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Article

Significant expression of CHK1 and p53 in bladder urothelial carcinoma as potential therapeutic targets and prognosis

  • Authors:
    • Linfeng Zheng
    • Yuping Zhu
    • Lei Lei
    • Wenyong Sun
    • Guoping Cheng
    • Shifeng Yang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China, Department of Colorectal Surgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China, Department of Medical Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China
  • Pages: 568-574
    |
    Published online on: November 3, 2017
       https://doi.org/10.3892/ol.2017.7344
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Abstract

Checkpoint kinase 1 (CHK1) and p53 are involved in cell‑cycle checkpoint, and cellular response to DNA damage. CHK1 and p53 are overexpressed in bladder urothelial carcinoma (BUC); however, a clear elucidation on their interaction and influence in the progress of BUC is absent. The aim of the present study was to examine the correlation between CHK1 and p53 in BUC, and analyze their value as therapeutic targets and prognostic indicators in BUC. A clinically annotated cohort of 110 patients with BUC was identified retrospectively. EnVision‑based immunohistochemistry and western blot analysis of the aforementioned DNA repair proteins were conducted on formalin‑fixed‑paraffin‑embedded or frozen tissues from the primary tumor. A total of 45 peritumoral tissue cases were assessed similarly as the control group. In the cohort of 110 patients with BUC, a significant overexpression of CHK1 and p53 was observed in primary compared with the peritumoral tissues (P<0.05). CHK1 and p53 demonstrated a positive correlation in BUC, and both were positively associated with the histological grade, clinical pathological staging, lymphatic metastasis and the 5‑year survival rate (P<0.05). However, CHK1 and p53 were not associated with sex, age, tumor diameter, single/multiple sites or incipient/recurrence. The overexpression of CHK1 and p53, and their synergistic interaction were putatively correlated with the physiology of BUC that may be deemed as potential therapeutic targets and prognostic indicators.
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1 

Miller KD, Siegel RL, Lin CC, Mariotto AB, Kramer JL, Rowland JH, Stein KD, Alteri R and Jemal A: Cancer treatment and survivorship statistics, 2016. CA Cancer J Clin. 66:271–289. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Prasad SM, Decastro GJ and Steinberg GD: Medscape: URothelial carcinoma of the bladder: Definition, treatment and future efforts. Nat Rev Urol. 8:631–642. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Abdollah F, Gandaglia G, Thuret R, Schmitges J, Tian Z, Jeldres C, Passoni NM, Briganti A, Shariat SF, Perrotte P, et al: Incidence, survival and mortality rates of stage-specific bladder cancer in united states: A trend analysis. Cancer Epidemiol. 37:219–225. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Bishr M, Lattouf JB, Latour M and Saad F: Tumour stage on re-staging transurethral resection predicts recurrence anad progression-free survival of patients with high-risk non-muscle invasive bladder cancer. Can Urol Assoc J. 8:E306–E310. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Yafi FA, Aprikian AG, Chin JL, Fradet Y, Izawa J, Estey E, Fairey A, Rendon R, Cagiannos I, Lacombe L, et al: Contemporary outcomes of 2287 patients with bladder cancer who were treated with radical cystectomy: A canadian multicentre experience. BJU Int. 108:539–545. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Soloway MS: Bladder cancer: Lack of progress in bladder cancer-what are the obstacles? Nat Rev Urol. 10:5–6. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Nedjadi T, Al-Maghrabi J, Assidi M, Dallol A, Al-Kattabi H, Chaudhary A, Al-Sayyad A, Al-Ammari A, Abuzenadah A, Buhmeida A and Al-Qahtani M: Prognostic value of HER2 status in bladder transitional cell carcinoma revealed by both IHC and BDISH techniques. BMC Cancer. 16:6532016. View Article : Google Scholar : PubMed/NCBI

8 

Wang ZY, Zhang W, Yang JJ, Song DK, Wei JX and Gao S: Association of thymosin β4 expression with clinicopathological parameters and clinical outcomes of bladder cancer patients. Neoplasma. 63:991–998. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Zhou J, Dong D, Cheng R, Wang Y, Jiang S, Zhu Y, Fan L, Mao X, Gui Y, Li Z, et al: Aberrant expression of KPNA2 is associated with a poor prognosis and contributes to OCT4 nuclear transportation in bladder cancer. Oncotarget. 7:72767–72776. 2016.PubMed/NCBI

10 

van der Heijden AG, Mengual L, Lozano JJ, Ingelmo-Torres M, Ribal MJ, Fernandez PL, Oosterwijk E, Schalken JA, Alcaraz JA, Alcaraz A and Witjes JA: A five-gene expression signature to predict progression in T1G3 bladder cancer. Eur J Cancer. 64:127–136. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Pignot G, Cizeron-Clairac G, Vacher S, Susini A, Tozlu S, Vieillefond A, Zerbib M, Lidereau R, Debre B, Amsellem-Ouazana D and Bieche I: microRNA expression profile in a large series of bladder tumors: identification of a 3-miRNA signature associated with aggressiveness of muscle-invasive bladder cancer. Int J Cancer. 132:2479–2491. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Segersten U, Spector Y, Goren Y, Tabak S and Malmström PU: The role of microRNA profiling in prognosticating progression in Ta and T1 urinary bladder cancer. Urol Oncol. 32:613–618. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Goto H, Kasahara K and Inagaki M: Novel insights into Chk1 regulation by phosphorylation. Cell Struct Funct. 40:43–50. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Toledo LI, Murga M and Fernandez-Capetillo O: Targeting ATR and Chk1 kinases for cancer treatment: A new model for new (and old) drugs. Mol Oncol. 5:368–373. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Petermann E, Maya-Mendoza A, Zachos G, Gillespie DA, Jackson DA and Caldecott KW: Chk1 requirement for high global rates of replication fork progression during normal vertebrate S phase. Mol Cell Biol. 26:3319–3326. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Petermann E, Woodcock M and Helleday T: Chk1 promotes replication fork progression by controlling replication initiation. Proc Natl Acad Sci USA. 107:pp. 16090–16095. 2010; View Article : Google Scholar : PubMed/NCBI

17 

Herman-Antosiewicz A, Stan SD, Hahm ER, Xiao D and Singh SV: Activation of a novel ataxia-telangiectasia mutated and Rad3 related/checkpoint kinase 1-dependent prometaphase checkpoint in cancer cells by diallyl trisulfide, a promising cancer chemopreventive constituent of processed garlic. Mol Cancer Ther. 6:1249–1261. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Myers K, Gagou ME, Zuazua-Villar P, Rodriguez R and Meuth M: ATR and Chk1 suppress a caspase-3-dependent apoptotic response following DNA replication stress. PLoS Genet. 5:e10003242009. View Article : Google Scholar : PubMed/NCBI

19 

Wasylishen AR and Lozano G: Attenuating the p53 pathway in human cancers: many means to the same end. Cold Spring Harb Perspect Med. 6:a0262112016. View Article : Google Scholar : PubMed/NCBI

20 

Vazquez A, Bond EE, Levine AJ and Bond GL: The genetics of the p53 pathway, apoptosis and cancer therapy. Nat Rev Drug Discov. 7:979–987. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Williams AB and Schumacher B: p53 in the DNA-Damage-Repair Process. Cold Spring Harb Perspect Med. 6:2016. View Article : Google Scholar : PubMed/NCBI

22 

Freed-Pastor WA and Prives C: Mutant p53: One name, many proteins. Genes Dev. 26:1268–1286. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Humphrey PA, Moch H, Cubilla AL, Ulbright TM and Reuter VE: The 2016 WHO classification of tumours of the urinary system and male genital organs-part B: Prostate and bladder tumours. Eur Urol. 70:106–119. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Rath S, Connors JM, Dolman PJ, Rootman J, Rootman DB and White VA: Comparison of American Joint Committee on Cancer TNM-based staging system (7th edition) and Ann Arbor Classification for predicting outcome in ocular adnexal lymphoma. Orbit. 33:23–28. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Drayton RM and Catto JW: Molecular mechanisms of cisplatin resistance in bladder cancer. Expert Rev Anticancer Ther. 12:271–281. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Li CC, Yang JC, Lu MC, Lee CL, Peng CY, Hsu WY, Dai YH, Chang FR, Zhang DY, Wu WJ and Wu YC: ATR-Chk1 signaling inhibition as a therapeutic strategy to enhance cisplatin chemosensitivity in urothelial bladder cancer. Oncotarget. 7:1947–1959. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Umezawa Y, Kurosu T, Akiyama H, Wu N, Nogami A, Nagao T and Miura O: Down regulation of Chk1 by p53 plays a role in synergistic induction of apoptosis by chemotherapeutics and inhibitors for Jak2 or BCR/ABL in hematopoietic cells. Oncotarget. 7:44448–44461. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Manic G, Obrist F, Sistigu A and Vitale I: Trial Watch: Targeting ATM-CHK2 and ATR-CHK1 pathways for anticancer therapy. Mol Cell Oncol. 2:e10129762015. View Article : Google Scholar : PubMed/NCBI

29 

Bernard JJ, Lou YR, Peng QY, Li T, Conney AH and Lu YP: Inverse relationship between p53 and phospho-Chk1 (Ser317) protein expression in UVB-induced skin tumors in SKH-1 mice. Exp Mol Pathol. 96:126–131. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zheng L, Zhu Y, Lei L, Sun W, Cheng G and Yang S: Significant expression of CHK1 and p53 in bladder urothelial carcinoma as potential therapeutic targets and prognosis. Oncol Lett 15: 568-574, 2018.
APA
Zheng, L., Zhu, Y., Lei, L., Sun, W., Cheng, G., & Yang, S. (2018). Significant expression of CHK1 and p53 in bladder urothelial carcinoma as potential therapeutic targets and prognosis. Oncology Letters, 15, 568-574. https://doi.org/10.3892/ol.2017.7344
MLA
Zheng, L., Zhu, Y., Lei, L., Sun, W., Cheng, G., Yang, S."Significant expression of CHK1 and p53 in bladder urothelial carcinoma as potential therapeutic targets and prognosis". Oncology Letters 15.1 (2018): 568-574.
Chicago
Zheng, L., Zhu, Y., Lei, L., Sun, W., Cheng, G., Yang, S."Significant expression of CHK1 and p53 in bladder urothelial carcinoma as potential therapeutic targets and prognosis". Oncology Letters 15, no. 1 (2018): 568-574. https://doi.org/10.3892/ol.2017.7344
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng L, Zhu Y, Lei L, Sun W, Cheng G and Yang S: Significant expression of CHK1 and p53 in bladder urothelial carcinoma as potential therapeutic targets and prognosis. Oncol Lett 15: 568-574, 2018.
APA
Zheng, L., Zhu, Y., Lei, L., Sun, W., Cheng, G., & Yang, S. (2018). Significant expression of CHK1 and p53 in bladder urothelial carcinoma as potential therapeutic targets and prognosis. Oncology Letters, 15, 568-574. https://doi.org/10.3892/ol.2017.7344
MLA
Zheng, L., Zhu, Y., Lei, L., Sun, W., Cheng, G., Yang, S."Significant expression of CHK1 and p53 in bladder urothelial carcinoma as potential therapeutic targets and prognosis". Oncology Letters 15.1 (2018): 568-574.
Chicago
Zheng, L., Zhu, Y., Lei, L., Sun, W., Cheng, G., Yang, S."Significant expression of CHK1 and p53 in bladder urothelial carcinoma as potential therapeutic targets and prognosis". Oncology Letters 15, no. 1 (2018): 568-574. https://doi.org/10.3892/ol.2017.7344
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