Effects of Grb2‑associated binding protein 2‑specific siRNA on the migration and invasion of MG‑63 osteosarcoma cells

Wang,Huan; He,Hui; Meng,Hongmei; Cui,Yang; Wang,Wenbo

doi:10.3892/ol.2017.7375

Effects of Grb2‑associated binding protein 2‑specific siRNA on the migration and invasion of MG‑63 osteosarcoma cells

Authors:
- Huan Wang
- Hui He
- Hongmei Meng
- Yang Cui
- Wenbo Wang
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Affiliations: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Orthopedic Surgery, The First Hospital of Qiqihar, Qiqihar, Heilongjiang 161000, P.R. China
Published online on: November 9, 2017 https://doi.org/10.3892/ol.2017.7375
Pages: 926-930
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To investigate the association between the expression of growth factor receptor binding protein 2‑associated binding protein 2 (Gab2) in human osteosarcoma as well as the effects of Gab2 on invasion and metastasis, human MG‑63 osteosarcoma cells were transfected with small interfering (si)RNA plasmid. Gab2 protein and mRNA expression levels were detected using western blotting and reverse transcription‑polymerase chain reaction, respectively. The cell migration and invasion abilities were detected using in vitro chemotaxis and invasion assays, respectively, following siRNA vector expression. Gab2 was markedly expressed in MG‑63 cells. The Gab2 protein and mRNA expression levels of the cells transfected with Gab2 siRNA (siGab2/MG‑63) were reduced compared with those of the cells transfected with scrambled siRNA (Scr/MG‑63). The chemotaxis assay demonstrated that the migration capacity of siGab2/MG‑63 cells induced by 10 µg/l epidermal growth factor, was significantly reduced compared with that of the MG‑63 and Scr/MG‑63 cells (P<0.01). In comparison with Scr/MG‑63 and MG‑63 cells, a reduced number of siGab2/MG‑63 cells invaded the Matrigel matrix, demonstrating that the in vitro invasion capacity was significantly decreased (P<0.01). Decreasing Gab2 expression levels using siRNA interference inhibited the migration and invasion ability of human MG‑63 osteosarcoma cells.

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