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Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro

  • Authors:
    • Pan Gao
    • Chunjie Li
    • Zheng Chang
    • Xiaoyi Wang
    • Ming Xuan
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 331-337
    |
    Published online on: October 31, 2017
       https://doi.org/10.3892/ol.2017.7301
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Abstract

Carcinoma-associated fibroblasts (CAFs) are dominant components of the tumor microenvironment (TME) that promote the development, progression and metastasis of cancer. c‑Met is a receptor of the hepatocyte growth factor (HGF), which is involved in lymphangiogenesis. Currently, the roles of CAFs during lymphangiogenesis are unknown. It has been hypothesized that CAFs contribute to lymphangiogenesis of oral squamous cell carcinoma (OSCC) via a HGF/c‑Met complex. The expression of HGF in OSCC was determined using CAFs derived from OSCC tissue and it was demonstrated that HGF is overexpressed in OSCC‑derived CAFs. It was also revealed that c‑Met was highly expressed in human lymphatic endothelial cells (HLECs) when co‑cultured with CAFs. Furthermore, it was demonstrated that recombinant human HGF significantly enhanced the proliferation, migration, invasion and tube formation of HLECs. By contrast, the inhibition of c‑Met expression suppressed the aforementioned biological activities and also downregulated the expression of c‑Met, phosphoinositide 3‑kinase and phosphorylated protein kinase B. Taken together, these data demonstrate that c‑Met is associated with the regulation of lymphangiogenesis. Thus, the results of the present study indicate that c‑Met may be a promising novel therapeutic target to treat patients with OSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Gao P, Li C, Chang Z, Wang X and Xuan M: Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro. Oncol Lett 15: 331-337, 2018.
APA
Gao, P., Li, C., Chang, Z., Wang, X., & Xuan, M. (2018). Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro. Oncology Letters, 15, 331-337. https://doi.org/10.3892/ol.2017.7301
MLA
Gao, P., Li, C., Chang, Z., Wang, X., Xuan, M."Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro". Oncology Letters 15.1 (2018): 331-337.
Chicago
Gao, P., Li, C., Chang, Z., Wang, X., Xuan, M."Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro". Oncology Letters 15, no. 1 (2018): 331-337. https://doi.org/10.3892/ol.2017.7301
Copy and paste a formatted citation
x
Spandidos Publications style
Gao P, Li C, Chang Z, Wang X and Xuan M: Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro. Oncol Lett 15: 331-337, 2018.
APA
Gao, P., Li, C., Chang, Z., Wang, X., & Xuan, M. (2018). Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro. Oncology Letters, 15, 331-337. https://doi.org/10.3892/ol.2017.7301
MLA
Gao, P., Li, C., Chang, Z., Wang, X., Xuan, M."Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro". Oncology Letters 15.1 (2018): 331-337.
Chicago
Gao, P., Li, C., Chang, Z., Wang, X., Xuan, M."Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro". Oncology Letters 15, no. 1 (2018): 331-337. https://doi.org/10.3892/ol.2017.7301
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