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MicroRNA-27a functions as an oncogene in human osteosarcoma by targeting CCNG1

  • Authors:
    • Tao Lin
    • Quanping Ma
    • Yuefeng Zhang
    • Hongfei Zhang
    • Jiapeng Yan
    • Changhong Gao
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250000, P.R. China, Clinical Laboratory, Jinan Fourth People's Hospital, Jinan, Shandong 250000, P.R. China, Major Surgery, Shandong Yellow River Hospital, Jinan, Shandong 250000, P.R. China, Department of Orthopedics, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1067-1071
    |
    Published online on: November 10, 2017
       https://doi.org/10.3892/ol.2017.7389
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Abstract

Osteosarcoma is the most common type of malignant tumor arising from bone in children and adolescents. Accumulating evidences have shown the aberrant expression of numerous miRNAs is associated with the development and metastasis of osteosarcoma. The present study was conducted to investigate miR-27a expression in osteosarcoma tissues and cells. In the present study, quantitative RT-qPCR was used to measure the expression levels of miRNA and mRNA in osteosarcoma tissues and cells. Transwell assays were used to detect the effects of miR-27a on the invasive and migratory potential of cells. Luciferase reporter and western blot analysis were conducted to confirm cyclin G1 (CCNG1) as the target gene of miR-27a. The results showed that miR-27a was significantly upregulated in human osteosarcoma tissues and cell lines. The western blot analysis revealed that the overexpression of miR-27a suppressed CCNG1 protein expression. Luciferase reporter assays confirmed that CCNG1 is a direct target of miR-27a in osteosarcoma cells. The results suggest that miR-27a downregulates CCNG1 expression in osteosarcoma and acts as an oncogene directly targeting CCNG1. Thus, the miR-27a/CCNGI axis is a potential therapeutic target for human osteosarcoma.
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1 

Geller DS and Gorlick R: Osteosarcoma: A review of diagnosis, management, and treatment strategies. Clin Adv Hematol Oncol. 8:705–718. 2010.PubMed/NCBI

2 

Raymond AK and Jaffe N: Osteosarcoma multidisciplinary approach to the management from the pathologist's perspective. Cancer Treat Res. 152:63–84. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Ferrari S, Mercuri M, Bacci G, Bielack SS and Jürgens H: Comment on ‘Prognostic factors in high-grade osteosarcoma of the extremities or trunk: An analysis of 1,702 patients treated on neoadjuvant Cooperative Osteosarcoma Study Group protocols’. J Clin Oncol. 20:2910–2911. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Broadhead ML, Clark JC, Myers DE, Dass CR and Choong PF: The molecular pathogenesis of osteosarcoma: A review. Sarcoma. 2011:9592482011. View Article : Google Scholar : PubMed/NCBI

5 

He JP, Hao Y, Wang XL, Yang XJ, Shao JF, Guo FJ and Feng JX: Review of the molecular pathogenesis of osteosarcoma. Asian Pac J Cancer Prev. 15:5967–5976. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Fang Z, Du R, Edwards A, Flemington EK and Zhang K: The sequence structures of human microRNA molecules and their implications. PLoS One. 8:e542152013. View Article : Google Scholar : PubMed/NCBI

7 

He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Nicoloso MS, Spizzo R, Shimizu M, Rossi S and Calin GA: MicroRNAs - the micro steering wheel of tumour metastases. Nat Rev Cancer. 9:293–302. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Bueno MJ, Pérez de Castro I and Malumbres M: Control of cell proliferation pathways by microRNAs. Cell Cycle. 7:3143–3148. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Saumet A and Lecellier CH: microRNAs and personalized medicine: Evaluating their potential as cancer biomarkers. Adv Exp Med Biol. 888:5–15. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Nugent M: microRNA and bone cancer. Adv Exp Med Biol. 889:201–230. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Jones KB, Salah Z, Del Mare S, Galasso M, Gaudio E, Nuovo GJ, Lovat F, LeBlanc K, Palatini J, Randall RL, et al: miRNA signatures associate with pathogenesis and progression of osteosarcoma. Cancer Res. 72:1865–1877. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Gougelet A, Pissaloux D, Besse A, Perez J, Duc A, Dutour A, Blay JY and Alberti L: Micro-RNA profiles in osteosarcoma as a predictive tool for ifosfamide response. Int J Cancer. 129:680–690. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Pan W, Wang H, Jianwei R and Ye Z: MicroRNA-27a promotes proliferation, migration and invasion by targeting MAP2K4 in human osteosarcoma cells. Cell Physiol Biochem. 33:402–412. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Salah Z, Arafeh R, Maximov V, Galasso M, Khawaled S, Abou-Sharieha S, Volinia S, Jones KB, Croce CM and Aqeilan RI: miR-27a and miR-27a* contribute to metastatic properties of osteosarcoma cells. Oncotarget. 6:4920–4935. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Tamura K, Kanaoka Y, Jinno S, Nagata A, Ogiso Y, Shimizu K, Hayakawa T, Nojima H and Okayama H: Cyclin G: A new mammalian cyclin with homology to fission yeast Cig1. Oncogene. 8:2113–2118. 1993.PubMed/NCBI

19 

Okamoto K and Beach D: Cyclin G is a transcriptional target of the p53 tumor suppressor protein. EMBO J. 13:4816–4822. 1994.PubMed/NCBI

20 

Shang Y, Feng B, Zhou L, Ren G, Zhang Z, Fan X, Sun Y, Luo G, Liang J, Wu K, et al: The miR27b-CCNG1-P53-miR-508-5p axis regulates multidrug resistance of gastric cancer. Oncotarget. 7:538–549. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Fornari F, Gramantieri L, Giovannini C, Veronese A, Ferracin M, Sabbioni S, Calin GA, Grazi GL, Croce CM, Tavolari S, et al: MiR-122/cyclin G1 interaction modulates p53 activity and affects doxorubicin sensitivity of human hepatocarcinoma cells. Cancer Res. 69:5761–5767. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Liu X, Ma L, Rao Q, Mao Y, Xin Y, Xu H, Li C and Wang X: MiR-1271 inhibits ovarian cancer growth by targeting cyclin G1. Med Sci Monit. 21:3152–3158. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Huang Y, Shen XJ, Zou Q, Wang SP, Tang SM and Zhang GZ: Biological functions of microRNAs: A review. J Physiol Biochem. 67:129–139. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Kushlinskii NE, Fridman MV and Braga EA: Molecular mechanisms and microRNAs in osteosarcoma pathogenesis. Biochemistry (Mosc). 81:315–328. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Ram Kumar RM, Boro A and Fuchs B: Involvement and clinical aspects of microRNA in osteosarcoma. Int J Mol Sci. 17:E8772016. View Article : Google Scholar : PubMed/NCBI

26 

Ma C, Zhan C, Yuan H, Cui Y and Zhang Z: MicroRNA-603 functions as an oncogene by suppressing BRCC2 protein translation in osteosarcoma. Oncol Rep. 35:3257–3264. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Zeng G, Xun W, Wei K, Yang Y and Shen H: MicroRNA-27a-3p regulates epithelial to mesenchymal transition via targeting YAP1 in oral squamous cell carcinoma cells. Oncol Rep. 36:1475–1482. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Morales S, Gulppi F, Gonzalez-Hormazabal P, Fernandez-Ramires R, Bravo T, Reyes JM, Gomez F, Waugh E and Jara L: Association of single nucleotide polymorphisms in Pre-miR-27a, Pre-miR-196a2, Pre-miR-423, miR-608 and Pre-miR-618 with breast cancer susceptibility in a South American population. BMC Genet. 17:1092016. View Article : Google Scholar : PubMed/NCBI

29 

Danza K, Silvestris N, Simone G, Signorile M, Saragoni L, Brunetti O, Monti M, Mazzotta A, De Summa S, Mangia A, et al: Role of miR-27a, miR-181a and miR-20b in gastric cancer hypoxia-induced chemoresistance. Cancer Biol Ther. 17:400–406. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Liu F, Dear K, Huang L, Liu L, Shi Y, Nie S, Liu Y, Lu Y and Xiang H: Association between microRNA-27a rs895819 polymorphism and risk of colorectal cancer: A meta-analysis. Cancer Genet. 209:388–394. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Gao Y, Li BD and Liu YG: Effect of miR27a on proliferation and invasion in colonic cancer cells. Asian Pac J Cancer Prev. 14:4675–4678. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Chen DS, Zhu NL, Hung G, Skotzko MJ, Hinton DR, Tolo V, Hall FL, Anderson WF and Gordon EM: Retroviral vector-mediated transfer of an antisense cyclin G1 construct inhibits osteosarcoma tumor growth in nude mice. Hum Gene Ther. 8:1667–1674. 1997. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lin T, Ma Q, Zhang Y, Zhang H, Yan J and Gao C: MicroRNA-27a functions as an oncogene in human osteosarcoma by targeting CCNG1. Oncol Lett 15: 1067-1071, 2018.
APA
Lin, T., Ma, Q., Zhang, Y., Zhang, H., Yan, J., & Gao, C. (2018). MicroRNA-27a functions as an oncogene in human osteosarcoma by targeting CCNG1. Oncology Letters, 15, 1067-1071. https://doi.org/10.3892/ol.2017.7389
MLA
Lin, T., Ma, Q., Zhang, Y., Zhang, H., Yan, J., Gao, C."MicroRNA-27a functions as an oncogene in human osteosarcoma by targeting CCNG1". Oncology Letters 15.1 (2018): 1067-1071.
Chicago
Lin, T., Ma, Q., Zhang, Y., Zhang, H., Yan, J., Gao, C."MicroRNA-27a functions as an oncogene in human osteosarcoma by targeting CCNG1". Oncology Letters 15, no. 1 (2018): 1067-1071. https://doi.org/10.3892/ol.2017.7389
Copy and paste a formatted citation
x
Spandidos Publications style
Lin T, Ma Q, Zhang Y, Zhang H, Yan J and Gao C: MicroRNA-27a functions as an oncogene in human osteosarcoma by targeting CCNG1. Oncol Lett 15: 1067-1071, 2018.
APA
Lin, T., Ma, Q., Zhang, Y., Zhang, H., Yan, J., & Gao, C. (2018). MicroRNA-27a functions as an oncogene in human osteosarcoma by targeting CCNG1. Oncology Letters, 15, 1067-1071. https://doi.org/10.3892/ol.2017.7389
MLA
Lin, T., Ma, Q., Zhang, Y., Zhang, H., Yan, J., Gao, C."MicroRNA-27a functions as an oncogene in human osteosarcoma by targeting CCNG1". Oncology Letters 15.1 (2018): 1067-1071.
Chicago
Lin, T., Ma, Q., Zhang, Y., Zhang, H., Yan, J., Gao, C."MicroRNA-27a functions as an oncogene in human osteosarcoma by targeting CCNG1". Oncology Letters 15, no. 1 (2018): 1067-1071. https://doi.org/10.3892/ol.2017.7389
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