A radioresistant fraction of acute promyelocytic leukemia cells exhibit CD38 cell-surface antigen and mRNA expression

Monzen,Satoru; Chiba,Mitsuru; Ueno,Tatsuya; Morino,Yuki; Terada,Kenji; Yamaya,Hiroki; Hosokawa,Yoichiro

doi:10.3892/ol.2018.8099

A radioresistant fraction of acute promyelocytic leukemia cells exhibit CD38 cell-surface antigen and mRNA expression

Authors:
- Satoru Monzen
- Mitsuru Chiba
- Tatsuya Ueno
- Yuki Morino
- Kenji Terada
- Hiroki Yamaya
- Yoichiro Hosokawa
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Affiliations: Department of Radiation Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan, Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan
Published online on: February 21, 2018 https://doi.org/10.3892/ol.2018.8099
Pages: 6709-6714

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Abstract

In the present study, the cell viability and cluster of differentiation (CD)38 mRNA expression were evaluated in radioresistant (Res)-HL60 acute promyelocytic leukemia (APL) cells. Cell viability in Res‑HL60 cells was higher compared with wild‑type HL60 cells, but did not differ between high and mid/low CD38 antigen expression groups in Res‑HL60 cells. A higher expression of CD38 mRNA in Res‑HL60 cells was observed, particularly in the CD38high cell subpopulation. Furthermore, the expression of CD38 mRNA was upregulated following exposure to X‑irradiation. In contrast, the characteristic expression of CD45 and CCAAT/enhancer‑binding protein α mRNA were not altered. These results suggest that the accumulation of CD38 protein in radioresistant APL cells, resulting from the constant expression of CD38 mRNA induced by X‑irradiation, is a characteristic response of the radioresistant‑surviving fraction; however, the accumulation of CD38 did not influence the extent of radioresistant behavior.

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1	Ariyoshi K, Takabatake T, Shinagawa M, Kadono K, Daino K, Imaoka T, Kakinuma S, Nishimura M and Shimada Y: Age dependence of hematopoietic progenitor survival and chemokine family gene induction after gamma irradiation in bone marrow tissue in C3H/He mice. Radiat Res. 181:302–13. 2014. View Article : Google Scholar : PubMed/NCBI
2	Monzen S, Yoshino H, Kasai-Eguchi K and Kashiwakura I: Characteristics of myeloid differentiation and maturation pathway derived from human hematopoietic stem cells exposed to different linear energy transfer radiation types. PLoS One. 8:e593852013. View Article : Google Scholar : PubMed/NCBI
3	Haro KJ, Scott AC and Scheinberg DA: Mechanisms of resistance to high and low linear energy transfer radiation in myeloid leukemia cells. Blood. 120:2087–2097. 2012. View Article : Google Scholar : PubMed/NCBI
4	de Berranger E, Cousien A, Petit A, Peffault de Latour R, Galambrun C, Bertrand Y, Salmon A, Rialland F, Rohrlich PS, Vannier JP, et al: Impact on long-term OS of conditioning regimen in allogeneic BMT for children with AML in first CR: TBI+CY versus BU+CY: A report from the Société Française de Greffe de Moelle et de Thérapie Cellulaire. Bone Marrow Transplant. 49:382–388. 2014. View Article : Google Scholar : PubMed/NCBI
5	Willemze AJ, Geskus RB, Noordijk EM, Kal HB, Egeler RM and Vossen JM: HLA-identical haematopoietic stem cell transplantation for acute leukemia in children: less relapse with higher biologically effective dose of TBI. Bone Marrow Transplant. 40:319–327. 2007. View Article : Google Scholar : PubMed/NCBI
6	Monzen S, Takimura K, Kashiwakura I and Hosokawa Y: Acute promyelocytic leukemia mutated to radioresistance suppressed monocyte lineage differentiation by phorbol 12-myristate 13-acetate. Leuk Res. 37:1162–1169. 2013. View Article : Google Scholar : PubMed/NCBI
7	Hazawa M, Hosokawa Y, Monzen S, Yoshino H and Kashiwakura I: Regulation of DNA damage response and cell cycle in radiation-resistant HL60 myeloid leukemia cells. Oncol Rep. 28:55–61. 2012.PubMed/NCBI
8	Kashiwakura I, Kuwabara M, Inanami O, Murakami M, Hayase Y, Takahashi TA and Takagi Y: Radiation sensitivity of megakaryocyte colony-forming cells in human placental and umbilical cord blood. Radiat Res. 153:144–152. 2000. View Article : Google Scholar : PubMed/NCBI
9	Yuan JS, Wang D and Stewart CN Jr: Statistical methods for efficiency adjusted real-time PCR quantification. Biotechnol J. 3:112–123. 2008. View Article : Google Scholar : PubMed/NCBI
10	Takasawa S, Tohgo A, Noguchi N, Koguma T, Nata K, Sugimoto T, Yonekura H and Okamoto H: Synthesis and hydrolysis of cyclic ADP-ribose by human leukocyte antigen CD38 and inhibition of the hydrolysis by ATP. J Biol Chem. 268:26052–26054. 1993.PubMed/NCBI
11	Petzer AL, Hogge DE, Landsdorp PM, Reid DS and Eaves CJ: Self-renewal of primitive human hematopoietic cells (long-term-culture-initiating cells) in vitro and their expansion in defined medium. Proc Natl Acad Sci USA. 93:pp. 1470–1474. 1996; View Article : Google Scholar : PubMed/NCBI
12	Charbonneau H, Tonks NK, Walsh KA and Fischer EH: The leukocyte common antigen (CD45): A putative receptor-linked protein tyrosine phosphatase. Proc Natl Acad Sci USA. 85:pp. 7182–7186. 1988; View Article : Google Scholar : PubMed/NCBI
13	Pabst T, Mueller BU, Harakawa N, Schoch C, Haferlach T, Behre G, Hiddemann W, Zhang DE and Tenen DG: AML1-ETO downregulates the granulocytic differentiation factor C/EBPalpha in t(8;21) myeloid leukemia. Nat Med. 7:444–451. 2001. View Article : Google Scholar : PubMed/NCBI
14	Yamamori T, Yasui H, Yamazumi M, Wada Y, Nakamura Y, Nakamura H and Inanami O: Ionizing radiation induces mitochondrial reactive oxygen species production accompanied by upregulation of mitochondrial electron transport chain function and mitochondrial content under control of the cell cycle checkpoint. Free Radic Biol Med. 53:260–270. 2012. View Article : Google Scholar : PubMed/NCBI
15	Fernandez-Capetillo O, Chen HT, Celeste A, Ward I, Romanienko PJ, Morales JC, Naka K, Xia Z, Camerini-Otero RD, Motoyama N, et al: DNA damage-induced G2-M checkpoint activation by histone H2AX and 53BP1. Nat Cell Biol. 4:993–997. 2002. View Article : Google Scholar : PubMed/NCBI
16	Takasawa S, Nata K, Yonekura H and Okamoto H: Cyclic ADP-ribose in insulin secretion from pancreatic beta cells. Science. 259:370–373. 1993. View Article : Google Scholar : PubMed/NCBI
17	Guedes AG, Rude EP and Kannan MS: Potential role of the CD38/cADPR signaling pathway as an underlying mechanism of the effects of medetomidine on insulin and glucose homeostasis. Vet Anaesth Analg. 40:512–516. 2013. View Article : Google Scholar : PubMed/NCBI
18	Yamaguchi M and Kashiwakura I: Role of reactive oxygen species in the radiation response of human hematopoietic stem/progenitor cells. PLoS One. 8:e705032013. View Article : Google Scholar : PubMed/NCBI
19	Tessitore A, Cicciarelli G, Del Vecchio F, Gaggiano A, Verzella D, Fischietti M, Vecchiotti D, Capece D, Zazzeroni F and Alesse E: MicroRNAs in the DNA damage/repair network and cancer. Int J Genomics. 2014:8202482014. View Article : Google Scholar : PubMed/NCBI
20	Sun L, Iqbal J, Zaidi S, Zhu LL, Zhang X, Peng Y, Moonga BS and Zaidi M: Structure and functional regulation of the CD38 promoter. Biochem Biophys Res Commun. 341:804–809. 2006. View Article : Google Scholar : PubMed/NCBI
21	Yalçintepe L, Halis E and Ulku S: Effect of CD38 on the multidrug resistance of human chronic myelogenous leukemia K562 cells to doxorubicin. Oncol Lett. 11:2290–2296. 2016. View Article : Google Scholar : PubMed/NCBI