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Clinical significance of high expression of miR-452-5p in lung squamous cell carcinoma

  • Authors:
    • Xiao‑Ning Gan
    • Ting‑Qing Gan
    • Rong‑Quan He
    • Jie Luo
    • Rui‑Xue Tang
    • Han‑Lin Wang
    • Hong Zhou
    • Hui Qing
    • Jie Ma
    • Xiao‑Hua Hu
    • Gang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Medical Oncology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China
    Copyright: © Gan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6418-6430
    |
    Published online on: February 20, 2018
       https://doi.org/10.3892/ol.2018.8088
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Abstract

The role of microRNA (miRNA)-452-5p in lung squamous cell carcinoma (LUSC) remains unclear. Therefore, the present systematic study was performed to investigate the clinical significance and the rudimentary mechanism of the function of miR‑452‑5p in LUSC. The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were utilized to confirm the expression level and clinical value of miR‑452‑5p in LUSC. Using online databases and bioinformatic software, gene ontology (GO), pathway and protein‑protein interaction (PPI) analyses of miR‑452‑5p target genes were performed to examine the molecular mechanism of miR‑452‑5p. The association between the expression of miR‑452‑5p and that of its hub genes was verified using TCGA. Based on TCGA data on 387 clinical specimens, the expression of miR‑452‑5p in LUSC was significantly increased compared with adjacent lung tissues (7.1525±1.39063 vs. 6.0885±0.35298; P<0.001). The expression levels of miR‑452‑5p were significantly correlated with age (P=0.001) and tumor‑node metastasis stage (P=0.028). Furthermore, the increased expression of miR‑452‑5p in LUSC compared with non‑cancerous tissue [standard mean deviation (SMD), 0.372; 95% confidence interval (CI), 0.020‑0.724; z=2.07; P=0.038] was validated by a meta‑analysis of 720 clinical samples. The GO and pathway analyses revealed that miR‑452‑5p target genes were mainly enriched in the ʻregulation of transcription’, ʻnucleoplasm’, ʻprotein binding’ and ʻcell cycle’ pathways. A total of 10 hub genes were identified by PPI analysis, and 5 hub genes (SMAD4, SMAD2, CDKN1B, YWHAE and YWHAB) were significantly enriched in the ʻcell cycle’ pathway. The expression of CDKN1B was negatively correlated with miR‑452‑5p (P=0.003). It was concluded that miR‑452‑5p may serve an essential role in the occurrence and progression of LUSC by targeting CDKN1B, which is involved in the cell cycle.
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Copy and paste a formatted citation
Spandidos Publications style
Gan XN, Gan TQ, He RQ, Luo J, Tang RX, Wang HL, Zhou H, Qing H, Ma J, Hu XH, Hu XH, et al: Clinical significance of high expression of miR-452-5p in lung squamous cell carcinoma. Oncol Lett 15: 6418-6430, 2018.
APA
Gan, X., Gan, T., He, R., Luo, J., Tang, R., Wang, H. ... Chen, G. (2018). Clinical significance of high expression of miR-452-5p in lung squamous cell carcinoma. Oncology Letters, 15, 6418-6430. https://doi.org/10.3892/ol.2018.8088
MLA
Gan, X., Gan, T., He, R., Luo, J., Tang, R., Wang, H., Zhou, H., Qing, H., Ma, J., Hu, X., Chen, G."Clinical significance of high expression of miR-452-5p in lung squamous cell carcinoma". Oncology Letters 15.5 (2018): 6418-6430.
Chicago
Gan, X., Gan, T., He, R., Luo, J., Tang, R., Wang, H., Zhou, H., Qing, H., Ma, J., Hu, X., Chen, G."Clinical significance of high expression of miR-452-5p in lung squamous cell carcinoma". Oncology Letters 15, no. 5 (2018): 6418-6430. https://doi.org/10.3892/ol.2018.8088
Copy and paste a formatted citation
x
Spandidos Publications style
Gan XN, Gan TQ, He RQ, Luo J, Tang RX, Wang HL, Zhou H, Qing H, Ma J, Hu XH, Hu XH, et al: Clinical significance of high expression of miR-452-5p in lung squamous cell carcinoma. Oncol Lett 15: 6418-6430, 2018.
APA
Gan, X., Gan, T., He, R., Luo, J., Tang, R., Wang, H. ... Chen, G. (2018). Clinical significance of high expression of miR-452-5p in lung squamous cell carcinoma. Oncology Letters, 15, 6418-6430. https://doi.org/10.3892/ol.2018.8088
MLA
Gan, X., Gan, T., He, R., Luo, J., Tang, R., Wang, H., Zhou, H., Qing, H., Ma, J., Hu, X., Chen, G."Clinical significance of high expression of miR-452-5p in lung squamous cell carcinoma". Oncology Letters 15.5 (2018): 6418-6430.
Chicago
Gan, X., Gan, T., He, R., Luo, J., Tang, R., Wang, H., Zhou, H., Qing, H., Ma, J., Hu, X., Chen, G."Clinical significance of high expression of miR-452-5p in lung squamous cell carcinoma". Oncology Letters 15, no. 5 (2018): 6418-6430. https://doi.org/10.3892/ol.2018.8088
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