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Article

Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model

  • Authors:
    • Shi Yu
    • Yue Hei
    • Weiping Liu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, No. 303 Hospital of Chinese People's Liberation Army, Nanning, Guangxi 530021, P.R. China, Department of Neurosurgery, The Fourth Military Medical University, Xi'an, Shaanxi 710000, P.R. China, Department of Neurosurgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 7443-7449
    |
    Published online on: December 7, 2017
       https://doi.org/10.3892/ol.2017.7543
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Abstract

The aim of the present study was to determine the roles of bone marrow mesenchymal stem cell (BM‑MSC) transplantation in a model of Alzheimer's disease (AD) and determine the underlying mechanism. The expression of selective Alzheimer's disease indicator‑1 (Seladin‑1) and nestin was detected using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The phosphoinositide 3‑kinase (PI3K) and extracellular signal‑regulated kinase (ERK)1/2 inhibitors, LY294002 and PD98059, were employed to evaluate the molecular mechanism. The results indicated that the mRNA and protein expression of Seladin‑1 and nestin was lower in the AD group when compared with the control group. BM‑MSC transplantation reversed this decrease in expression, potentially by increasing the protein expression of phosphorylated (p)‑protein kinase B (Akt) and p‑ERK1/2. In addition, LY294002 (the PI3K inhibitor) and/or PD98059 (the ERK1/2 inhibitor) blocked the enhancement of BM‑MSC transplantation on the expression of Seladin‑1 and nestin in the hippocampus. These results indicated that BM‑MSC transplantation enhanced Seladin‑1 and nestin expression potentially via a mechanism associated with the activation of the PI3K/Akt and ERK1/2 signaling pathways. The present study offers preliminary evidence that treatment with BM‑MSCs may represent a potential therapeutic approach against brain lesions in AD.
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Copy and paste a formatted citation
Spandidos Publications style
Yu S, Hei Y and Liu W: Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model. Oncol Lett 15: 7443-7449, 2018.
APA
Yu, S., Hei, Y., & Liu, W. (2018). Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model. Oncology Letters, 15, 7443-7449. https://doi.org/10.3892/ol.2017.7543
MLA
Yu, S., Hei, Y., Liu, W."Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model". Oncology Letters 15.5 (2018): 7443-7449.
Chicago
Yu, S., Hei, Y., Liu, W."Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model". Oncology Letters 15, no. 5 (2018): 7443-7449. https://doi.org/10.3892/ol.2017.7543
Copy and paste a formatted citation
x
Spandidos Publications style
Yu S, Hei Y and Liu W: Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model. Oncol Lett 15: 7443-7449, 2018.
APA
Yu, S., Hei, Y., & Liu, W. (2018). Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model. Oncology Letters, 15, 7443-7449. https://doi.org/10.3892/ol.2017.7543
MLA
Yu, S., Hei, Y., Liu, W."Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model". Oncology Letters 15.5 (2018): 7443-7449.
Chicago
Yu, S., Hei, Y., Liu, W."Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model". Oncology Letters 15, no. 5 (2018): 7443-7449. https://doi.org/10.3892/ol.2017.7543
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