, F., Wang, D., Chen, F."Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis". Oncology Letters 14, no. 6 (2017): 7829-7832. https://doi.org/10.3892/ol.2017.7235
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