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Article

Effects of HuR downregulation on anaplastic thyroid cancer cells

  • Authors:
    • Lorenzo Allegri
    • Catia Mio
    • Diego Russo
    • Sebastiano Filetti
    • Federica Baldan
  • View Affiliations / Copyright

    Affiliations: Department of Medical and Biological Sciences, University of Udine, I‑33100 Udine, Italy, Department of Health Sciences, ‘Magna Graecia’ University of Catanzaro, I‑88100 Catanzaro, Italy, Department of Internal Medicine and Medical Specialties, ‘Sapienza’ University of Rome, I‑00161 Rome, Italy
  • Pages: 575-579
    |
    Published online on: October 30, 2017
       https://doi.org/10.3892/ol.2017.7289
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Abstract

Anaplastic thyroid cancer (ATC) constitutes one of the most aggressive types of human solid cancer, and is characterized by the absence of thyroid differentiation features and a marked degree of invasiveness. We have previously demonstrated that the RNA‑binding protein Hu antigen R (HuR) is overexpressed in thyroid carcinoma; thus, the biological role of this RNA‑binding protein was investigated in the present study using the ATC cell lines SW1736 and 8505C. In both cell lines, HuR protein levels were higher compared with in the non‑tumorigenic thyroid cell line Nthy‑ori‑3.1. HuR silencing by RNA interference in both ATC cell lines decreased cell viability, increased apoptosis rates and reduced the capability to form colonies in soft agar. Thus, HuR plays an important role in the proliferation and aggressiveness of ATC cells. The histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) was able to reduce the viability of ATC cells. The results demonstrated that SAHA was able to decrease HuR expression in SW1736 and 8505C cells. Furthermore, since it is known that the transcription factor nuclear factor (NF)‑κB modulates HuR expression, whether SAHA affects the nuclear (active) fraction of NF‑κB in ATC cells was investigated. The data suggested that SAHA decreases ATC cell viability by reducing the active form of NF‑κB, which, in turn, modulates HuR expression.
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Copy and paste a formatted citation
Spandidos Publications style
Allegri L, Mio C, Russo D, Filetti S and Baldan F: Effects of HuR downregulation on anaplastic thyroid cancer cells. Oncol Lett 15: 575-579, 2018.
APA
Allegri, L., Mio, C., Russo, D., Filetti, S., & Baldan, F. (2018). Effects of HuR downregulation on anaplastic thyroid cancer cells. Oncology Letters, 15, 575-579. https://doi.org/10.3892/ol.2017.7289
MLA
Allegri, L., Mio, C., Russo, D., Filetti, S., Baldan, F."Effects of HuR downregulation on anaplastic thyroid cancer cells". Oncology Letters 15.1 (2018): 575-579.
Chicago
Allegri, L., Mio, C., Russo, D., Filetti, S., Baldan, F."Effects of HuR downregulation on anaplastic thyroid cancer cells". Oncology Letters 15, no. 1 (2018): 575-579. https://doi.org/10.3892/ol.2017.7289
Copy and paste a formatted citation
x
Spandidos Publications style
Allegri L, Mio C, Russo D, Filetti S and Baldan F: Effects of HuR downregulation on anaplastic thyroid cancer cells. Oncol Lett 15: 575-579, 2018.
APA
Allegri, L., Mio, C., Russo, D., Filetti, S., & Baldan, F. (2018). Effects of HuR downregulation on anaplastic thyroid cancer cells. Oncology Letters, 15, 575-579. https://doi.org/10.3892/ol.2017.7289
MLA
Allegri, L., Mio, C., Russo, D., Filetti, S., Baldan, F."Effects of HuR downregulation on anaplastic thyroid cancer cells". Oncology Letters 15.1 (2018): 575-579.
Chicago
Allegri, L., Mio, C., Russo, D., Filetti, S., Baldan, F."Effects of HuR downregulation on anaplastic thyroid cancer cells". Oncology Letters 15, no. 1 (2018): 575-579. https://doi.org/10.3892/ol.2017.7289
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