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The serum metabolic profiles of different Barcelona stages hepatocellular carcinoma associated with hepatitis B virus

  • Authors:
    • Lei Zhang
    • Guang‑Ye Wu
    • Yu‑Jing Wu
    • Shu‑Ye Liu
  • View Affiliations / Copyright

    Affiliations: Tianjin Key Laboratory of Artificial Cell, Artificial Cell Engineering Technology Research Center of Public Health Ministry, Tianjin Third Central Hospital, Tianjin 300170, P.R. China, Department of Clinical Laboratory, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 956-962
    |
    Published online on: November 13, 2017
       https://doi.org/10.3892/ol.2017.7393
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Abstract

The present study aimed to explore the characteristic ions distinguishing different Barcelona stages in patients with hepatitis B virus (HBV)‑associated hepatocellular carcinoma (HCC) using the ultra‑performance liquid chromatography‑mass spectrometry (UPLC‑MS) platform, and to evaluate their value in diagnosing and monitoring the progress of HCC. The serum was sampled from 20 healthy volunteers, 20 patients with HBV‑induced cirrhosis and 75 patients with HBV‑associated HCC of different BCLC stages. Samples were all examined using UPLC‑MS. Principal components analysis (PCA) and the orthogonal partial least squares discriminant analysis (OPLS‑DA) model were constructed to determine potential biomarkers. Then, the independent sample‑nonparametric test was used to perform the final screening for ion identification. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic value of these ions. Serum metabolomic PCA and OPLS‑DA models were established to diagnose different BCLC stages of HCC associated with HBV, with OPLS‑DA model parameters (R2X=67.2%, R2Y=82%, Q2Y=61.1%). A total of 20 metabolites with statistically significant differences among groups were identified, primarily including amino acids, bile acid, fatty acid and phosphatidate. The area under the curve (AUC) of LysoPC [18:2 (9Z,12Z)], LysoPC (P‑16:0), asparaginyl‑proline and vaccenic acid in the comparison between HCC and cirrhosis were all increased compared with that of AFP, indicating a more improved diagnosis ability. Furthermore, the AUC of L‑aspartyl‑4‑phosphate and LysoPC [20:5 (5Z,8Z,11Z,14Z,17Z)] in the stage A vs. B comparison were increased compared with that of AFP, but were decreased in the comparison between stage B and C. The present study succeeded in screening metabolic ions that reflect the progress of HCC with high diagnostic value. Thus, the identified ions may serve a role in clinically diagnosing HBV‑associated HCC and monitoring the development of the disease.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Wu GY, Wu YJ and Liu SY: The serum metabolic profiles of different Barcelona stages hepatocellular carcinoma associated with hepatitis B virus. Oncol Lett 15: 956-962, 2018.
APA
Zhang, L., Wu, G., Wu, Y., & Liu, S. (2018). The serum metabolic profiles of different Barcelona stages hepatocellular carcinoma associated with hepatitis B virus. Oncology Letters, 15, 956-962. https://doi.org/10.3892/ol.2017.7393
MLA
Zhang, L., Wu, G., Wu, Y., Liu, S."The serum metabolic profiles of different Barcelona stages hepatocellular carcinoma associated with hepatitis B virus". Oncology Letters 15.1 (2018): 956-962.
Chicago
Zhang, L., Wu, G., Wu, Y., Liu, S."The serum metabolic profiles of different Barcelona stages hepatocellular carcinoma associated with hepatitis B virus". Oncology Letters 15, no. 1 (2018): 956-962. https://doi.org/10.3892/ol.2017.7393
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Wu GY, Wu YJ and Liu SY: The serum metabolic profiles of different Barcelona stages hepatocellular carcinoma associated with hepatitis B virus. Oncol Lett 15: 956-962, 2018.
APA
Zhang, L., Wu, G., Wu, Y., & Liu, S. (2018). The serum metabolic profiles of different Barcelona stages hepatocellular carcinoma associated with hepatitis B virus. Oncology Letters, 15, 956-962. https://doi.org/10.3892/ol.2017.7393
MLA
Zhang, L., Wu, G., Wu, Y., Liu, S."The serum metabolic profiles of different Barcelona stages hepatocellular carcinoma associated with hepatitis B virus". Oncology Letters 15.1 (2018): 956-962.
Chicago
Zhang, L., Wu, G., Wu, Y., Liu, S."The serum metabolic profiles of different Barcelona stages hepatocellular carcinoma associated with hepatitis B virus". Oncology Letters 15, no. 1 (2018): 956-962. https://doi.org/10.3892/ol.2017.7393
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