Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
January-2018 Volume 15 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2018 Volume 15 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model

  • Authors:
    • Hongmei Xia
    • Xiaojing Luo
    • Weihua Yin
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The People's Hospital of Yichun Affiliated to Clinical Medicine School of Yichun University in Jiangxi Province, Yichun, Jiangxi 336000, P.R. China
  • Pages: 538-544
    |
    Published online on: November 2, 2017
       https://doi.org/10.3892/ol.2017.7332
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Advanced prostate cancer is difficult to treat owing to a lack of effective approaches for disrupting immune tolerance. C57BL/6 male and female mice implanted with viable RM‑1 cells represent a novel murine model of advanced prostate cancer for studying antitumor effects following immunization with a granulocyte‑macrophage colony‑stimulating factor (GM‑CSF)‑modified RM‑1 cell vaccine, which has been described previously. In vitro cytotoxic activity and cytokine secretion experiments were conducted to investigate the antitumor response. The cytotoxicity profile of splenocytes from female mice immunized against RM‑1 cells primarily involved cytotoxic T lymphocyte (CTL) lysis and, to a lesser extent, natural killer (NK) cell lysis. NK cell lysis was also observed in males, which exhibited no evidence of CTL lysis. The secretion of interferon‑γ in the GM‑CSF‑modified cell vaccine group was significantly increased compared with the other groups. The level of interleukin‑4 was low. To investigate the antitumor immune response further, cluster of differentiation 4 (CD4) T cells and CD8 T cells were analyzed in the spleens and tumors of female mice receiving the GM‑CSF‑modified RM‑1 cell vaccine. Unlike female mice, males exhibited the highest proportion of NK cells in the spleen. NK cells were not detected in the tumor tissue in any of the groups. The difference between the sexes may explain the specificity of the immune response, as females are intolerant to prostate antigens whereas males are. This model is clinically relevant as it translates to human immunology and offers an effective and convenient method for studying immunotherapy for prostate cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Crawford ED, Blumenstein BA, Goodman PJ, Davis MA, Eisenberger MA, McLeod DG, Spaulding JT, Benson R and Dorr FA: Leuprolide with and without flutamide in advanced prostate cancer. Cancer. 66(5 Suppl): S1039–S1044. 1990. View Article : Google Scholar

3 

Quinn DI, Tangen CM, Hussain M, Lara PN Jr, Goldkorn A, Moinpour CM, Garzotto MG, Mack PC, Carducci MA, Monk JP, et al: Docetaxel and atrasentan versus docetaxel and placebo for men with advanced castration-resistant prostate cancer (SWOG S0421): A randomised phase 3 trial. Lancet Oncol. 14:893–900. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Armstrong AJ and Carducci MA: Chemotherapy for advanced prostate cancer: Results of new clinical trials and future studies. Curr Oncol Rep. 7:220–227. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Haigh PI, Difronzo LA, Gammon G and Morton DL: Vaccine therapy for patients with melanoma. Oncology (Williston Park). 13:1561–1574. 1999.PubMed/NCBI

6 

Li H, Jiang HJ, Ma MQ, Wei F, An XM and Ren XB: Vaccination with allogeneic GM-CSF gene-modified lung cancer cells: Antitumor activity comparing with that induced by autologous vaccine. Cancer Biother Radiopharm. 22:790–798. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Obata C, Zhang M, Moroi Y, Hisaeda H, Tanaka K, Murata S, Furue M and Himeno K: Formalin-fixed tumor cells effectively induce antitumor immunity both in prophylactic and therapeutic conditions. J Dermatol Sci. 34:209–219. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Yin W, He Q, Hu Z, Chen Z, Qifeng M, Zhichun S, Zhihui Q, Xiaoxia N, Li J and Gao J: A novel therapeutic vaccine of GM-CSF/TNFalpha surface-modified RM-1 cells against the orthotopic prostatic cancer. Vaccine. 28:4937–4944. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Suckow MA, Wolter WR and Pollard M: Prevention of de novo prostate cancer by immunization with tumor-derived vaccines. Cancer Immunol Immunother. 54:571–576. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Kotera Y, Shimizu K and Mulé JJ: Comparative analysis of necrotic and apoptotic tumor cells as a source of antigen(s) in dendritic cell-based immunization. Cancer Res. 61:8105–8109. 2001.PubMed/NCBI

11 

Ahram M, Flaig MJ, Gillespie JW, Duray PH, Linehan WM, Ornstein DK, Niu S, Zhao Y, Petricoin EF III and Emmert-Buck MR: Evaluation of ethanol-fixed, paraffin-embedded tissues for proteomic applications. Proteomics. 3:413–421. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Baley PA, Yoshida K, Qian W, Sehgal I and Thompson TC: Progression to androgen insensitivity in a novel in vitro mouse model for prostate cancer. J Steroid Biochem Mol Biol. 52:403–413. 1995. View Article : Google Scholar : PubMed/NCBI

13 

Griffith TS, Kawakita M, Tian J, Ritchey J, Tartaglia J, Sehgal I, Thompson TC, Zhao W and Ratliff TL: Inhibition of murine prostate tumor growth and activation of immunoregulatory cells with recombinant canarypox viruses. J Natl Cancer Inst. 93:998–1007. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Labarthe MC, Theocharous P, Russell N, Todryk S, Bangma C, Thraves P, Dalgleish AG and Whelan MA: A novel murine model of allogeneic vaccination against prostate cancer. Cancer Immunol Immunother. 57:453–465. 2008. View Article : Google Scholar : PubMed/NCBI

15 

He Q, Li J, Yin W, Song Z, Zhang Z, Yi T, Tang J, Wu D, Lu Y, Wang Z, et al: Low-dose paclitaxel enhances the anti-tumor efficacy of GM-CSF surface-modified whole-tumor-cell vaccine in mouse model of prostate cancer. Cancer Immunol Immunother. 60:715–730. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Cooper MD and Alder MN: The evolution of adaptive immune systems. Cell. 124:815–822. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Li M, Davey GM, Sutherland RM, Kurts C, Lew AM, Hirst C, Carbone FR and Heath WR: Cell-associated ovalbumin is cross-presented much more efficiently than soluble ovalbumin in vivo. J Immunol. 166:6099–6103. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Gallucci S, Lolkema M and Matzinger P: Natural adjuvants: Endogenous activators of dendritic cells. Nat Med. 5:1249–1255. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Sauter B, Albert ML, Francisco L, Larsson M, Somersan S and Bhardwaj N: Consequences of cell death: Exposure to necrotic tumor cells, but not primary tissue cells or apoptotic cells, induces the maturation of immunostimulatory dendritic cells. J Exp Med. 191:423–434. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Grant JF, Iwasawa T, Sinn HW, Siemens DR, Griffith TS, Takacs EB and Ratliff TL: Induction of protective immunity to RM-1 prostate cancer cells with ALVAC-IL-2/IL-12/TNF-alpha combination therapy. Int J Cancer. 119:2632–2641. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Carbone FR, Kurts C, Bennett SR, Miller JF and Heath WR: Cross-presentation: A general mechanism for CTL immunity and tolerance. Immunol Today. 19:368–373. 1998. View Article : Google Scholar : PubMed/NCBI

22 

Chhikara M, Huang H, Vlachaki MT, Zhu X, Teh B, Chiu KJ, Woo S, Berner B, Smith EO, Oberg KC, et al: Enhanced therapeutic effect of HSV-tk+GCV gene therapy and ionizing radiation for prostate cancer. Mol Ther. 3:536–542. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Schroten-Loef C, de Ridder CM, Reneman S, Crezee M, Dalgleish A, Todryk SM, Bangma CH and Kraaij R: A prostate cancer vaccine comprising whole cells secreting IL-7, effective against subcutaneous challenge, requires local GM-CSF for intra-prostatic efficacy. Cancer Immunol Immunother. 58:373–381. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xia H, Luo X and Yin W: Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model. Oncol Lett 15: 538-544, 2018.
APA
Xia, H., Luo, X., & Yin, W. (2018). Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model. Oncology Letters, 15, 538-544. https://doi.org/10.3892/ol.2017.7332
MLA
Xia, H., Luo, X., Yin, W."Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model". Oncology Letters 15.1 (2018): 538-544.
Chicago
Xia, H., Luo, X., Yin, W."Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model". Oncology Letters 15, no. 1 (2018): 538-544. https://doi.org/10.3892/ol.2017.7332
Copy and paste a formatted citation
x
Spandidos Publications style
Xia H, Luo X and Yin W: Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model. Oncol Lett 15: 538-544, 2018.
APA
Xia, H., Luo, X., & Yin, W. (2018). Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model. Oncology Letters, 15, 538-544. https://doi.org/10.3892/ol.2017.7332
MLA
Xia, H., Luo, X., Yin, W."Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model". Oncology Letters 15.1 (2018): 538-544.
Chicago
Xia, H., Luo, X., Yin, W."Inhibition of prostate cancer growth by immunization with a GM-CSF-modified mouse prostate cancer RM‑1 cell vaccine in a novel murine model". Oncology Letters 15, no. 1 (2018): 538-544. https://doi.org/10.3892/ol.2017.7332
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team