Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
January-2018 Volume 15 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2018 Volume 15 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells

  • Authors:
    • Lina Sun
    • Lei Zhao
    • Dan Li
    • Shuai Xu
    • Xuexin Hou
    • Zhenjun Li
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China, Department of Molecular Physiology and Biophysics, Holden Comprehensive Cancer Center, University of Iowa Carver College of Medicine, Iowa, IA 52242, USA
  • Pages: 1157-1162
    |
    Published online on: November 15, 2017
       https://doi.org/10.3892/ol.2017.7417
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study assessed the subcellular localization and apoptotic potential of low‑risk human papilloma virus 6E6 (HPV‑6E6), expressed in bone marrow‑derived dendritic cells (DCs). DCs were obtained from C57BL/6 mice and transfected with a pGFP‑6E6 plasmid. The subcellular localization of E6 was determined by tracing green fluorescent protein (GFP) using fluorescence microscopy. Apoptosis was assessed by staining nuclei with DAPI and performing an apoptosis‑based flow cytometry assay. The co‑localization of E6 and p53 was also investigated using confocal microscopy. In addition, the expression of apoptosis‑associated proteins was analyzed using immunoblotting. The results of the present study demonstrated that low‑risk HPV‑6E6 is predominantly localized in the cytoplasm of DCs. Furthermore, p53 was upregulated in DCs transfected with pGFP‑6E6 and co‑localized with GFP‑6E6 in the cytoplasm. DCs transfected with a control pGFP plasmid did not undergo apoptosis, whereas cells transfected with pGFP‑6E6 did, as indicated by the presence of cell debris and condensation of the nuclei. Furthermore, the expression of apoptosis‑associated proteins, including p53, BCL2 associated X apoptosis regulator (Bax), BCL2 homologous antagonist/killer (Bak) and cytochrome c, were significantly higher in DCs expressing low‑risk E6 than in control cells. Therefore, the current study demonstrated that low‑risk HPV‑6E6 is predominantly located in the cytoplasm of DCs and induces apoptosis. This may be an important mechanism that explains why low‑risk HPV is unable to induce malignant transformation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Zur Hausen H: Papillomaviruses in human cancer. Cancer. 15:1692–1996. 1987.

2 

Kumvongpin R, Jearanaikool P, Wilailuckana C, Sae-Ung N, Prasongdee P, Daduang S, Wongsena M, Boonsiri P, Kiatpathomchai W, Swangvaree SS, et al: High sensitivity, loop-mediated isothermal amplification combined with colorimetric gold-nanoparticle probes for visual detection of high risk human papillomavirus genotypes 16 and 18. J Virol Methods. 234:90–95. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Shin TH, Pankhong P, Yan J, Sardesai NY and Weiner DB: Induction of robust cellular immunity against HPV6 and HPV11 in mice by DNA vaccine encoding for E6/E7 antigen. Hum Vaccin Immunother. 8:470–478. 2012. View Article : Google Scholar : PubMed/NCBI

4 

DasGupta T, Nweze EI, Yue H, Wang L, Jin J, Ghosh SK, Kawsar HI, Zender C, Androphy EJ, Weinberg A, et al: Human papillomavirus oncogenic E6 protein regulates human β-defensin 3 (hBD3) expression via the tumor suppressor protein p53. Oncotarget. 7:27430–27444. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Sun L, Zhang G, Lei T, Huang C, Song T and Si L: Two different HPV-11E6 fusion proteins trap p53 in the cytoplasm and induce apoptosis. Cancer Biol Ther. 7:1909–1915. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Liu Y, Chiriva-Internati M, Grizzi F, Salati E, Roman JJ, Lim S and Hermonat PL: Rapid induction of cytotoxic T-cell response against cervical cancer cells by human papillomavirus type 16 E6 antigen gene delivery into human dendritic cells by an adeno-associated virus vector. Cancer Gene Ther. 8:948–957. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Filippova M, Evans W, Aragon R, Filippov V, Williams VM, Hong L, Reeves ME and Duerksen-Hughes P: The small splice variant of HPV16 E6, E6, reduces tumor formation in cervical carcinoma xenografts. Virology 450–451. 1–164. 2014.

8 

Shi GN, Zhang CN, Xu R, Niu JF, Song HJ, Zhang XY, Wang WW, Wang YM, Li C, Wei XQ and Kong DL: Enhanced antitumor immunity by targeting dendritic cells with tumor cell lysate-loaded chitosan nanoparticles vaccine. Biomaterials. 113:191–202. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Baert T, Garg AD, Vindevogel E, VAN Hoylandt A, Verbist G, Agostinis P, Vergote I and Coosemans AN: In vitro generation of murine dendritic cells for cancer immunotherapy: An optimized protocol. Anticancer Res. 36:5793–5801. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Hiraiwa A, Kiyono T, Segwa K, Utsumi KR, Ohashi M and Ishibashi M: Comparative study on E6 and E7 genes of some cutaneous and genital papillomaviruses of human origin for their ability to transform 3Y1 cells. Virology. 192:102–111. 1993. View Article : Google Scholar : PubMed/NCBI

11 

Tao M, Kruhlak M, Xia S, Androphy E and Zheng ZM: Signals that dictate nuclear localization of human papillomavirus type 16 oncoprotein E6 in living cells. J Virol. 77:13232–13247. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Liang XH, Volkmann M, Klein R, Herman B and Lockett SJ: Co-localization of the tumor-suppressor protein p53 and human papillomavirus E6 protein in human cervical carcinoma cell lines. Oncogene. 8:2645–2652. 1993.PubMed/NCBI

13 

Kubicka-Sierszen A and Grzegorczyk JŁ: The influence of infectious factors on dendritic cell apoptosis. Arch Med Sci. 11:1044–1051. 2015.PubMed/NCBI

14 

Wang JJ, Li YF, Jin YY, Wang X and Chen TX: Effects of Epstein-Barr virus on the development of dendritic cells derived from cord blood monocytes: An essential role for apoptosis. Braz J Infect Dis. 16:19–26. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Kehmeier E, Rühl H, Voland B, Stöppler MC, Androphy E and Stöppler H: Cellular steady-state levels of ‘high risk’ but not ‘low risk’ human papillomavirus (HPV) E6 proteins are increased by inhibition of proteasome-dependent degradation independent of their p53 and E6AP binding capabilities. Virology. 299:72–87. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Li X and Coffino P: High-risk human papillomavirus E6 protein has two distinct binding sites within p53, of which only one determines degradation. J Virol. 70:4509–4516. 1996.PubMed/NCBI

17 

Martinez-Zapien D, Ruiz FX, Poirson J, Mitschler A, Ramirez J, Forster A, Cousido-Siah A, Masson M, Vande Pol S, Podjarny A, et al: Structure of the E6/E6AP/p53 complex required for HPV-mediated degradation of p53. Nature. 28:541–545. 2016. View Article : Google Scholar

18 

Huibregtse JM, Scheffner M and Howley PM: Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53. Mol Cell Biol. 13:775–784. 1993. View Article : Google Scholar : PubMed/NCBI

19 

Manjarrez ME, Ocadiz R, Valle L, Pacheco C, Marroquin A, De la Torre C, Selman M and Gariglio P: Detection of human papillomavirus and relevant tumor suppressors and oncoproteins in laryngeal tumors. Clin Cancer Res. 12:6946–6951. 2006. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sun L, Zhao L, Li D, Xu S, Hou X and Li Z: Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells. Oncol Lett 15: 1157-1162, 2018.
APA
Sun, L., Zhao, L., Li, D., Xu, S., Hou, X., & Li, Z. (2018). Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells. Oncology Letters, 15, 1157-1162. https://doi.org/10.3892/ol.2017.7417
MLA
Sun, L., Zhao, L., Li, D., Xu, S., Hou, X., Li, Z."Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells". Oncology Letters 15.1 (2018): 1157-1162.
Chicago
Sun, L., Zhao, L., Li, D., Xu, S., Hou, X., Li, Z."Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells". Oncology Letters 15, no. 1 (2018): 1157-1162. https://doi.org/10.3892/ol.2017.7417
Copy and paste a formatted citation
x
Spandidos Publications style
Sun L, Zhao L, Li D, Xu S, Hou X and Li Z: Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells. Oncol Lett 15: 1157-1162, 2018.
APA
Sun, L., Zhao, L., Li, D., Xu, S., Hou, X., & Li, Z. (2018). Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells. Oncology Letters, 15, 1157-1162. https://doi.org/10.3892/ol.2017.7417
MLA
Sun, L., Zhao, L., Li, D., Xu, S., Hou, X., Li, Z."Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells". Oncology Letters 15.1 (2018): 1157-1162.
Chicago
Sun, L., Zhao, L., Li, D., Xu, S., Hou, X., Li, Z."Low risk HPV‑6E6 induces apoptosis in bone marrow‑derived dendritic cells". Oncology Letters 15, no. 1 (2018): 1157-1162. https://doi.org/10.3892/ol.2017.7417
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team