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Article Open Access

Effect of Ku80 on the radiosensitization of cisplatin in the cervical carcinoma cell line HeLa

  • Authors:
    • Liang Zhuang
    • Fei Liu
    • Ping Peng
    • Huihua Xiong
    • Hong Qiu
    • Xiugen Fu
    • Zhiping Xiao
    • Xiaoyuan Huang
  • View Affiliations / Copyright

    Affiliations: Cancer Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
    Copyright: © Zhuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 147-154
    |
    Published online on: October 31, 2017
       https://doi.org/10.3892/ol.2017.7304
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Abstract

Cisplatin chemotherapy in combination with radiotherapy is the primary therapeutic strategy for the treatment of cervical cancer; however, the underlying molecular mechanism for cisplatin radiosensitization remains unknown. The aim of the present study was to investigate the effect of Ku80, a DNA double‑strand break (DSB) repair protein, on cisplatin radiosensitization in cervical cancer. The pre‑established Ku80 suppression cervical cancer cell line HeLa/Ku80‑siRNA and the normal HeLa cell line underwent 6 MV X‑ray irradiation (6 Gy) individually or in combination with 5 µg/ml cisplatin treatment. Alterations in apoptosis, the cell cycle and γH2AX expression were detected. Following irradiation individually and combined with cisplatin, compared with normal HeLa cells, HeLa/Ku80-siRNAexhibited an increased rate of apoptosis (P<0.05). It was identified that the earlier cisplatin was administered following irradiation, the higher the rate of apoptosis. Cell cycle analysis indicated that, following irradiation combined with cisplatin, the cells were arrested in G1 and S phase rather than in G2/M phase following irradiation alone. Microscopic imaging of immunofluorescence staining and western blotting identified that HeLa/Ku80‑siRNA cells exhibited more γH2AX foci remaining following treatment with irradiation and cisplatin, particularly in the group treated with 6 Gy irradiation for 1 h together with 23 h of exposure to cisplatin. Irradiation in combination with cisplatin promoted the apoptosis of HeLa cells in association with the inhibition of Ku80, and it was identified that the earlier cisplatin was administered following irradiation, the more apoptosis was induced. This maybe because irradiation combined with cisplatin is able to arrest cells in G1 and S phase to rapidly repair damaged DNA, and the lack of Ku80 induces the inability to repair DSB, resulting in increased apoptosis. The results of the present study suggest that Ku80 may be a potent molecular target in cisplatin radiosensitization.
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Copy and paste a formatted citation
Spandidos Publications style
Zhuang L, Liu F, Peng P, Xiong H, Qiu H, Fu X, Xiao Z and Huang X: Effect of Ku80 on the radiosensitization of cisplatin in the cervical carcinoma cell line HeLa. Oncol Lett 15: 147-154, 2018.
APA
Zhuang, L., Liu, F., Peng, P., Xiong, H., Qiu, H., Fu, X. ... Huang, X. (2018). Effect of Ku80 on the radiosensitization of cisplatin in the cervical carcinoma cell line HeLa. Oncology Letters, 15, 147-154. https://doi.org/10.3892/ol.2017.7304
MLA
Zhuang, L., Liu, F., Peng, P., Xiong, H., Qiu, H., Fu, X., Xiao, Z., Huang, X."Effect of Ku80 on the radiosensitization of cisplatin in the cervical carcinoma cell line HeLa". Oncology Letters 15.1 (2018): 147-154.
Chicago
Zhuang, L., Liu, F., Peng, P., Xiong, H., Qiu, H., Fu, X., Xiao, Z., Huang, X."Effect of Ku80 on the radiosensitization of cisplatin in the cervical carcinoma cell line HeLa". Oncology Letters 15, no. 1 (2018): 147-154. https://doi.org/10.3892/ol.2017.7304
Copy and paste a formatted citation
x
Spandidos Publications style
Zhuang L, Liu F, Peng P, Xiong H, Qiu H, Fu X, Xiao Z and Huang X: Effect of Ku80 on the radiosensitization of cisplatin in the cervical carcinoma cell line HeLa. Oncol Lett 15: 147-154, 2018.
APA
Zhuang, L., Liu, F., Peng, P., Xiong, H., Qiu, H., Fu, X. ... Huang, X. (2018). Effect of Ku80 on the radiosensitization of cisplatin in the cervical carcinoma cell line HeLa. Oncology Letters, 15, 147-154. https://doi.org/10.3892/ol.2017.7304
MLA
Zhuang, L., Liu, F., Peng, P., Xiong, H., Qiu, H., Fu, X., Xiao, Z., Huang, X."Effect of Ku80 on the radiosensitization of cisplatin in the cervical carcinoma cell line HeLa". Oncology Letters 15.1 (2018): 147-154.
Chicago
Zhuang, L., Liu, F., Peng, P., Xiong, H., Qiu, H., Fu, X., Xiao, Z., Huang, X."Effect of Ku80 on the radiosensitization of cisplatin in the cervical carcinoma cell line HeLa". Oncology Letters 15, no. 1 (2018): 147-154. https://doi.org/10.3892/ol.2017.7304
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