Effects of NVP‑BEZ235, a dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitor, on HTLV-1-infected T-cell lines

  • Authors:
    • Chie Ishikawa
    • Masachika Senba
    • Naoki Mori
  • View Affiliations

  • Published online on: February 7, 2018     https://doi.org/10.3892/ol.2018.7979
  • Pages: 5311-5317
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Abstract

Adult T-cell leukemia (ATL) is an aggressive type of malignancy caused by human T‑cell leukemia virus type 1 (HTLV‑1). In ATL, the phosphatidylinositol 3‑kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway is constitutively active, promoting cell proliferation, survival and chemoresistance. Thus, the PI3K signaling pathway is an attractive therapeutic target for ATL. In the present study, the effects of RAD001 (an mTOR inhibitor), NVP‑BKM120 (a pan‑PI3K inhibitor) and NVP‑BEZ235 (a novel dual PI3K/mTOR inhibitor) on cultured HTLV‑1‑infected T‑cell lines were compared. The results demonstrated that NVP‑BEZ235 was more efficacious compared with RAD001 and NVP‑BKM120 at inhibiting cell growth. NVP‑BEZ235 exhibited cytostatic rather than cytotoxic effects on various HTLV‑1‑infected T‑cell lines, where it induced cell cycle arrest at G1 phase. NVP‑BEZ235 downregulated cyclin D1, cyclin D2, cyclin E, cyclin dependent kinase (CDK)2 and CDK4 expression, and the phosphorylation of retinoblastoma protein. In C.B‑17/Icr‑severe combined immune deficiency mice implanted with HTLV‑1‑infected HUT‑102 cells, oral NVP‑BEZ235 caused marked retardation of tumor growth compared with the control. The present in vitro and in vivo studies highlight the efficacious dual inhibition of PI3K, and mTOR following NVP‑BEZ235 treatment. Thus, the results of the current study provide preclinical rationale for phase I clinical studies to examine the effects of NVP-BEZ235 in patients with ATL.

Introduction

Adult T-cell leukemia (ATL) caused by the clonal proliferation of human T-cell leukemia virus type 1 (HTLV-1)-infected T cells (1) is highly resistant to currently available chemotherapies, and its prognosis is the worst among common subtypes of mature T/natural killer-cell neoplasms (2). Except for allogenic hematopoietic stem cell transplantation for some patients with ATL, no reliable treatment has yet been established (3).

Constitutive activation of phosphatidylinositol 3-kinase (PI3K) is linked to oncogenesis and disease progression in a variety of solid tumors and hematologic malignancies including ATL, apparently conferring drug resistance and resulting in poor prognosis (4). PI3K stimulates the production of phosphatidylinositol 3,4,5-triphosphate and subsequently the activation of 3-phosphoinositide-dependent protein kinase 1 (PDK1)/Akt and mammalian target of rapamycin (mTOR). This pathway integrates various extracellular stimuli to regulate several cellular processes, such as mRNA translation, cell survival and cell cycle progression (5). The inactive phosphorylated form of phosphatase and tensin homologue (PTEN), a negative PI3K regulator, are often expressed on ATL cells and contribute to constitutive PI3K signaling (6). These pathways could be suitable targets for new therapies for ATL. Evidence suggests that PI3K-selective targeting can potentially suppress this pathway. In this regard, several types of PI3K inhibitors are currently being investigated (7), including, NVP-BKM120, which is reported to be effective against several types of malignancies in cell- (4,810) and laboratory animal-based studies (11).

On the other hand, allosteric mTOR inhibitors, such as rapamycin and its analogues (temsirolimus and everolimus; earlier code name RAD001), mainly target mTOR complex 1 (mTORC1), and the former are approved for use in various cancers (12). However, the mTORC1-specificity of rapamycin analogues can limit their efficacies based on the feedback activation of upstream PI3K signaling, resulting in Akt hyperactivation through mTORC2 (13). Targeting the PI3K/Akt/mTOR pathway at multiple levels may therefore provide a more effective antitumor activity than selective inhibition of only one component of the pathway. Unlike rapamycin and its analogues, the new generations of dual PI3K/mTOR inhibitors competitively inhibit ATP and target the catalytic site of both kinases (7). These inhibitors have more potent antitumor effects and can suppress the prosurvival regulatory feedback (14). Oral NVP-BEZ235 is reported to inhibit the kinase activities of mTORC1, mTORC2 and PI3K (15). Several pre-clinical studies reported the efficacy of NVP-BEZ235 in various lymphoid malignancies (14,1618) and its effects, either alone or in combination with other compounds, are currently being tested in the treatment of various other malignancies (12).

The aim of the present study was to compare the effects of NVP-BEZ235 with those of NVP-BKM120 (pan-class I PI3K inhibitor) and RAD001 (mTORC1 inhibitor) at a cellular level using HTLV-1-infected T cells. In addition, we also tested the effects of NVP-BEZ235 in laboratory animals harboring ATL tumors. The results suggest that suppression of both PI3K and mTORC2, in addition to mTORC1, is more efficacious in the treatment of ATL, compared with inhibition of each of these kinases.

Materials and methods

Inhibitors

NVP-BEZ235, NVP-BKM120 and RAD001 were kindly gifted by Novartis Institutes for BioMedical Research (Basel, Switzerland). Each was dissolved in dimethyl sulfoxide (cat. no. 13407-45; Nacalai Tesque, Inc., Kyoto, Japan) to create stock solution that was stored at −80°C.

Cell culture

HTLV-1-transformed T-cell lines, MT-2, MT-4, HUT-102 and SLB-1, and ATL-derived T-cell lines, TL-OmI and ED-40515(−), were maintained in RPMI-1640 medium (cat. no. 30264-56; Nacalai Tesque, Inc.) supplemented with 10% heat-inactivated fetal bovine serum (Biological Industries, Kibbutz Beit Haemek, Israel) and 1% penicillin/streptomycin (cat. no. 09367-34; Nacalai Tesque, Inc.).

Assessment of cell proliferation and cytotoxicity

Cell proliferation and cytotoxicity were measured by the water-soluble tetrazolium (WST)-8 assay (cat. no. 07553-44; Nacalai Tesque, Inc.). The WST-8 is taken up by viable cells and reduced to a colored formazan product by mitochondrial dehydrogenase (19). For the WST-8 assay, cells were treated with increasing concentrations of the test drug for 48 h. Then, WST-8 was added to each well and incubated. Absorbance was measured at 450 nm by a 680 XR microplate absorbance reader (Bio-Rad Laboratories, Inc., Hercules, CA, USA). All experiments were conducted in triplicates. The drug concentration required for 50% inhibition of cell proliferation (IC50) was evaluated with CalcuSyn software (version 2.0; Biosoft, Cambridge, UK).

Cell cycle analysis

Cell cycle was analyzed using the CycleTEST Plus DNA Reagent kit (cat. no. 340242; Becton-Dickinson Immunocytometry Systems, San Jose, CA, USA) and an Epics XL flow cytometer (Beckman Coulter, Inc., Brea, CA, USA). The percentages of cells in each cell cycle phase were calculated using MultiCycle software (version 3.0; Phoenix Flow Systems, San Diego, CA, USA).

Apoptosis assay

Apoptosis was quantified using the APO2.7 assay. Cells were stained with phycoerythrin-conjugated APO2.7 antibody (cat. no. IM2088; Beckman Coulter, Marseille, France). This antibody is reported to specifically detect a 38-kDa mitochondrial membrane antigen (7A6), which is expressed during programmed cell death (20). Events were acquired using a flow cytometer.

Western blot analysis

After drug treatment, cells were harvested and lysed in a buffer solution [composition: 62.5 mM Tris-HCl (pH 6.8) (cat. no. 35434-21; Nacalai Tesque, Inc.), 2% sodium dodecyl sulfate (cat. no. 31607-65; Nacalai Tesque, Inc.), 10% glycerol (cat. no. 17045-65; Nacalai Tesque, Inc.), 6% 2-mercaptoethanol (cat. no. 21438-82; Nacalai Tesque, Inc.) and 0.01% bromophenol blue (cat. no. 021-02911; Wako Pure Chemical Industries, Osaka, Japan)]. The cell lysates (20 µg/lane) were electrophoresed on sodium dodecyl sulfate-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (cat. no. IPVH00010EMD; Merck KGaA, Darmstadt, Germany). The membranes were immunoblotted with the following primary antibodies: Total Akt (cat. no. 9272), phospho-Akt (Ser473; cat. no. 4060), phospho-Akt (Thr308; cat. no. 13038), phospho-PDK1 (Ser241; cat. no. 3061), phospho-p70 S6 kinase (S6K) (Thr421 and Ser424; cat. no. 9204) and phospho-4E-BP1 (Thr70; cat. no. 9455) were all from Cell Signaling Technology, Inc. (Beverly, MA, USA); cyclin D2 (cat. no. sc-593) was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); cyclin D1 (cat. no. K0062-3) and phospho-retinoblastoma protein (pRb) (Ser780; cat. no. M054-3S) were from Medical & Biological Laboratories, Co., (Aichi, Japan); cyclin E (cat. no. MS-870), cyclin dependent kinase (CDK)2 (cat. no. MS-617), CDK4 (cat. no. MS-299), CDK6 (cat. no. MS-398) and actin (cat. no. MS-1295) were from Neomarkers, Inc. (Fremont, CA, USA). Then, the membranes were incubated with species-specific horseradish peroxidase-conjugated secondary antibody (cat. nos. 7074 and 7076) from Cell Signaling Technology, Inc.

Xenograft tumor model

In these experiments, 5-week-old female C.B-17/Icr-severe combined immune deficiency (SCID) mice (Kyudo, Co., Tosu, Japan) were kept in specific pathogen-free conditions, housed in air-conditioned rooms (temperature, 24°C; humidity, 60%) set at 12/12 h light/dark cycles, and fed standard rodent diet (CE-2 from CLEA Japan, Inc., Tokyo, Japan) and provided with water ad libitum. A total of 1×107 HUT-102 cells (suspended in 300 µl of RPMI-1640 medium) were injected subcutaneously behind the ears. The tumor-bearing mice were divided at random into two treatment groups (n=6 each). NVP-BEZ235 was solubilized in 0.5% methylcellulose and administrated at 40 mg/kg body weight/day by oral gavage, and the treatment was continued for 28 days, starting the day after cell injection. The control group was treated with vehicle only. Tumor volume was estimated by measuring tumor diameter once every week with a caliper. The ellipsoid volume formula (π/6 × length × width × height) was used (21). The animals were weighed once every week, and then sacrificed at day 28. This was followed by excision of the tumor for analysis (see below). The present study was approved by the Animal Care and Use Committee (reference no. 5276) and performed according to the Guidelines for Animal Experimentation of our university.

Histopathological examination and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL) assay

Tumor tissues were fixed in formalin (Wako Pure Chemical Industries), dehydrated using ethanol (Japan Alcohol Selling Co., Tokyo, Japan) solution and embedded in paraffin (cat. no. 09620; Sakura Finetek Japan Co., Tokyo, Japan). The paraffin-embedded ATL specimens were stained with hematoxylin and eosin (H&E; cat. nos. 234-12 and 1159350025; Merck KGaA) and examined under a microscope. DNA fragmentation was analyzed by TUNEL kit (cat. no. 11684817910; Roche Applied Science, Penzberg, Germany). Cells were examined under Axioskop-2 Plus light microscope equipped with an Achroplan lens (Zeiss, Hallbergmoos, Germany). Images were acquired with appropriate software (AxioCam 503 color and AxioVision LE64; Zeiss GmbH, Jena, Germany).

Measurements of soluble interleukin-2 receptor (sIL-2R) and soluble cluster of differentiation 30 (sCD30)

Using the appropriate enzyme-linked immunosorbent assay (ELISA) kit, we measured serum levels of human sIL-2R (cat. no. DR2A00; R&D Systems, Inc., Minneapolis, MN, USA) and human sCD30 (cat. no. SK00582-01; Aviscera Bioscience, Inc., Santa Clara, CA, USA), according to the instructions provided by the supplier.

Statistical analysis

All values are expressed as the mean ± standard deviation (SD). The unpaired t-test was used to test the statistical differences between the two treatment groups. P<0.05 was considered to indicate a statistically significant difference.

Results

Superior growth inhibitory activity of NVP-BEZ235 compared to NVP-BKM120 and RAD001 in HTLV-1-infected T-cell lines

Considering that PI3K is upstream of mTOR, and is activated in ATL and HTLV-1-infected T-cell lines (6,22), we considered that inhibition of PI3K in addition to mTOR would be a superior strategy to inhibition of mTOR or PI3K alone. We compared the effects of the dual PI3K/mTOR inhibitor NVP-BEZ235 with those of the PI3K inhibitor NVP-BKM120 and the mTOR inhibitor RAD001. To determine the IC50 values, HTLV-1-transformed T-cell lines MT-2, MT-4, HUT-102, SLB-1, and patient-derived ATL cell lines TL-OmI and ED-40515(−) were cultured with increasing concentrations of NVP-BEZ235, NVP-BKM120 and RAD001 (1–1,000 nM) for 48 h (Fig. 1A-C). Table I shows the IC50 values obtained for NVP-BEZ235 and NVP-BKM120 in HTLV-1-infected T-cell lines. Exposure of these cells to NVP-BEZ235 and NVP-BKM120 inhibited the growth of HTLV-1-infected T-cell lines in a dose-dependent manner with IC50 from 11.9 to 293.0 nM (NVP-BEZ235) and from 397.2 to 2,248.6 nM (NVP-BKM120), as measured by WST-8 assay. Interestingly, lower nanomolar concentrations of NVP-BEZ235 were needed to inhibit the proliferation of HTLV-1-infected T-cell lines compared with NVP-BKM120. RAD001 reduced the proliferation of HTLV-1-infected T-cell lines at low nanomolar concentrations, but increasing doses of RAD001 did not significantly enhance the anti-proliferative activity. The IC50 was not achieved. We also evaluated the effects of the drugs using samples of peripheral blood mononuclear cells (PBMC) obtained from a healthy subject (Fig. 1A-C). NVP-BEZ235, NVP-BKM120 and RAD001 each had minimal effect on PBMC from the healthy donor. Fig. 1D shows the effects of 100 nM of NVP-BEZ235, NVP-BKM120 and RAD001 on cell proliferation in HTLV-1-infected T-cell lines and PBMC from the healthy donor. Cell proliferation in all HTLV-1-infected T-cell lines was most effectively inhibited with NVP-BEZ235. These results indicate that the dual PI3K/mTOR inhibitor is a more potent suppressor of proliferation of HTLV-1-infected T-cell lines, compared with each compound alone.

Table I.

The IC50 values for HTLV-1-infected T-cell lines and PBMC treated with NVP-BEZ235 and NVP-BKM120 for 48 h.

Table I.

The IC50 values for HTLV-1-infected T-cell lines and PBMC treated with NVP-BEZ235 and NVP-BKM120 for 48 h.

Cell lineNVP-BEZ235 (nM)NVP-BKM120 (nM)
MT-2  40.1   672.3
MT-4293.0   616.3
SLB-1178.32,248.6
HUT-102  11.9   397.2
TL-OmI  29.52,221.6
ED-40505(−)  54.61,117.2
PBMC>15,000>6,000

[i] HTLV-1, human T-cell leukemia virus type 1; PBMC, peripheral blood mononuclear cells.

NVP-BEZ235 induces cell cycle arrest

For a better understanding of the mechanism of NVP-BEZ235-induced inhibition of HTLV-1-infected T-cell line growth, cell cycle changes were examined (Fig. 2A). We employed propidium iodide staining and DNA content analysis by flow cytometry to determine whether 24 h of treatment with NVP-BEZ235 altered cell cycle progression. A high percentage of cells in the G0/G1 phase of the cell cycle, with a concomitant decrease in the proportion of those in the S phase, was noted in the presence of NVP-BEZ235, as the concentrations increased. Our findings indicate that NVP-BEZ235 inhibits the growth of HTLV-1-infected T-cell lines by inducing cell cycle arrest at the G0/G1 phase.

Effects of NVP-BEZ235 on apoptosis

To evaluate whether the anti-ATL activity of NVP-BEZ235 was due to induction of apoptosis, flow cytometric analysis was performed. MT-4 and HUT-102 cells were treated with different concentrations of NVP-BEZ235 for 24–48 h and apoptosis was assessed by APO2.7 staining. As shown in Fig. 2B, flow cytometric analysis demonstrated a slight increase in apoptotic cells after treatment with NVP-BEZ235, suggesting that the reduction in WST-8 activity was primarily due to inhibition of cell proliferation.

Effects of NVP-BEZ235 on cell cycle regulators

pRb controls cell cycle progression at the G0/G1 to S transition in response to certain signals for growth inhibition (23). To determine the mechanism by which NVP-BEZ235 induces G0/G1 cell cycle arrest, the phosphorylation status of pRb was evaluated by western blot analysis. The level of the phosphorylated form of pRb was decreased in cells treated with NVP-BEZ235 (Fig. 3A and B). pRb is phosphorylated by catalytic subunits of CDK4/CDK6 and CDK2 complexed with specific regulatory subunits of cyclins D and E, respectively. The expression levels subtypes of D-type cyclins, cyclin D1 and cyclin D2, examined by western blot analysis, were decreased in MT-4, HUT-102 and TL-OmI cells following treatment with NVP-BEZ235. Furthermore, the levels of CDK4 and/or CDK6 were also decreased in NVP-BEZ235-treated HUT-102 and TL-OmI cells. Moreover, western blot analysis indicated that treatment with NVP-BEZ235 downregulated the expression of cyclin E and CDK2 in MT-4, HUT-102 and TL-OmI cells (Fig. 3A and B).

NVP-BEZ235 inhibits PI3K/Akt/mTOR pathway

Next, we examined the effects of NVP-BEZ235 on the PI3K/Akt/mTOR signaling pathway. To confirm the efficacy of NVP-BEZ235 against downstream targets of PI3 K and mTOR, we tested the phosphorylation status of the downstream targets of these two kinases, using western blot analysis (Fig. 3C). Treatment with NVP-BEZ235 for 24–48 h induced marked reduction in phosphorylated PDK1 and Akt at Thr308, both targets of the kinase activity of PI3K. Reduced phosphorylation of p70 S6K and 4E-BP1, both targets of mTOR, reflects inhibition of mTOR by NVP-BEZ235. Importantly, the reduced phosphorylation of Akt at Ser473, a feedback phosphorylation substrate of mTORC2, indicates that NVP-BEZ235 also inhibits mTORC2 activity (Fig. 3C). Treatment did not affect total Akt protein level at any dose. Thus, dual inhibition of PI3K and mTOR seems to contribute to decreased cell proliferation by NVP-BEZ235.

Efficacy of NVP-BEZ235 in SCID mouse model of ATL

Mice engrafted with HUT-102 cells were treated daily for 4 weeks with NVP-BEZ235 at 40 mg/kg/body weight. NVP-BEZ235 resulted in reduction of body weight after 4 weeks of treatment (21.2 vs. 22.3 g, P<0.05). A decrease in body weight was observed in NVP-BEZ235-treated mice (maximum loss, 6.5%) compared with the control mice at week one; however, the growth curve of the NVP-BEZ235 group was similar to that of the control group thereafter. Multiple tumors were not observed in any of the mice. Treatment with NVP-BEZ235 also resulted in inhibition of tumor growth, which became significant after 14, 21 and 28 days (Fig. 4A). A statistically significant inhibition of tumor weight was also observed (Fig. 4B and C, left panel). No macroscopic metastases and ascites were observed in mice following sacrifice at day 28, thus suggesting that the increase in body weight after week one in NVP-BEZ235-treated mice was due to natural growth (data not shown). To validate the results of the in vivo xenograft model, the effects of NVP-BEZ235 on sIL-2R (24) and sCD30 (25) were examined using serum samples obtained from SCID mice. The serum level of sIL-2R and sCD30 in the treated mice was lower than that of the control mice at 4 weeks after initiation of therapy, albeit statistically insignificant (Fig. 4C, middle and right panels). Finally, H&E staining of tumor sections showed apoptosis of tumor cells, which was identified by condensation of the cytoplasm, fragmentation of the cell nuclei, and hyperchromatism and condensation of the chromatin (Fig. 4D, left panel). Furthermore, TUNEL staining identified numerous apoptotic cells in the tumors of the NVP-BEZ235-treated group (Fig. 4D, right panel).

Discussion

The PI3K/Akt/mTOR pathway plays an important role in cell proliferation, and is thus an interesting target for the treatment of various cancers, including hematological malignancies (26). Loss of function of phosphatases, most notably PTEN, which regulate this pathway, has been demonstrated in ATL (6). It has been demonstrated that inhibition of signaling through mTORC1 inhibits growth of ATL cells (22). Although preclinical studies have reported that mTOR inhibitors can suppress tumor cell proliferation, monotherapy using these agents has not been as efficacious as initially expected (27). In this regard, mTORC1 inhibition can result in mTORC2-induced phosphorylation of Akt on Ser473 based on loss of the negative feedback loop through S6K, in addition to further activation of PI3K (13). Based on this background, we hypothesized that dual PI3K/mTOR inhibition could be superior to mTORC1 inhibition alone. In this study, we compared the efficacy of NVP-BEZ235 (a dual ATP-competitive PI3K/mTOR inhibitor), with RAD001 (a rapamycin analogue and specific inhibitor of mTORC1), and NVP-BKM120 (an ATP-competitive pan-PI3K inhibitor), in HTLV-1-infected T-cell lines.

Our results showed that RAD001 suppressed the growth of all HTLV-1-infected T-cell lines and that only low concentrations were required to inhibit the proliferation of sensitive cell lines; higher doses of RAD001 did not augment its efficacy. These findings suggest that effective anti-ATL protocols should include other compounds, in addition to RAD001. In contrast, treatment of HTLV-1-infected T-cell lines with NVP-BEZ235 and NVP-BKM120 led dose-dependently to a much larger decrease in cell growth. Based on the IC50 value of NVP-BEZ235 within the nanomolar range, NVP-BEZ235 was more potent in terms of growth inhibition than the selective inhibitor of PI3K. The poor anti-ATL activity of NVP-BKM235 and RAD001 at nanomolar concentrations might be due to their inability to block mTORC1 and mTORC2. Another potential factor is the limited suppressive effect of allosteric inhibitor on mTORC1, resulting in compensatory activation of feedback loops. However, NVP-BEZ235 downregulated phosphorylated PDK1 and Akt expression and downstream signaling of mTOR (phospho-4E-BP1 and phospho-p70-S6K).

We also showed that NVP-BEZ235 induced cell cycle arrest at the G0/G1 phase and slightly activated the apoptotic pathways. NVP-BEZ235 treatment inhibited pRb phosphorylation and cyclin D1, D2, E and CDK2, 4, 6 expressions in cells; consequently, it is highly possible that NVP-BEZ235 treatment causes G0/G1 arrest by blocking cyclin D-CDK4/6 and cyclin E-CDK2 complexes-mediated pRb phosphorylation, which is critical to the progression of the cell cycle at the G0/G1 to S transition. The effectiveness of this agent was confirmed in xenograft models of ATL. NVP-BEZ235 markedly suppressed tumor growth in these mice compared with control group. To our knowledge, this is the first study to show that the novel dual PI3K/mTOR inhibitor NVP-BEZ235 is effective against ATL, both in vitro and in vivo. Therapies targeting the PI3K/Akt/mTOR pathway may therefore be of interest for ATL and we believe that they are promising candidates for ATL therapy.

In conclusion, simultaneous inhibition of PI3K, mTORC1 and mTORC2 by the dual inhibitor NVP-BEZ235 showed profound anti-ATL activity. Our data provide a strong preclinical rationale for phase I clinical studies to test this compound in patients with ATL.

Acknowledgements

The authors thank Dr Michiyuki Maeda for ED-40515(−), the Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc. (Okayama, Japan) for HUT-102, and Novartis Institutes for BioMedical Research (Basel, Switzerland) for the kind supply of NVP-BEZ235, NVP-BKM120 and RAD001. This study was supported in part by two grants from JSPS KAKENHI (nos. 15K18414 and 25461428).

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April-2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ishikawa C, Senba M and Mori N: Effects of NVP‑BEZ235, a dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitor, on HTLV-1-infected T-cell lines. Oncol Lett 15: 5311-5317, 2018
APA
Ishikawa, C., Senba, M., & Mori, N. (2018). Effects of NVP‑BEZ235, a dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitor, on HTLV-1-infected T-cell lines. Oncology Letters, 15, 5311-5317. https://doi.org/10.3892/ol.2018.7979
MLA
Ishikawa, C., Senba, M., Mori, N."Effects of NVP‑BEZ235, a dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitor, on HTLV-1-infected T-cell lines". Oncology Letters 15.4 (2018): 5311-5317.
Chicago
Ishikawa, C., Senba, M., Mori, N."Effects of NVP‑BEZ235, a dual phosphatidylinositol 3-kinase/mammalian target of rapamycin inhibitor, on HTLV-1-infected T-cell lines". Oncology Letters 15, no. 4 (2018): 5311-5317. https://doi.org/10.3892/ol.2018.7979