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Article

Identification of proteins with the CDw75 epitope in human colorectal cancer

  • Authors:
    • Óscar Mariño‑Crespo
    • Almudena Fernández‑Briera
    • Emilio Gil‑Martín
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Genetics and Immunology, Biomedical Research Center (CINBIO, ‘Centro Singular de Investigación de Galicia’), University of Vigo, 36310 Vigo, Spain
  • Pages: 580-587
    |
    Published online on: November 2, 2017
       https://doi.org/10.3892/ol.2017.7336
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Abstract

The CDw75 epitope is an α(2,6) sialylated antigen overexpressed in colorectal cancer (CRC), where its expression correlates with the progression of the disease. The CDw75 epitope is located mainly in N‑glycoproteins, whose identity remains unknown. The aim of the present study was to identify proteins with the CDw75 epitope as a strategy to deepen the understanding of molecular pathogenesis of CRC and to identify novel biomarkers for this disease. For this purpose, a two‑dimensional electrophoresis approach was employed. Protein spots in the gels were matched to the corresponding CDw75 positive spots in the immunoblotted polyvinylidene difluoride membranes, and further identification of the protein species was performed by mass spectrometry. Additionally, one‑dimensional western blotting experiments were performed to verify the expression of these candidate proteins in the colorectal tissue and their coincidence in molecular mass with the CDw75‑positive bands. The findings of the present study indicate that haptoglobin and the keratins 8 (K8) and 18 (K18) are proteins with the CDw75 epitope in the colorectal tissue from CRC patients and also suggest novel functions and cellular locations for these proteins in the colorectal tissue and in relation to CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Mariño‑Crespo Ó, Fernández‑Briera A and Gil‑Martín E: Identification of proteins with the CDw75 epitope in human colorectal cancer. Oncol Lett 15: 580-587, 2018.
APA
Mariño‑Crespo, Ó., Fernández‑Briera, A., & Gil‑Martín, E. (2018). Identification of proteins with the CDw75 epitope in human colorectal cancer. Oncology Letters, 15, 580-587. https://doi.org/10.3892/ol.2017.7336
MLA
Mariño‑Crespo, Ó., Fernández‑Briera, A., Gil‑Martín, E."Identification of proteins with the CDw75 epitope in human colorectal cancer". Oncology Letters 15.1 (2018): 580-587.
Chicago
Mariño‑Crespo, Ó., Fernández‑Briera, A., Gil‑Martín, E."Identification of proteins with the CDw75 epitope in human colorectal cancer". Oncology Letters 15, no. 1 (2018): 580-587. https://doi.org/10.3892/ol.2017.7336
Copy and paste a formatted citation
x
Spandidos Publications style
Mariño‑Crespo Ó, Fernández‑Briera A and Gil‑Martín E: Identification of proteins with the CDw75 epitope in human colorectal cancer. Oncol Lett 15: 580-587, 2018.
APA
Mariño‑Crespo, Ó., Fernández‑Briera, A., & Gil‑Martín, E. (2018). Identification of proteins with the CDw75 epitope in human colorectal cancer. Oncology Letters, 15, 580-587. https://doi.org/10.3892/ol.2017.7336
MLA
Mariño‑Crespo, Ó., Fernández‑Briera, A., Gil‑Martín, E."Identification of proteins with the CDw75 epitope in human colorectal cancer". Oncology Letters 15.1 (2018): 580-587.
Chicago
Mariño‑Crespo, Ó., Fernández‑Briera, A., Gil‑Martín, E."Identification of proteins with the CDw75 epitope in human colorectal cancer". Oncology Letters 15, no. 1 (2018): 580-587. https://doi.org/10.3892/ol.2017.7336
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